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The map label for this gene is aspA [H]

Identifier: 170682542

GI number: 170682542

Start: 1103143

End: 1104309

Strand: Reverse

Name: aspA [H]

Synonym: EcSMS35_1087

Alternate gene names: 170682542

Gene position: 1104309-1103143 (Counterclockwise)

Preceding gene: 170683393

Following gene: 170681818

Centisome position: 21.79

GC content: 47.81

Gene sequence:

>1167_bases
ATGTCTCGTATTGAAAGCGATTTCCTTGGTGAGCGTGAAATTCCGGATGATTGCTATTATGGTGTTCAGACACTTCGTGG
AAAAGATAATTTCCATATCACTGAAATGCCAATGAGTCAGGAGCCTTTCTTCATCATTGCCTTCGGTTATGTGAAAAAAG
CAGCTGCGATGGCTAATAAGGAGTTAGGTACAATTCCTGCTGATGTTGCGGATGCTTTAATCTGGGCTTGCGACCAATTA
ATTGATGGTAAGTACCGGGAACAGTTTGTGACCGACTGGCTTCAGGGGGGAGCCGGAACGTCAACCAATATGAACTGCAA
TGAAGTGATTTGTAATCTTGCTGCAGAAAAACTTGGTAGCGTTAAAGGGGATTACAAACGCGTTTCGCCAAACGACCATG
CTAATTTTGGTCAGTCCACCAATGATACATATCCGACAGCATTACATCTGGCATTGCTGCTGCGAAGCAATGTATTGCTT
GAAGCTGTTGAACACCTCGTAGGAGCATTCTACAAAAAGGCTGACGAGTTCAGCACAGTGCTCAAGATGGGACGTACTCA
CCTGCAGGATGCAGTGCCAATGACTCTCGGCCAGGAGTTCCATGGCTGGGGTTTTACAATTAATGATGAAATTCGGGTCA
TCCGTAATGCACAGGAACACCTGCGGGTTGTAAACCTTGGTGCTACTGCGATTGGTACCTGTGTTACCGCTCACCCGGAT
TATCCCGCTTTAGCCGTGAAATATCTGGCCCAGATCACAGGCATTAATTTCAGGAACAGTGAAGATCTCATCGCTGCCAC
AAGCGACTGTGGTGCATATGTTGCACTCAGTTCGGCCATGAAGAGCCTCTCTGTGAAGCTTACCAAGGTTTGTAATGACA
TTCGACTGCTTGCTTCAGGCCCCCGTTGCGGCCTGGCTGAAATTAACCTGCCTCAATTGCAACCGGGTTCTTCCATTATG
CCTGGTAAGGTTAACCCCGTTATCCCGGAAGTAACAAACCAGTCTTGCTTCCTGGTTCAGGGGCTGGACACCACAGTGAT
GCTGGCGGCATCTGCTGGTCAGCTTGAGCTTAACGTTATGTGGATTTGCCCCTATATTTCCAGACACCTGTTATCACTTA
ACCCATTACTGGCCTGCTGCCGCAGATATTCCCGTGGCGAGCGATAA

Upstream 100 bases:

>100_bases
TGTTATTTCAATGATGTAAGAATTCAAATTCCGAGAGCATAGCTCCGGTTAAACAATTTGTTATTGTCACATTGTTTTCA
AATAATGGAGTTAGAATATT

Downstream 100 bases:

>100_bases
CCCAGTGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGG
TTTAGGCATGATACTGATGT

Product: lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSRIESDFLGEREIPDDCYYGVQTLRGKDNFHITEMPMSQEPFFIIAFGYVKKAAAMANKELGTIPADVADALIWACDQL
IDGKYREQFVTDWLQGGAGTSTNMNCNEVICNLAAEKLGSVKGDYKRVSPNDHANFGQSTNDTYPTALHLALLLRSNVLL
EAVEHLVGAFYKKADEFSTVLKMGRTHLQDAVPMTLGQEFHGWGFTINDEIRVIRNAQEHLRVVNLGATAIGTCVTAHPD
YPALAVKYLAQITGINFRNSEDLIAATSDCGAYVALSSAMKSLSVKLTKVCNDIRLLASGPRCGLAEINLPQLQPGSSIM
PGKVNPVIPEVTNQSCFLVQGLDTTVMLAASAGQLELNVMWICPYISRHLLSLNPLLACCRRYSRGER

Sequences:

