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The map label for this gene is yijO

Identifier: 170682151

GI number: 170682151

Start: 4493650

End: 4494501

Strand: Reverse

Name: yijO

Synonym: EcSMS35_4400

Alternate gene names: 170682151

Gene position: 4494501-4493650 (Counterclockwise)

Preceding gene: 170680859

Following gene: 170679892

Centisome position: 88.68

GC content: 53.17

Gene sequence:

>852_bases
ATGTATCACGACGTCAGCTATCTGCTCTCCCGCCTGATCAACGGCCCGCTGTCCCTGCGCCAGATTTACTTTGCCAGCAG
TAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGACTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATA
CCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACGTTCCCGCTGGTGGCTGGAATTTTCCACAATGGCAAGCC
CCCGCCACTACCTTTAGCGTGCTGTTTGGCAAACAGCAACTCGGCTTCAGCGTCGTGCAATGGGATGGCAAACAATATCA
AAATCTGGCGAAGCAACACGTCGCCCGACGCGGCCCGCGCATTGGTTCTTTTCTGCTACAAACGCTCAATGAAATGCAAA
TGCAGCCGCAGGAGCAGCAAACGGCAAGGCTTATCGTCGCCAGCCTGCTTAGCCACTGCCGCGATTTGCTCGGCAGCCAA
ATCCAGACCGCCTCACGTAGCCAGGCTCTATTCGAAGCTATTCGCGATTATATCGACGAACGCTATGCCTCCGCGCTTAC
CCGCGAATCTGTCGCACAGGCATTCTATATTTCGCCAAATTACCTCTCGCACCTGTTTCAAAAAACGGGGGCCATTGGTT
TTAACGAATACCTGAATCACACGCGACTGGAGCACGCTAAGACGTTATTGAAAGGCTATGACCTGAAGGTAAAAGAAGTG
GCGCACGCCTGCGGCTTTGTCGACAGCAACTACTTCTGCCGTCTGTTTCGTAAAAATACCGAACGCTCACCGTCGGAATA
TCGTCGGCAGTACCACAGCCAACTGACAGAAAAACCGACTACTCCAGAATAA

Upstream 100 bases:

>100_bases
GCAATGTTTACATCGAACTCCCCTCAGGCCACACTTTTCAGGTGGCCTTTTTTCATAATGCCAGTTACTGTTTTCCCGTC
TACATACCTAAGGGAATAAC

Downstream 100 bases:

>100_bases
GATGGGTTTGCTGCGGCGCAGAAAGCACTTTGCGTACTGCGCTCATCACCCGCTGCGGCTCACGCAGGAAAGCGTAGATG
CTGCATTGCACGTAGCGGCA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLEIVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQA
PATTFSVLFGKQQLGFSVVQWDGKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYLNHTRLEHAKTLLKGYDLKVKEV
AHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLTEKPTTPE

Sequences:

>Translated_283_residues
MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLEIVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQA
PATTFSVLFGKQQLGFSVVQWDGKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYLNHTRLEHAKTLLKGYDLKVKEV
AHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLTEKPTTPE
>Mature_283_residues
MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLEIVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQA
PATTFSVLFGKQQLGFSVVQWDGKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYLNHTRLEHAKTLLKGYDLKVKEV
AHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLTEKPTTPE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790391, Length=283, Percent_Identity=100, Blast_Score=589, Evalue=1e-170,
Organism=Escherichia coli, GI1788725, Length=270, Percent_Identity=35.5555555555556, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1790339, Length=162, Percent_Identity=27.1604938271605, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YIJO_ECOLI (P32677)

Other databases:

- EMBL:   U00006
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E65202
- RefSeq:   AP_003856.1
- RefSeq:   NP_418389.1
- ProteinModelPortal:   P32677
- STRING:   P32677
- EnsemblBacteria:   EBESCT00000000338
- EnsemblBacteria:   EBESCT00000016170
- GeneID:   948451
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3926
- KEGG:   eco:b3954
- EchoBASE:   EB1857
- EcoGene:   EG11913
- eggNOG:   COG2207
- GeneTree:   EBGT00050000008635
- HOGENOM:   HBG416924
- OMA:   IPPNCWN
- ProtClustDB:   CLSK880821
- BioCyc:   EcoCyc:EG11913-MONOMER
- Genevestigator:   P32677
- GO:   GO:0005622
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710
- Gene3D:   G3DSA:1.10.10.60
- Gene3D:   G3DSA:2.60.120.10
- PRINTS:   PR00032
- SMART:   SM00342

Pfam domain/function: PF00165 HTH_AraC; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 32150; Mature: 32150

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLEIVLEGEFVDTGAGATL
CCCCHHHHHHHHHCCCHHHHHEEEECCCCCCCCCEEEECCCEEEEEEECCEEECCCCCEE
VPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWDGKQYQNLAKQHVARRGPR
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCH
IGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQIQTASRSQALFEAIRDYIDE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYLNHTRLEHAKTLLKGYDLKVKEV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
AHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLTEKPTTPE
HHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLEIVLEGEFVDTGAGATL
CCCCHHHHHHHHHCCCHHHHHEEEECCCCCCCCCEEEECCCEEEEEEECCEEECCCCCEE
VPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWDGKQYQNLAKQHVARRGPR
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCH
IGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQIQTASRSQALFEAIRDYIDE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYLNHTRLEHAKTLLKGYDLKVKEV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
AHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLTEKPTTPE
HHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503