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The map label for this gene is istB [H]

Identifier: 170681926

GI number: 170681926

Start: 3230570

End: 3231313

Strand: Reverse

Name: istB [H]

Synonym: EcSMS35_3143

Alternate gene names: 170681926

Gene position: 3231313-3230570 (Counterclockwise)

Preceding gene: 170680910

Following gene: 170682595

Centisome position: 63.75

GC content: 54.57

Gene sequence:

>744_bases
ATGCATGAGCTGGAAGTCCTGCTGAGTCGCCTGAAAATGGAGCATCTGAGTTATCACGTTGAAAGCCTGCTGGAACAGGC
AGCTAAAAAAGAGCTGAACTACCGGGAGTTCCTGTGCATGGCGCTACAGCAGGAATGGAACGGCAGGCATCAGCGCGGTA
TGGAGTCCAGGCTGAAGCAGGCTCGTCTGCCGTGGGTCAAAACGCTGGAGCAGTTCGACTTTACCTTCCAGCCGGGCATC
TACCGTAAGGTTGTCCGGGAACTGGCTGGTCTGGCGTTCGTGGAGCGCAGCGAAAACGTGATCCTGCTGGGACCTCCTGG
TGTCGGAAAAACTCATCTGGCCATAGCTCTTGGCGTGAAAGCGGTGGATGCGGGACATCGGGTACTGTTTATGCCACTGG
ACAGACTGATCGCGACACTGATGAAAGCGAAACAGGAAAACCGGCTGGAGCGTCAGCTACAGCAACTGAGTTATGCCCGG
GTGTTGATCCTGGATGAAATAGGCTATCTGCCGATGAACAGAGAGGATGTCGGAAACGCCTTCATGCGAGGCGTCTCCTC
ACCGATGCGCGTGACTCAAGAAGGGCCTGACGGCTTGTCTCGTTACTGTCCTGTCCGGGTTATCTGTCTGGAGATTCAAC
TCTGTTTCCTCACAGGAGCTCTGTTATGGCAGGTAAAGTTACGGAAACCGCTGTTGTGGGTGGCGTGGATATACATAAAG
ATCTGCACGTTGCCGCTGTCGTAG

Upstream 100 bases:

>100_bases
CGTCTGGCTGGCAGACAGTGCCGGAGCATCACACCCCGCTCTGGCAGCAGGTCAGTCAGGTGGAACATCGACTACTGAGT
GCCTATGAGGAGCTGTTGTG

Downstream 100 bases:

>100_bases
ATCAGAACAATAAAGTTCTGGGGACCCCGTTTTTCTCCACAACACGGCAAGGTTACCGGCAGATGCTGGCATGGATGGCT
TCGTTTGGGGCATTAAAGCG

Product: IS21 transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGI
YRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
VLILDEIGYLPMNREDVGNAFMRGVSSPMRVTQEGPDGLSRYCPVRVICLEIQLCFLTGALLWQVKLRKPLLWVAWIYIK
ICTLPLS

Sequences:

>Translated_247_residues
MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGI
YRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
VLILDEIGYLPMNREDVGNAFMRGVSSPMRVTQEGPDGLSRYCPVRVICLEIQLCFLTGALLWQVKLRKPLLWVAWIYIK
ICTLPLS
>Mature_247_residues
MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGI
YRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
VLILDEIGYLPMNREDVGNAFMRGVSSPMRVTQEGPDGLSRYCPVRVICLEIQLCFLTGALLWQVKLRKPLLWVAWIYIK
ICTLPLS

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 28495; Mature: 28495

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCHHHHHHH
ARLPWVKTLEQFDFTFQPGIYRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGVK
HCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
AVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYARVLILDEIGYLPMNREDVGNA
EECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
FMRGVSSPMRVTQEGPDGLSRYCPVRVICLEIQLCFLTGALLWQVKLRKPLLWVAWIYIK
HHHCCCCCHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
ICTLPLS
HHHCCCC
>Mature Secondary Structure
MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCHHHHHHH
ARLPWVKTLEQFDFTFQPGIYRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGVK
HCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
AVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYARVLILDEIGYLPMNREDVGNA
EECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
FMRGVSSPMRVTQEGPDGLSRYCPVRVICLEIQLCFLTGALLWQVKLRKPLLWVAWIYIK
HHHCCCCCHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
ICTLPLS
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]