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The map label for this gene is gcp [H]

Identifier: 170682206

GI number: 170682206

Start: 3435048

End: 3436061

Strand: Reverse

Name: gcp [H]

Synonym: EcSMS35_3357

Alternate gene names: 170682206

Gene position: 3436061-3435048 (Counterclockwise)

Preceding gene: 170682273

Following gene: 170682678

Centisome position: 67.79

GC content: 56.8

Gene sequence:

>1014_bases
ATGCGTGTACTGGGTATTGAAACTTCCTGCGATGAAACCGGCATCGCCATTTACGACGATGAAAAAGGTTTGTTAGCCAA
CCAATTGTATAGTCAGGTGAAATTGCACGCTGACTACGGCGGCGTCGTGCCTGAACTGGCCTCCCGCGATCACGTGCGTA
AAACCGTACCGTTGATCCAGGAGGCGCTGAAAGAGTCTGGTTTAACGGCAAAAGACATTGATGCTGTGGCCTATACCGCA
GGCCCAGGATTAGTCGGCGCACTGCTGGTTGGCGCGACCGTGGGGCGTTCTCTGGCGTTTGCCTGGGACGTTCCGGCAAT
CCCGGTACACCATATGGAAGGGCATCTGTTAGCGCCGATGCTGGAAGATAACCCACCGGCATTTCCGTTTGTGGCGCTGC
TGGTTTCCGGCGGTCATACGCAGTTAATCAGCGTGACTGGCATTGGTCAGTACGAGCTGCTTGGCGAGTCTATCGATGAT
GCTGCTGGTGAAGCGTTTGATAAAACCGCGAAGCTGCTGGGGCTGGATTATCCTGGCGGGCCATTACTGTCGAAAATGGC
GGCTCAGGGTACTGCCGGGCGCTTTGTCTTCCCGCGCCCGATGACCGACCGTCCGGGGCTGGATTTCAGCTTTTCTGGTC
TGAAAACCTTCGCGGCGAACACGATTCGTGACAACGGCACCGACGACCAGACGCGTGCTGATATCGCCCGCGCCTTTGAA
GATGCGGTGGTCGATACGTTGATGATTAAGTGTAAGCGAGCACTGGATCAGACGGGCTTTAAGCGACTGGTAATGGCAGG
CGGCGTGAGTGCTAACCGCACGTTACGGGCGAAGCTGGCTGAAATGATGAAAAAACGCCGTGGCGAAGTGTTCTACGCGC
GTCCGGAATTTTGTACTGATAACGGCGCGATGATCGCCTATGCCGGAATGGTGCGGTTTAAAGCAGGCGCGACGGCGGAT
CTCGGCGTTAGCGTGCGTCCGCGCTGGCCGCTGGCAGAGTTACCGGCCGCGTAA

Upstream 100 bases:

>100_bases
TGAGGAGTATACACGAGGCTTTCTCCAGGGGCGAACAAAGTTTTACATCAACCCGCATTGGTCCTACACTGCGCGGTAAT
AAAGCGAGGTAAAACAAGTC

Downstream 100 bases:

>100_bases
AACTATTAGGTGCCGGAGAGTCACTTCCGGCACCGTCCTCACTTAAAGCAACACCACGGGCATCCACAGTAAGCCGGTAA
TCACCAGCAATACGAGGAAT

Product: putative DNA-binding/iron metalloprotein/AP endonuclease

Products: NA

Alternate protein names: Glycoprotease [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVPELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTA
GPGLVGALLVGATVGRSLAFAWDVPAIPVHHMEGHLLAPMLEDNPPAFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFAANTIRDNGTDDQTRADIARAFE
DAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLRAKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATAD
LGVSVRPRWPLAELPAA

Sequences:

>Translated_337_residues
MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVPELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTA
GPGLVGALLVGATVGRSLAFAWDVPAIPVHHMEGHLLAPMLEDNPPAFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFAANTIRDNGTDDQTRADIARAFE
DAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLRAKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATAD
LGVSVRPRWPLAELPAA
>Mature_337_residues
MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVPELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTA
GPGLVGALLVGATVGRSLAFAWDVPAIPVHHMEGHLLAPMLEDNPPAFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFAANTIRDNGTDDQTRADIARAFE
DAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLRAKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATAD
LGVSVRPRWPLAELPAA

Specific function: Could Be A Metalloprotease. [C]

COG id: COG0533

COG function: function code O; Metal-dependent proteases with possible chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M22 family [H]

Homologues:

Organism=Homo sapiens, GI116812636, Length=356, Percent_Identity=34.8314606741573, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI8923380, Length=332, Percent_Identity=35.5421686746988, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1789445, Length=337, Percent_Identity=99.406528189911, Blast_Score=680, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17557464, Length=327, Percent_Identity=31.4984709480122, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71995670, Length=329, Percent_Identity=34.6504559270517, Blast_Score=150, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6320099, Length=366, Percent_Identity=33.0601092896175, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322891, Length=348, Percent_Identity=28.1609195402299, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI20129063, Length=337, Percent_Identity=34.4213649851632, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI21357207, Length=329, Percent_Identity=29.7872340425532, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022450
- InterPro:   IPR000905
- InterPro:   IPR017860
- InterPro:   IPR017861 [H]

Pfam domain/function: PF00814 Peptidase_M22 [H]

EC number: =3.4.24.57 [H]

Molecular weight: Translated: 36009; Mature: 36009

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS01016 GLYCOPROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVPELASRDHVRKTVPLIQ
CEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHEEECCCCCCCHHHHCHHHHHHHHHHHH
EALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAWDVPAIPVHHMEGHLLAPM
HHHHHCCCCHHHCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEECCH
LEDNPPAFPFVALLVSGGHTQLISVTGIGQYELLGESIDDAAGEAFDKTAKLLGLDYPGG
HCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
PLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFAANTIRDNGTDDQTRADIARAFE
HHHHHHHHCCCCCCEECCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLRAKLAEMMKKRRGEVFYARPEFCTD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEECC
NGAMIAYAGMVRFKAGATADLGVSVRPRWPLAELPAA
CCCEEEEEEEEEECCCCCCCCCEEECCCCCHHCCCCC
>Mature Secondary Structure
MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVPELASRDHVRKTVPLIQ
CEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHEEECCCCCCCHHHHCHHHHHHHHHHHH
EALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAWDVPAIPVHHMEGHLLAPM
HHHHHCCCCHHHCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEECCH
LEDNPPAFPFVALLVSGGHTQLISVTGIGQYELLGESIDDAAGEAFDKTAKLLGLDYPGG
HCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
PLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFAANTIRDNGTDDQTRADIARAFE
HHHHHHHHCCCCCCEECCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLRAKLAEMMKKRRGEVFYARPEFCTD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEECC
NGAMIAYAGMVRFKAGATADLGVSVRPRWPLAELPAA
CCCEEEEEEEEEECCCCCCCCCEEECCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA