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The map label for this gene is rcsF

Identifier: 170682244

GI number: 170682244

Start: 228727

End: 229080

Strand: Reverse

Name: rcsF

Synonym: EcSMS35_0208

Alternate gene names: 170682244

Gene position: 229080-228727 (Counterclockwise)

Preceding gene: 170680927

Following gene: 170683947

Centisome position: 4.52

GC content: 54.8

Gene sequence:

>354_bases
ATGTTAAGCAGATCCCCTGTCGAACCCGTTCAAAGCACTGCACCCCAGCCGAAAGCGGAGCCTGCAAAACCGAAAGCGCC
GCGCGCCACGCCGGTCCGAATTTATACCAATGCAGAAGAATTAGTCGGCAAACCGTTCCGCGATCTCGGTGAAGTCAGTG
GCGACTCTTGCCAGGCCTCTAATCAGGACTCTCCGCCGAGCATTCCAACCGCACGTAAGCGGATGCAAATCAACGCCTCG
AAAATGAAAGCCAATGCGGTATTACTGCATAGCTGCGAAGTCACCAGCGGTACGCCAGGCTGCTATCGTCAGGCTGTATG
TATCGGTTCTGCGCTTAACATCACGGCGAAATGA

Upstream 100 bases:

>100_bases
AAGCGCCTGATTCAATATTGACGTTTTGATCATACATTGAGGAAATACTATGCGTGCTTTACCGATCTGTTTAGTAGCAC
TCATGCTAAGCGGCTGTTCC

Downstream 100 bases:

>100_bases
GCAGTTTCCAGTTCGAGCAAATAGGCGTTATTCGCTCGCCCTATAAAGAAAAATTCGCCGTTCCGCGCCAGCCAGGTCTG
GTAAAAAGCGCCAACGGCGA

Product: outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MLSRSPVEPVQSTAPQPKAEPAKPKAPRATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINAS
KMKANAVLLHSCEVTSGTPGCYRQAVCIGSALNITAK

Sequences:

>Translated_117_residues
MLSRSPVEPVQSTAPQPKAEPAKPKAPRATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINAS
KMKANAVLLHSCEVTSGTPGCYRQAVCIGSALNITAK
>Mature_117_residues
MLSRSPVEPVQSTAPQPKAEPAKPKAPRATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINAS
KMKANAVLLHSCEVTSGTPGCYRQAVCIGSALNITAK

Specific function: Essential component of the Rcs signaling system to promote transcription of the genes for colanic acid capsule synthesis. Together with rcsC, they probably promote the phosphorylation of rcsB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786394, Length=117, Percent_Identity=100, Blast_Score=238, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RCSF_ECOL6 (P69412)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_752185.1
- ProteinModelPortal:   P69412
- EnsemblBacteria:   EBESCT00000044345
- GeneID:   1040431
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c0237
- GeneTree:   EBGT00050000010778
- HOGENOM:   HBG677406
- OMA:   ESCQASN
- ProtClustDB:   PRK10781

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 12403; Mature: 12403

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRSPVEPVQSTAPQPKAEPAKPKAPRATPVRIYTNAEELVGKPFRDLGEVSGDSCQAS
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECHHHHHCCCCHHHHHCCCCCCCCC
NQDSPPSIPTARKRMQINASKMKANAVLLHSCEVTSGTPGCYRQAVCIGSALNITAK
CCCCCCCCCCCHHHHCCCCCHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MLSRSPVEPVQSTAPQPKAEPAKPKAPRATPVRIYTNAEELVGKPFRDLGEVSGDSCQAS
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECHHHHHCCCCHHHHHCCCCCCCCC
NQDSPPSIPTARKRMQINASKMKANAVLLHSCEVTSGTPGCYRQAVCIGSALNITAK
CCCCCCCCCCCHHHHCCCCCHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Stimulates colanic acid capsule synthesis [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157