>Translated_388_residues
MSRIESDFLGEREIPDDCYYGVQTLRGKDNFHITEMPMSQEPFFIIAFGYVKKAAAMANKELGTIPADVADALIWACDQL
IDGKYREQFVTDWLQGGAGTSTNMNCNEVICNLAAEKLGSVKGDYKRVSPNDHANFGQSTNDTYPTALHLALLLRSNVLL
EAVEHLVGAFYKKADEFSTVLKMGRTHLQDAVPMTLGQEFHGWGFTINDEIRVIRNAQEHLRVVNLGATAIGTCVTAHPD
YPALAVKYLAQITGINFRNSEDLIAATSDCGAYVALSSAMKSLSVKLTKVCNDIRLLASGPRCGLAEINLPQLQPGSSIM
PGKVNPVIPEVTNQSCFLVQGLDTTVMLAASAGQLELNVMWICPYISRHLLSLNPLLACCRRYSRGER
>Mature_387_residues
SRIESDFLGEREIPDDCYYGVQTLRGKDNFHITEMPMSQEPFFIIAFGYVKKAAAMANKELGTIPADVADALIWACDQLI
DGKYREQFVTDWLQGGAGTSTNMNCNEVICNLAAEKLGSVKGDYKRVSPNDHANFGQSTNDTYPTALHLALLLRSNVLLE
AVEHLVGAFYKKADEFSTVLKMGRTHLQDAVPMTLGQEFHGWGFTINDEIRVIRNAQEHLRVVNLGATAIGTCVTAHPDY
PALAVKYLAQITGINFRNSEDLIAATSDCGAYVALSSAMKSLSVKLTKVCNDIRLLASGPRCGLAEINLPQLQPGSSIMP
GKVNPVIPEVTNQSCFLVQGLDTTVMLAASAGQLELNVMWICPYISRHLLSLNPLLACCRRYSRGER

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=358, Percent_Identity=41.8994413407821, Blast_Score=265, Evalue=7e-71,
Organism=Escherichia coli, GI87082375, Length=380, Percent_Identity=55.2631578947368, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787896, Length=379, Percent_Identity=39.5778364116095, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17553882, Length=378, Percent_Identity=42.5925925925926, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32565146, Length=242, Percent_Identity=39.6694214876033, Blast_Score=174, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6324993, Length=363, Percent_Identity=40.495867768595, Blast_Score=270, Evalue=3e-73,
Organism=Drosophila melanogaster, GI78710009, Length=383, Percent_Identity=41.77545691906, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24640179, Length=382, Percent_Identity=40.0523560209424, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24640177, Length=382, Percent_Identity=40.0523560209424, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24662684, Length=380, Percent_Identity=41.3157894736842, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24583245, Length=380, Percent_Identity=37.1052631578947, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 42539; Mature: 42408

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIESDFLGEREIPDDCYYGVQTLRGKDNFHITEMPMSQEPFFIIAFGYVKKAAAMANK
CCCCCCHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCC
ELGTIPADVADALIWACDQLIDGKYREQFVTDWLQGGAGTSTNMNCNEVICNLAAEKLGS
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
VKGDYKRVSPNDHANFGQSTNDTYPTALHLALLLRSNVLLEAVEHLVGAFYKKADEFSTV
CCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKMGRTHLQDAVPMTLGQEFHGWGFTINDEIRVIRNAQEHLRVVNLGATAIGTCVTAHPD
HHHHHHHHHHCCCHHHCCCCCCCCEEECCHHHHHHCHHHHHEEEEECCHHHHHEEECCCC
YPALAVKYLAQITGINFRNSEDLIAATSDCGAYVALSSAMKSLSVKLTKVCNDIRLLASG
CHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECC
PRCGLAEINLPQLQPGSSIMPGKVNPVIPEVTNQSCFLVQGLDTTVMLAASAGQLELNVM
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEE
WICPYISRHLLSLNPLLACCRRYSRGER
EECHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRIESDFLGEREIPDDCYYGVQTLRGKDNFHITEMPMSQEPFFIIAFGYVKKAAAMANK
CCCCCHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCC
ELGTIPADVADALIWACDQLIDGKYREQFVTDWLQGGAGTSTNMNCNEVICNLAAEKLGS
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
VKGDYKRVSPNDHANFGQSTNDTYPTALHLALLLRSNVLLEAVEHLVGAFYKKADEFSTV
CCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKMGRTHLQDAVPMTLGQEFHGWGFTINDEIRVIRNAQEHLRVVNLGATAIGTCVTAHPD
HHHHHHHHHHCCCHHHCCCCCCCCEEECCHHHHHHCHHHHHEEEEECCHHHHHEEECCCC
YPALAVKYLAQITGINFRNSEDLIAATSDCGAYVALSSAMKSLSVKLTKVCNDIRLLASG
CHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECC
PRCGLAEINLPQLQPGSSIMPGKVNPVIPEVTNQSCFLVQGLDTTVMLAASAGQLELNVM
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEE
WICPYISRHLLSLNPLLACCRRYSRGER
EECHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]