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The map label for this gene is fadA [H]

Identifier: 170682404

GI number: 170682404

Start: 4322655

End: 4323818

Strand: Reverse

Name: fadA [H]

Synonym: EcSMS35_4226

Alternate gene names: 170682404

Gene position: 4323818-4322655 (Counterclockwise)

Preceding gene: 170684115

Following gene: 170682425

Centisome position: 85.31

GC content: 58.85

Gene sequence:

>1164_bases
ATGGAACAGGTTGTCATTGTCGATGCAATTCGCACCCCGATGGGCCGTTCGAAGGGCGGTGCTTTTCGTCACGTGCGTGC
GGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTT
ACTGGGGCTGTGTACAGCAGACGCTGGAGCAAGGTTTTAATATCGCCCGTAACGCGGCGCTGTTGGCAGAAGTACCACAC
TCTGTCCCGGCGGTGACCGTCAATCGCCTGTGTGGTTCATCCATGCAAGCATTACATGACGCAGCGCGAATGATCATGAC
CGGCGATGCGCAGGCATGTCTGGTTGGCGGCGTGGAGCATATGGGCCATGTGCCGATGAGTCACGGCGTCGATTTTCACC
CCGGCCTGAGCCGCAATGTCGCCAAAGCGGCGGGCATGATGGGCTTAACGGCAGAAATGCTGGCGCGTATGCACGGTATC
AGCCGTGAAATGCAGGATGCCTTTGCCGCGCGGTCACACGCCCGCGCCTGGGCCGCCACGCAGTCGGGTGCATTTAAAAA
TGAAATTATCCCGACCGGTGGTCACGATGCCGACGGCGTCCTGAAGCAGTTTAATTACGACGAAGTGATTCGCCCGGAAA
CCACCGTGGAAGCCCTCGCCACGCTGCGCCCGGCGTTTGATCCCGTCAGCGGTACGGTGACGGCAGGTACATCTTCTGCG
CTTTCCGATGGCGCAGCTGCCATGCTGGTGATGAGTGAAAGCCGCGCCCGAGAATTAGGTCTTAAGCCGCGCGCCCGCGT
GCGTTCGATGGCGGTCGTTGGTTGTGACCCATCGATTATGGGTTACGGCCCGGTTCCGGCCTCGAAGCTGGCGCTGAAAA
AAGCGGGGCTTTCTGCCAGCGATATCGGCGTATTCGAGATGAACGAAGCCTTTGCCGCGCAGATCCTGCCATGTATTAAA
GATCTCGGGCTAATGGAGCAGATTGACGAGAAGATCAACCTCAACGGTGGCGCGATCGCGCTGGGTCATCCGCTGGGTTG
TTCCGGTGCGCGTATCAGCACCACGCTGCTGAATCTGATGGAGCGCAAAGATGCCCAGTTTGGTCTGGCGACGATGTGTA
TCGGTCTGGGTCAGGGTATTGCGACGGTGTTTGAGCGGATTTAA

Upstream 100 bases:

>100_bases
GGAAGGTCTGCGTAATAAAGCGCGTCATAACGAACCGTACTATCCCCCGGTTGAGCCAGCCCGTCCGGTTGGCGACCTGA
AAACGGCTTAAGGAGTCACA

Downstream 100 bases:

>100_bases
CCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCTGCACCAATATAGGCCTGATAAGACGCGCCAGCG
TCGCATCAGGCATCGTGCAC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI

Sequences:

>Translated_387_residues
MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI
>Mature_387_residues
MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=43.1818181818182, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=38.7341772151899, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=39, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=34.032634032634, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=34.5771144278607, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=31.8066157760814, Blast_Score=149, Evalue=6e-36,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=97.9328165374677, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=45.1851851851852, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1788554, Length=405, Percent_Identity=43.4567901234568, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=41.645885286783, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1788683, Length=418, Percent_Identity=35.6459330143541, Blast_Score=194, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=41.3965087281795, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=33.953488372093, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.4987593052109, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=395, Percent_Identity=31.6455696202532, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=27.5773195876289, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=39.8976982097187, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=35.0490196078431, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=40.547263681592, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI17648125, Length=402, Percent_Identity=39.3034825870647, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=34.2657342657343, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=31.7733990147783, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40852; Mature: 40852

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEECHHHHHHCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDAQFGLATMCIGLGQGIATVFERI
HHHCCHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEECHHHHHHCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDAQFGLATMCIGLGQGIATVFERI
HHHCCHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA