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The map label for this gene is trmA [H]

Identifier: 170681986

GI number: 170681986

Start: 4507994

End: 4509094

Strand: Reverse

Name: trmA [H]

Synonym: EcSMS35_4412

Alternate gene names: 170681986

Gene position: 4509094-4507994 (Counterclockwise)

Preceding gene: 170680730

Following gene: 170681015

Centisome position: 88.97

GC content: 50.77

Gene sequence:

>1101_bases
ATGACCCCCGAACACCTTCCAACAGAACAGTATGAAGCGCAGTTAGCCGAAAAAGTGGTACGTTTGCAAAGTATGATGGC
ACCGTTTTCTGACCTGGTTCCGGAAGTGTTTCGCTCGCCGGTCAGTCATTACCGGATGCGTGCGGAGTTCCGCATCTGGC
ACGATGGCGATGACCTGTATCACATCATTTTCGATCAACAAACCAAAAGCCGCATCCGCGTGGATAGCTTCCCCGCCGCC
AGTGAACTTATCAACCAGTTGATGACGGCGATGATTGCGGGTGTGCGTAATAATCCCGTTCTGCGCCACAAGTTGTTCCA
GATTGATTACCTCACCACGCTGAGTAATCAGGCGGTGGTTTCCCTGCTGTACCATAAGAAGCTGGATGATGAGTGGCGTC
AGGAAGCGGAAGCCCTGCGCGACGCACTGCGAGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAA
ATCGAGCTGGATCAAGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAG
CTTTACCCAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTCAAAAGGCGATT
TACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCACTGGCGCGCAATTTTGATCGGGTATTAGCCACCGAAATC
GCTAAGCCGTCGGTCGCTGCCGCGCAATACAACATTGCCGCTAACCATATTGATAACGTACAAATTATTCGTATGGCGGC
AGAAGAATTTACTCAGGCGATGAATGGCGTACGCGAGTTTAACCGCCTGCAAGGGATCGATTTAAAGAGTTATCAGTGCG
AAACCATTTTTGTCGACCCACCACGCAGCGGTCTGGACGGTGAAACCGAGAAAATGGTGCAGGCGTATCCACGTATTCTG
TATATCTCCTGCAATCCGGAAACGTTATGCAAGAATCTGGAAACATTAAGTCAGACGCACAAGGTCGAACGTCTGGCTCT
GTTTGATCAGTTCCCCTACACGCACCATATGGAGTGCGGCGTATTACTGACCGCGAAGTAA

Upstream 100 bases:

>100_bases
ACTGTCCAGACGTAGCTCACAAATAGGAATTCATCAAGATCTGGACATCTGATGAGCAATCCCTACAATCGCCGCGTACT
TTAATTTTTCAGGATACATC

Downstream 100 bases:

>100_bases
AACCCATGCCGGATGCATCAGCATCCGGCATGATACCGATTACTCAGCAACTTCCTGCTTACGGTTACGCATCTTCGCGC
CAATCCAGAACACCATAATG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAA
SELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRIL
YISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECGVLLTAK

Sequences:

>Translated_366_residues
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAA
SELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRIL
YISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECGVLLTAK
>Mature_365_residues
TPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAAS
ELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKI
ELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEIA
KPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRILY
ISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECGVLLTAK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790403, Length=366, Percent_Identity=99.7267759562842, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=183, Percent_Identity=33.879781420765, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787083, Length=182, Percent_Identity=29.6703296703297, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24666579, Length=194, Percent_Identity=29.3814432989691, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 41937; Mature: 41806

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLY
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHEEEEEEEEEECCCCEE
HIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVV
EEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHH
SLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKIELDQDYIDERLPVAGKEMI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCHHHHHCCCCCCHHHHH
YRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
HHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDP
CCCCHHHHEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
PRSGLDGETEKMVQAYPRILYISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECG
CCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VLLTAK
EEEEEC
>Mature Secondary Structure 
TPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLY
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHEEEEEEEEEECCCCEE
HIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVV
EEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHH
SLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKIELDQDYIDERLPVAGKEMI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCHHHHHCCCCCCHHHHH
YRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
HHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDP
CCCCHHHHEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
PRSGLDGETEKMVQAYPRILYISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECG
CCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VLLTAK
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA