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The map label for this gene is gldA [H]

Identifier: 170682139

GI number: 170682139

Start: 4484116

End: 4485219

Strand: Reverse

Name: gldA [H]

Synonym: EcSMS35_4393

Alternate gene names: 170682139

Gene position: 4485219-4484116 (Counterclockwise)

Preceding gene: 170680048

Following gene: 170683775

Centisome position: 88.49

GC content: 54.44

Gene sequence:

>1104_bases
ATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCC
ACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGAAAAGCTTTAAAG
ATGCTGGACTGGTAGTAGAAATTGCGCCGTTTGGCGGTGAATGTTCGCAAAATGAGATCGACCGTCTGCGTGGCATCGCG
GAGACTGCGCAGTGTGGCGCAATTCTCGGTATCGGTGGCGGAAAAACCCTCGATACTGCCAAAGCACTGGCACATTTCAT
GGGTGTTCCGGTAGCGATCGCACCGACTATCGCCTCTACCGACGCACCGTGCAGTGCGTTGTCTGTTATCTACACCGATG
AGGGTGAGTTTGACCGCTATCTGATGTTGCCAAATAACCCGAATATGGTCATTGTCGACACCAAAATCGTCGCTGGCGCA
CCTGCACGTCTGTTAGCGGCGGGTATCGGCGATGCGCTGGCAACCTGGTTTGAAGCACGCGCCTGTTCCCGTAGCGGCGC
TACCACCATGGCGGGCGGCAAGTGCACTCAGGCGGCGCTGGCACTGGCAGAACTGTGCTACAACACTCTGCTGGAAGAAG
GCGAAAAAGCGATGCTTGCCGCCGAACAGCATGTAGTGACTCCGGCGCTGGAACGCGTGATTGAAGCGAACACCTATTTG
AGCGGTGTTGGTTTTGAAAGCGGCGGGCTGGCGGCGGCACACGCGGTACATAACGGCCTGACCGCTATCCCGGACGCGCA
TCACTATTATCATGGTGAAAAAGTGGCATTTGGTACGCTGACGCAGCTGGTACTGGAAAATGCGCCGGTGGAGGAAATCG
AAACCGTAGCTGCCCTTAGCCATGCGGTAGGTTTGCCAATAACGCTCGCTCAACTGGATATTAAAGAAGATGTCCCGGCG
AAAATGCGAATTGTGGCAGAAGCGGCATGTGCAGAAGGTGAAACCATCCACAACATGCCTGGCGGCGCAACGCCAGATCA
GGTTTACGCCGCACTGCTGGTAGCTGACCAGTATGGACAACGTTTCCTGCAAGAGTGGGAATAA

Upstream 100 bases:

>100_bases
CAACAAATGCTCAACACCCCTGCGGTAGAGTCAGCTATAGAGAAGTTCGAACACGACTGGAATGCCGCATTTGGCACTAC
TCATCTCTAAAGGAGCAATT

Downstream 100 bases:

>100_bases
CCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATC
TCTGCAATATATTGAATTTG

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLMLPNNPNMVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPA
KMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQRFLQEWE

Sequences:

>Translated_367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLMLPNNPNMVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPA
KMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQRFLQEWE
>Mature_367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLMLPNNPNMVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPA
KMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQRFLQEWE

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=367, Percent_Identity=99.7275204359673, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1786815, Length=359, Percent_Identity=31.7548746518106, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 38731; Mature: 38731

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIAST
EECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCC
DAPCSALSVIYTDEGEFDRYLMLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEAR
CCCCCEEEEEEECCCCCCCEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCE
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALS
ECCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHH
HAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQ
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
RFLQEWE
HHHHHCC
>Mature Secondary Structure
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIAST
EECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCC
DAPCSALSVIYTDEGEFDRYLMLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEAR
CCCCCEEEEEEECCCCCCCEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCE
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALS
ECCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHH
HAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQ
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
RFLQEWE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]