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The map label for this gene is psuT [H]

Identifier: 170681919

GI number: 170681919

Start: 2341742

End: 2342992

Strand: Reverse

Name: psuT [H]

Synonym: EcSMS35_2311

Alternate gene names: 170681919

Gene position: 2342992-2341742 (Counterclockwise)

Preceding gene: 170682601

Following gene: 170683370

Centisome position: 46.23

GC content: 50.76

Gene sequence:

>1251_bases
ATGGATATAATGAGAAGTGTTGTGGGGATGGTGGTGTTACTGGCAATAGCATTTTTGTTGTCAGTGAATAAAAAGAGCAT
CAGTTTGCGCACGGTTGGAGCCGCGTTGCTGCTACAAATCGCCATTGGCGGAATCATGCTCTATTTTCCACCAGGAAAAT
GGGCGGTAGAACAGGCGGCATTAGGCGTTCATAAAGTGATGTCTTACAGTGATGCCGGTAGCGCCTTCATTTTTGGTTCG
CTGGTTGGGCCGAAAATGGATGTCCTCTTTGACGGTGCGGGTTTTATCTTCGCCTTTCGCGTACTCCCGGCGATTATCTT
CGTCACTGCGCTCATTAGTCTGCTGTACTACATTGGCGTGATGGGGCTGCTGATTCGCATCCTTGGCAGCATTTTCCAGA
AAGCCCTCAACATCAGCAAAATCGAATCTTTTGTTGCGGTTACCACTATTTTCCTCGGGCAAAATGAGATCCCGGCGATC
GTTAAACCGTTTATCGATCGCATGAATCGCAACGAGTTGTTTACCGCAATTTGTAGCGGGATGGCGTCCATTGCGGGTTC
GATGATGATTGGTTATGCCGGAATGGGCGTACCAATTGACTACCTGTTAGCGGCATCGCTGATGGCGATCCCTGGCGGGA
TTTTGTTTGCACGTATTCTTAGCCCGGCAACCGAGCCTTCGCAGGTCACATTTGAAAATTTATCGTTCAGCGAAACGCCG
CCAAAAAGCTTTATCGAAGCGGCGGCGAGCGGTGCGATGACCGGGCTGAAAATCGCCGCTGGTGTGGCGACGGTGGTAAT
GGTATTTGTGGCAATTATTGCGCTGATCAACGGCATTATCGGCGGAATTGGCGGCTGGTTTGGCTTCGCCAATGCTTCTC
TGGAAAGTATTTTTGGCTATGTGCTGGCACCGCTGGCGTGGATCATGGGTGTGGACTGGAGTGATGCCAACCTTGCGGGT
AGCCTGATTGGGCAGAAACTGGCAATTAACGAATTCGTCGCTTACCTGAGTTTCTCCCCATACCTGCAAACGGGCGGCAC
GCTGGAAGTGAAAACCATTGCGATTATCTCCTTTGCGCTTTGTGGTTTTGCTAACTTTGGTTCTATCGGTGTTGTCGTTG
GGGCATTTTCGGCTATTTCGCCAAAACGCGCACCGGAAATCGCTCAGCTTGGTTTACGGGCGCTGGCAGCAGCAACGCTT
TCCAACCTGATGAGCGCGACCATTGCCGGGTTCTTTATTGGTTTAGCTTGA

Upstream 100 bases:

>100_bases
GGGTTAATTTCTGACGGGCAGAAAGCCGTCGCCCGTACTGATCATACATACCAAAACCTGGCGCTGACGCCGGGTTTACT
GGCATGAAGGGAAAATCATT

Downstream 100 bases:

>100_bases
GTTTCATTGCCGGATGTACGCCTTATTAAGCGTTGCATCCGGCAATCGCGCTATTGCAGATTATTTTTTACTGCATCATC
CCGGAGAATTTATTCTCCGG

Product: NupC family nucleoside transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGS
LVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
PKSFIEAAASGAMTGLKIAAGVATVVMVFVAIIALINGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAG
SLIGQKLAINEFVAYLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATL
SNLMSATIAGFFIGLA

Sequences:

>Translated_416_residues
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGS
LVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
PKSFIEAAASGAMTGLKIAAGVATVVMVFVAIIALINGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAG
SLIGQKLAINEFVAYLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATL
SNLMSATIAGFFIGLA
>Mature_416_residues
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGS
LVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
PKSFIEAAASGAMTGLKIAAGVATVVMVFVAIIALINGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAG
SLIGQKLAINEFVAYLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATL
SNLMSATIAGFFIGLA

Specific function: Could be involved in pseudouridine transport [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI11545853, Length=415, Percent_Identity=33.0120481927711, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI42542381, Length=418, Percent_Identity=32.0574162679426, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI227116277, Length=419, Percent_Identity=29.8329355608592, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI1788488, Length=416, Percent_Identity=99.7596153846154, Blast_Score=807, Evalue=0.0,
Organism=Escherichia coli, GI1788485, Length=416, Percent_Identity=87.9807692307692, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1788737, Length=412, Percent_Identity=30.5825242718447, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17560276, Length=396, Percent_Identity=28.5353535353535, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI71991794, Length=399, Percent_Identity=29.3233082706767, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI25146537, Length=194, Percent_Identity=28.8659793814433, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45552519, Length=408, Percent_Identity=30.6372549019608, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45552517, Length=408, Percent_Identity=30.6372549019608, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI19921868, Length=408, Percent_Identity=30.6372549019608, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI281360430, Length=407, Percent_Identity=30.7125307125307, Blast_Score=192, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011642
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07670 Gate; PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43460; Mature: 43460

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAA
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
LGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGV
HHHHHHHHCCCCCCCEEEHHHCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAIVKPFIDRMNRNELFTAICSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
MASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEECCCCCCCCC
PKSFIEAAASGAMTGLKIAAGVATVVMVFVAIIALINGIIGGIGGWFGFANASLESIFGY
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVAYLSFSPYLQTGGTLEVKTIAIISFAL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEHHHHHHHHHHH
CGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATLSNLMSATIAGFFIGLA
HCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAA
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
LGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGV
HHHHHHHHCCCCCCCEEEHHHCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAIVKPFIDRMNRNELFTAICSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
MASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEECCCCCCCCC
PKSFIEAAASGAMTGLKIAAGVATVVMVFVAIIALINGIIGGIGGWFGFANASLESIFGY
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVAYLSFSPYLQTGGTLEVKTIAIISFAL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEHHHHHHHHHHH
CGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATLSNLMSATIAGFFIGLA
HCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]