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The map label for this gene is fitE [H]

Identifier: 170681989

GI number: 170681989

Start: 3395654

End: 3396601

Strand: Reverse

Name: fitE [H]

Synonym: EcSMS35_3319

Alternate gene names: 170681989

Gene position: 3396601-3395654 (Counterclockwise)

Preceding gene: 170683833

Following gene: 170681276

Centisome position: 67.02

GC content: 49.26

Gene sequence:

>948_bases
ATGCGTTTATTTTTTTCTCTGCTGATACTGTTGTCATTTTTTTCCCGTGCAACAGAACCTGTTCAGGTATTTACTGATGA
TTTAGGGCGCAAAGTCACTGTTCCTGCTCATCCAAAGAGAATTGTCTCTTTACACGATCTTGATATCACTATTCCTCTGA
TTGAGCTCGGTGTACCTCCTGTCGCCAGCCACGGGCGAACTCGGCCTGACGGTAGCCATTTCATTCGATCTGGCGCGTTA
TTGACAGGTGTCGACTTTGATAATTCATCGATCGCCTTTATTGGTACCGCTGATATTGATATTGAAGCGATTGTGGCGGC
AAAGCCTGACCTGATCATCACAGAACCGACCCGTAATACGCCCATTGAACAACTTGAAAAAATTGCGCCCACGGTGAGCA
TTGATCATCTCAAAGGGGGCGCGCCAGAAATCTATCGCAAGCTGGCAGAACTTACAGGAACGCAGTCGCAGCTGGCGATA
CTTGAACGCCGCTATCAGGCACAAATCAATGCGCTGAAGGCGACGCTGGACAGCCAAAAAATAACGGTATCGGTGATTCA
GGCAAACCAGGGAAAAATTAATGTGATGCATAGCTATCATTCGCTGGGGCGGGTATTACGCGATGCTGGTTTTCGTTTTC
CGCCGCTGATTGAGAGCATTCCTGAAGGTGGGCGAATGGATGTGAGCGCGGAGCGTTTACCTGAACTGGATGCCGATTTT
GTTTTTGCAACCTGGCGCGGTGACACCGGTGGTAAACCTCAGGATGAGCTGGCAGCAATGGAAAAAGTCATGCCGGGATG
GTGTCAGTTTCTGACTGCCTGTCGTTCCGGGCGTTATGTGCTGATTTCTCGTGAAGAGGCGATTTCTAACTCCTTTGCAT
CTCTGGGGCTGATGGCTGCGCAGATACAATCACAAATTGCAGGGCGACCTCTACCGGAGGATAAATGA

Upstream 100 bases:

>100_bases
CCTTTGCAACTGCCTGCCGGAATCATTATCTCACTGATTGGTGGACCATTTTTTTTGCTACTGCTCTGGCAGCGCAGGAA
CAGTTTTTAACGGGAGTTAT

Downstream 100 bases:

>100_bases
TCCAGAAAGATAAAGCGCGGGTTGATATTTTTGGTGAGCGTTTTCGCACTCGCGCGAGTCAGTTAACGCCTGGGTTAAGA
GTTGTCGCCAGCTACATTAA

Product: iron ABC transporter periplasmic iron-binding protein FitE

Products: ADP; phosphate; ferric enterobactin [Cytoplasm] [C]

Alternate protein names: Ferric hydroxamate uptake protein D; Ferrichrome-binding protein; Iron(III)-hydroxamate-binding protein fhuD [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSLHDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGAL
LTGVDFDNSSIAFIGTADIDIEAIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIESIPEGGRMDVSAERLPELDADF
VFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRSGRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEDK

Sequences:

>Translated_315_residues
MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSLHDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGAL
LTGVDFDNSSIAFIGTADIDIEAIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIESIPEGGRMDVSAERLPELDADF
VFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRSGRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEDK
>Mature_315_residues
MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSLHDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGAL
LTGVDFDNSSIAFIGTADIDIEAIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIESIPEGGRMDVSAERLPELDADF
VFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRSGRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEDK

Specific function: Part of the ABC transporter complex fhuCBGD involved in iron(3+)-hydroxamate import. Binds the iron(3+)-hydroxamate complex and transfers it to the membrane-bound permease. Required for the transport of ferrichrome and coprogen [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 34520; Mature: 34520

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSLHDLDITIPLIELGVPP
CHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCCCCEEEEECCEEEEEHHHCCCCC
VASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIEAIVAAKPDLIITEPTRNT
CCCCCCCCCCHHHHHHCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCEEEECCCCCC
PIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAILERRYQAQINALKATLDSQK
CHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
ITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIESIPEGGRMDVSAERLPELDADF
EEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCCE
VFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRSGRYVLISREEAISNSFASLGLMAA
EEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHH
QIQSQIAGRPLPEDK
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSLHDLDITIPLIELGVPP
CHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCCCCEEEEECCEEEEEHHHCCCCC
VASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIEAIVAAKPDLIITEPTRNT
CCCCCCCCCCHHHHHHCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCEEEECCCCCC
PIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAILERRYQAQINALKATLDSQK
CHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
ITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIESIPEGGRMDVSAERLPELDADF
EEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCCE
VFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRSGRYVLISREEAISNSFASLGLMAA
EEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHH
QIQSQIAGRPLPEDK
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ferric enterobactin [Periplasm]; H2O [C]

Specific reaction: ATP + ferric enterobactin [Periplasm] + H2O = ADP + phosphate + ferric enterobactin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8388528; 9639930; 9384377 [H]