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The map label for this gene is glcA

Identifier: 170682106

GI number: 170682106

Start: 3327708

End: 3329390

Strand: Reverse

Name: glcA

Synonym: EcSMS35_3252

Alternate gene names: 170682106

Gene position: 3329390-3327708 (Counterclockwise)

Preceding gene: 170681765

Following gene: 170683116

Centisome position: 65.69

GC content: 53.48

Gene sequence:

>1683_bases
ATGGTTACCTGGACCCAAATGTATATGCCGATGGGAGGACTGGGGCTATCCGCTCTGGTCGCCCTGATCCCGATAATATT
CTTCTTCGTTGCACTCGCGGTATTACGTCTGAAAGGACATGTCGCTGGAGCAATAACCCTTATATTATCTATCCTGATTG
CAATATTCGCCTTTAAAATGCCGATTGATATGGCATTTGCTGCTGCGGGCTATGGCTTTATTTATGGATTATGGCCAATA
GCGTGGATTATTGTCGCGGCGGTGTTCCTGTATAAATTAACCGTTGCCAGCGGGCAGTTCGATATTATCCGCAGCTCGGT
TATCTCCATCACCGACGATCAGCGTTTGCAGGTGTTACTGATTGGTTTCTCCTTTGGTGCGTTGCTGGAAGGAGCGGCTG
GCTTTGGTGCGCCGGTGGCGATTACCGGTGCGCTGCTGGTGGGCCTGGGCTTCAAACCGTTATACGCGGCGGGGCTGTGT
CTGATTGCCAATACTGCGCCGGTGGCGTTTGGTGCGTTGGGCGTGCCGATTCTGGTCGCCGGTCAGGTAACGGGAATCGA
TCCGTTCCACATTGGCGCAATGGCGGGACGTCAGTTACCGTTCCTGTCGGTTCTTGTGCCGTTCTGGCTGGTAGCAATGA
TGGACGGCTGGAAAGGGGTGAAAGAGACGTGGCCAGCGGCGCTGGTTGCTGGGGGAAGCTTCGCTGTCACTCAGTTCTTT
ACCTCTAACTATATTGGTCCGGAACTGCCGGATATTACTTCGGCGCTGGTGAGTATCGTCTCACTCGCTTTATTCCTTAA
AGTCTGGCGGCCGAAAAATACCGAAACGGCAATCAGCATGGGACAATCCGCAGGTGCGATGGTGGTAAATAAGCCATCTT
CTGGCGGTCCCGTGCCTTCAGAATATAGTCTGGGGCAAATCATTCGAGCGTGGTCACCGTTTTTAATCTTAACGGTGCTG
GTCACCATCTGGACCATGAAGCCGTTTAAAGCGTTATTTGCTCCGGGCGGCGCGTTTTATTCACTGGTGATTAATTTCCA
GATCCCTCATTTGCATCAACAAGTGTTGAAAGCGGCACCCATTGTCGCCCAACCAACGCCAATGGATGCGGTGTTTAAAT
TCGACCCCCTCTCGGCTGGCGGCACCGCTATTTTTATTGCGGCGATTATCTCTATCTTCATCCTCGGTGTGGGGATCAAG
AAAGGTATTGGCGTCTTTGCCGAAACGCTAATTAGCTTGAAGTGGCCGATACTGTCGATTGGCATGGTGCTGGCGTTCGC
CTTCGTCACCAACTATTCTGGCATGTCCACCACGCTGGCGCTGGTACTGGCAGGTACAGGCGTGATGTTCCCGTTCTTCT
CACCGTTTCTCGGCTGGCTGGGCGTATTCCTTACCGGCTCGGACACCTCCTCTAACGCCCTGTTTGGTTCACTGCAATCG
ACCACGGCGCAGCAAATCAACGTCTCTGACACCCTGCTGGTGGCAGCAAACACCAGCGGCGGCGTAACTGGCAAGATGAT
CTCCCCGCAATCTATCGCCGTGGCCTGCGCCGCGACGGGCATGGTGGGCCGAGAATCTGAACTGTTCCGCTACACCGTGA
AGCACAGTCTGATTTTTGCCAGCGTTATCGGCATTATCACCCTGCTGCAGGCGTATGTGTTTACCGGGATGTTAGTCTCG
TAA

Upstream 100 bases:

>100_bases
GCTGTAAAGTATAACGCCTATATTTTACAGTTCAGTCCTCTTTTGCTTATTACAACCTTAAGTATATCAAGCATATAAAG
ATAATAAGAGACTGAACAAT

Downstream 100 bases:

>100_bases
ATCAATAAAGCCGGATGATCGTTAATATCATCCGGCTTCATTTACATTATTCTGTCTTATTAATTGAAAATCATTTCTCG
CTTTATTATCCATGCAGCAA

Product: glycolate transporter

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPI
AWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFF
TSNYIGPELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVL
VTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIK
KGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQS
TTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS

Sequences:

>Translated_560_residues
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPI
AWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFF
TSNYIGPELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVL
VTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIK
KGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQS
TTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS
>Mature_560_residues
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPI
AWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFF
TSNYIGPELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVL
VTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIK
KGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQS
TTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family

Homologues:

Organism=Escherichia coli, GI1789347, Length=560, Percent_Identity=100, Blast_Score=1092, Evalue=0.0,
Organism=Escherichia coli, GI1790031, Length=558, Percent_Identity=63.0824372759857, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLCA_ECOLI (Q46839)

Other databases:

- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E65083
- RefSeq:   AP_003529.1
- RefSeq:   NP_417449.1
- ProteinModelPortal:   Q46839
- DIP:   DIP-12206N
- STRING:   Q46839
- PRIDE:   Q46839
- EnsemblBacteria:   EBESCT00000002237
- EnsemblBacteria:   EBESCT00000015674
- GeneID:   947259
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2942
- KEGG:   eco:b2975
- EchoBASE:   EB2818
- EcoGene:   EG12995
- eggNOG:   COG1620
- GeneTree:   EBGT00050000008813
- HOGENOM:   HBG289840
- OMA:   PIAWIVI
- ProtClustDB:   PRK09695
- BioCyc:   EcoCyc:B2975-MONOMER
- Genevestigator:   Q46839
- InterPro:   IPR003804
- TIGRFAMs:   TIGR00795

Pfam domain/function: PF02652 Lactate_perm

EC number: NA

Molecular weight: Translated: 58921; Mature: 58921

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b8a1c34)-; HASH(0x1c19b560)-; HASH(0x1be82ca8)-; HASH(0x1bd6a8a8)-; HASH(0x1c00b360)-; HASH(0x1c276d80)-; HASH(0x1c00b3e4)-; HASH(0x1beed480)-; HASH(0x1c261834)-; HASH(0x1c26a7f4)-; HASH(0x1c27f74c)-; HASH(0x1b8aaba8)-; HASH(0x1909da30)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKM
CCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEE
IGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLCLIANTAPVAFGALGVPILVA
EEECHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEECCCCHHHHHCCCCEEEE
GQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFF
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCHHEEECCCHHHHHHH
TSNYIGPELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEECCCCCCCCCC
EYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAP
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHCC
IVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAETLISLKWPILSI
CCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHH
GMVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQS
HHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCCHHEECHHH
TTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSLIFA
CHHHEECCCCEEEEEECCCCCCEECEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SVIGIITLLQAYVFTGMLVS
HHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKM
CCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEE
IGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLCLIANTAPVAFGALGVPILVA
EEECHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEECCCCHHHHHCCCCEEEE
GQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFF
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCHHEEECCCHHHHHHH
TSNYIGPELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEECCCCCCCCCC
EYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAP
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHCC
IVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAETLISLKWPILSI
CCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHH
GMVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQS
HHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCCHHEECHHH
TTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSLIFA
CHHHEECCCCEEEEEECCCCCCEECEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SVIGIITLLQAYVFTGMLVS
HHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976