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The map label for this gene is rfaH [H]

Identifier: 170682425

GI number: 170682425

Start: 4319569

End: 4320057

Strand: Reverse

Name: rfaH [H]

Synonym: EcSMS35_4222

Alternate gene names: 170682425

Gene position: 4320057-4319569 (Counterclockwise)

Preceding gene: 170682404

Following gene: 170683184

Centisome position: 85.24

GC content: 50.92

Gene sequence:

>489_bases
ATGCAATCCTGGTATTTACTGTACTGCAAGCGCGGGCAACTTCAACGTGCCCAGGAACACCTCGAAAGACAGGCTGTGAA
TTGCCTGGCACCGATGATCTCCCTGGAAAAAATCGTGCGTGGCAAACGTACTGCAGTCAGTGAGCCATTGTTCCCCAACT
ACTTGTTTGTGGAGTTCGATCCGGAAGTGATTCATACCACGACTATCAACGCGACCCGCGGTGTCAGCCACTTCGTGCGC
TTTGGCGCGTCGCCAGCGATAGTCCCATCGGCGGTGATTCATCAGCTATCGGTATATAAACCGAAAGACATTGTCGATCC
GGCAACCCCTTATCCGGGTGATAAGGTGATTATTACCGAAGGCGCGTTCGAAGGTTTTCAGGCCATTTTCACCGAACCCG
ACGGCGAGGCTCGCTCCATGCTGTTACTTAATCTTATCAATAAAGAGATTAAGCACAGTGTGAAGAACACCGAGTTCCGC
AAACTCTAA

Upstream 100 bases:

>100_bases
AGCGTGAACTCTGACGGTATAACGCAAACCGGGGAATATAATTAACTTAGCGTAAAGCTTTTGCTATCCTTGCGCCCCGA
TTAAACGGATAAGAGTCATT

Downstream 100 bases:

>100_bases
AACGCAATCCCAAACAGTGTTTTAACATTGGCATCCGTGGTGGCAGCCAGCCATGCGGCATCTTCTCCACGCCAGTGCGC
AATACGTTGCAAAATATGGG

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMISLEKIVRGKRTAVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVR
FGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
KL

Sequences:

>Translated_162_residues
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMISLEKIVRGKRTAVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVR
FGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
KL
>Mature_162_residues
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMISLEKIVRGKRTAVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVR
FGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
KL

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=162, Percent_Identity=99.3827160493827, Blast_Score=337, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 18326; Mature: 18326

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMISLEKIVRGKRTAVSEPLFPNYLFVEFD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEC
PEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITE
CCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
GAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFRKL
CCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMISLEKIVRGKRTAVSEPLFPNYLFVEFD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEC
PEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITE
CCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
GAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFRKL
CCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]