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The map label for this gene is hycE [H]

Identifier: 170681957

GI number: 170681957

Start: 2920654

End: 2922384

Strand: Reverse

Name: hycE [H]

Synonym: EcSMS35_2846

Alternate gene names: 170681957

Gene position: 2922384-2920654 (Counterclockwise)

Preceding gene: 170681545

Following gene: 170682605

Centisome position: 57.66

GC content: 56.67

Gene sequence:

>1731_bases
GTGATTAAAGAGAGTTTGAGCATGTCTGAAGAAAAATTAGGTCAACATTATCTCGCCGCGCTGAATGAGGCATTTCCGGG
CGTCGTGCTGGACCACGCCTGGCAGACCAAAGATCAGCTAACCGTCACCGTGAAGGTGAACTACCTGCCGGAAGTGGTGG
AGTTTCTCTACTACAAACAAGGGGGCTGGCTGTCGGTGCTGTTTGGTAACGACGAACGCAAACTGAATGGTCATTACGCC
GTTTACTACGTGCTGTCGATGGAGAAGGGCACCAAATGCTGGATAACCGTGCGCGTCGAAGTTGACGCCAACAAACCGGA
ATATCCGTCCGTGACACCGCGCGTTCCGGCGGCGGTGTGGGGCGAGCGCGAAGTGCGCGATATGTACGGTTTGATTCCGG
TAGGGCTTCCGGATGAACGTCGTCTGGTGCTGCCGGATGACTGGCCGGATGAACTTTATCCGCTGCGTAAAGACAGCATG
GATTATCGTCAGCGTCCGGCACCGACTACCGATGCTGAAACCTACGAGTTCATCAACGAACTGGGCGACAAGAAAAACAA
TGTCGTGCCGATTGGTCCGCTGCACGTCACTTCTGACGAACCGGGTCACTTCCGTCTGTTCGTCGATGGCGAAAACATTA
TCGACGCCGACTACCGCCTGTTCTATGTCCATCGCGGCATGGAAAAACTGGCGGAAACCCGCATGGGTTACAACGAAGTG
ACCTTCCTCTCTGACCGTGTGTGCGGGATCTGCGGCTTTGCTCACAGCACCGCCTACACCACGTCGGTGGAAAACGCGAT
GGGTATTCAGGTGCCAGAACGTGCGCAGATGATCCGCGCCATTCTGCTGGAGGTGGAACGCCTGCACTCGCATCTGCTCA
ACCTCGGCCTGGCCTGTCACTTTACCGGCTTCGACTCCGGCTTTATGCAGTTCTTCCGCGTGCGTGAAACCTCCATGAAA
ATGGCAGAGATCCTTACCGGTGCACGTAAAACCTACGGCCTGAACCTGATCGGCGGGATTCGTCGCGATCTGCTGAAAGA
CGACATGATCCAGACCCGCCAGCTGGCACAACAGATGCGTCGTGAAGTGCAGGAGCTGGTGGATGTGCTGCTGAGCACAC
CGAACATGGAACAACGCACCGTCGGCATTGGTCGTCTGGACCCGGAAATCGCTCGCGACTTCAGTAACGTTGGCCCGATG
GTCCGTGCCAGCGGTCATGCCCGTGATACCCGTGCCGATCACCCGTTTGTTGGTTATGGCCTGCTGCCAATGGAAGTCCA
CAGCGAGCAGGGCTGCGACGTTATTTCCCGTCTGAAAGTGCGTATCAACGAAGTCTATACCGCGCTGAATATGATCGATT
ACGGTCTGGATAACCTGCCGGGCGGCCCGCTGATGGTGGAAGGCTTTACCTACATTCCGCACCGTTTCGCGCTGGGCTTT
GCCGAAGCGCCGCGCGGCGACGATATCCACTGGAGCATGACCGGCGACAACCAGAAGCTGTACCGCTGGCGCTGCCGTGC
GGCGACCTACGCAAACTGGCCGACCCTGCGCTACATGCTGCGCGGCAACACCGTTTCCGATGCGCCGTTGATTATCGGTA
GCCTGGACCCTTGCTACTCCTGTACCGACCGCATGACCGTGGTCGATGTACGTAAGAAGAAGAGCAAAGTGGTGCCGTAC
AAAGAACTCGAGCGCTACAGCATTGAGCGTAAAAACTCGCCGCTGAAATAA

Upstream 100 bases:

>100_bases
TCGAAAACAGCATGGCCCGTCTGCGTCTTGATATTACTCCGCGCATTACCTGGGCTGGGTTTGGCTTTGCATTTTTAGCG
TTCGTCTCCTTGCTGGCGGC

Downstream 100 bases:

>100_bases
GGAATCGCCATGTTTACCTTTATCAAAAAAGTCATCAAAACCGGCACGGCGACCTCGTCTTATCCGCTGGAGCCGATTGC
GGTTGATAAAAACTTCCGTG

Product: formate hydrogenlyase, subunit E

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MIKESLSMSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVLFGNDERKLNGHYA
VYYVLSMEKGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSM
DYRQRPAPTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEV
TFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMK
MAEILTGARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPM
VRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGF
AEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPY
KELERYSIERKNSPLK

Sequences:

>Translated_576_residues
MIKESLSMSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVLFGNDERKLNGHYA
VYYVLSMEKGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSM
DYRQRPAPTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEV
TFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMK
MAEILTGARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPM
VRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGF
AEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPY
KELERYSIERKNSPLK
>Mature_576_residues
MIKESLSMSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVLFGNDERKLNGHYA
VYYVLSMEKGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSM
DYRQRPAPTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEV
TFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMK
MAEILTGARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPM
VRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGF
AEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPY
KELERYSIERKNSPLK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=387, Percent_Identity=25.5813953488372, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI260898743, Length=377, Percent_Identity=25.4641909814324, Blast_Score=110, Evalue=5e-24,
Organism=Escherichia coli, GI1789076, Length=569, Percent_Identity=100, Blast_Score=1186, Evalue=0.0,
Organism=Escherichia coli, GI1788832, Length=568, Percent_Identity=70.4225352112676, Blast_Score=858, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=563, Percent_Identity=25.7548845470693, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17568379, Length=387, Percent_Identity=25.5813953488372, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17555284, Length=400, Percent_Identity=25, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI221459469, Length=387, Percent_Identity=25.5813953488372, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24638644, Length=387, Percent_Identity=26.6149870801034, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 65770; Mature: 65770

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKESLSMSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQ
CCCCHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCHHHHHHHHHCCC
GGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYPSVTPRVPAAVW
CCEEEEEECCCCCEECCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHC
GEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINE
CCHHHHHHHCCCCCCCCCCCEEECCCCCCHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHH
LGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEV
HCCCCCCEEECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCE
TFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACH
EEHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
FTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMR
ECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
REVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYG
HHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEC
LLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGF
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCHHHHCCC
AEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYS
CCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHC
CTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK
CCCCEEEEEEHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIKESLSMSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQ
CCCCHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCHHHHHHHHHCCC
GGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYPSVTPRVPAAVW
CCEEEEEECCCCCEECCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHC
GEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINE
CCHHHHHHHCCCCCCCCCCCEEECCCCCCHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHH
LGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEV
HCCCCCCEEECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCE
TFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACH
EEHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
FTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMR
ECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
REVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYG
HHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEC
LLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGF
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCHHHHCCC
AEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYS
CCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHC
CTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK
CCCCEEEEEEHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]