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The map label for this gene is rbsK [H]

Identifier: 170681818

GI number: 170681818

Start: 1098979

End: 1100193

Strand: Reverse

Name: rbsK [H]

Synonym: EcSMS35_1084

Alternate gene names: 170681818

Gene position: 1100193-1098979 (Counterclockwise)

Preceding gene: 170682542

Following gene: 170682124

Centisome position: 21.71

GC content: 41.23

Gene sequence:

>1215_bases
ATGAAATTTGAACGCCATCATGAAATATTGAAACGACTCAGTAAATTCGGTTCTGTAAAAGTATCCGATCTGTCCAATAG
CCTCAATGTAACGAAAGAAACTATTCGATCAGATCTTAATGAACTTGCCAGACTTGGCTATCTGACTCGATGTCATGGCG
GTGCTTTTATCGTCCTAGACTCGCTTGATACTATTGCGAAAAATGAAATCGCTTATGCGCTTGAAAATTATGATACAGCC
CAGGGAATAAAAAAAGGCCACTCAACTATGAAAAGTCAAGTATGCGTTATTGGTTCGTTTAATGTTGATATCATCAGTTA
TTTGCCGAGGCTTCCCACTATCGGAGAATCGCTTCTGGCAAGTAATTTTATATTTTCACCGGGGGGAAAAGGTTGTAACC
AAGCGCTGGCAGCAAGCTTTGCCGATACTGATGTTTATTTCATAACAAAAGTAGGTACAGATCACTTTAGTGATTATGCG
ATCAACTTTATGAATTCGTCTAAGATTTATAAAAGTATAATTTATCAAACGAAAGAAACGCAAACCGGAACTGCCACGAT
CCTTGTTAATGAAGGTACTGGCGATAATGTTATTGCGATCTATCCCGGTGCCAACATGACAATGTCGTCCGATGAAATAA
CTATTCAGAAAGAAGCGATTATTAATTCTGATGTCATACTTCTGCAGCTCGAAACAAATTATACCGCTCTGCAACAAGCG
ATCACATTGGCGCAAAAAAACAGTATCCCGGTCATTATTAACCCGGCACCCTATAATGATATCGTCAACGAACTTATTCA
GGATGTTGATTATATCACCCCCAATGAAACAGAAGCTGGTTTACTGTCCGGCATAGACGTACACGATCTTGAATCAGCTA
AACGAGCTGCAGAAGCTATCCACAATAAAGGGGTAAAAAACACGGTTATCACGCTCGGTAGCAAGGGCTCACTCGCATTT
GATGGCAAAAAATTCATTCATTCGCCAGCGTTTCCAGCGGTCGTTAAAAATACGGCAGGAGCCGGGGATGCTTTTAACGG
CGCTCTGGCATCAGGACTTGCGAAAGGAAAATCACTGGAATCAGCACTGTGTTATGCCAGTGCCTTTGCATCCCTGGCGG
TGGAGACAAGCAATGCTTCCGACATGCCTGAACATGAATCTGTGATACATCGAATTCAGAGTATACATTATCAGCAAACT
ATTTTTACGCATTAA

Upstream 100 bases:

>100_bases
AGGCTATTTTTGATTTTTTTTGCTTTTCCCCGCCACTTTATGGTGATTATCACATCAATATACATCGACAGATATTAACG
TAACTGAAGTGAGGTGAAGC

Downstream 100 bases:

>100_bases
GAAGGGACAGAGAATGAATAACACCAAAAAATCACTAAAGGTGCTCTTTATTGGAGAATCCTGGCATATTCATATGATTC
ATTCAAAAGGATATGACAGT

Product: PfkB family DNA-binding protein/kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELARLGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTA
QGIKKGHSTMKSQVCVIGSFNVDIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKEAIINSDVILLQLETNYTALQQA
ITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNETEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAF
DGKKFIHSPAFPAVVKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRIQSIHYQQT
IFTH

Sequences:

>Translated_404_residues
MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELARLGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTA
QGIKKGHSTMKSQVCVIGSFNVDIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKEAIINSDVILLQLETNYTALQQA
ITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNETEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAF
DGKKFIHSPAFPAVVKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRIQSIHYQQT
IFTH
>Mature_404_residues
MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELARLGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTA
QGIKKGHSTMKSQVCVIGSFNVDIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKEAIINSDVILLQLETNYTALQQA
ITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNETEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAF
DGKKFIHSPAFPAVVKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRIQSIHYQQT
IFTH

Specific function: Ribose metabolism; first step. [C]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=285, Percent_Identity=32.280701754386, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790193, Length=266, Percent_Identity=38.3458646616541, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI87082343, Length=280, Percent_Identity=26.7857142857143, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788491, Length=304, Percent_Identity=25.6578947368421, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788017, Length=258, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17532905, Length=300, Percent_Identity=35, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319884, Length=330, Percent_Identity=28.1818181818182, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20128807, Length=296, Percent_Identity=35.472972972973, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24583886, Length=276, Percent_Identity=30.7971014492754, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR011877
- InterPro:   IPR002139 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.15 [H]

Molecular weight: Translated: 43666; Mature: 43666

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS51000 HTH_DEOR_2 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELARLGYLTRCHGGAFIVLD
CCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEE
SLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNVDIISYLPRLPTIGESLLA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCHHHHHH
SNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYAINFMNSSKIYKSIIYQTKET
CCCEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCC
QTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKEAIINSDVILLQLETNYTALQQA
CCCEEEEEEECCCCCCEEEEECCCCCEECCCCEEEEHHHCCCCCEEEEEEECCHHHHHHH
ITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNETEAGLLSGIDVHDLESAKRAAEAI
HHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHH
HNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAVVKNTAGAGDAFNGALASGLAKGKSLE
HHCCCCCEEEEECCCCCEEECCHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCHH
SALCYASAFASLAVETSNASDMPEHESVIHRIQSIHYQQTIFTH
HHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELARLGYLTRCHGGAFIVLD
CCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEE
SLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNVDIISYLPRLPTIGESLLA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCHHHHHH
SNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYAINFMNSSKIYKSIIYQTKET
CCCEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCC
QTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKEAIINSDVILLQLETNYTALQQA
CCCEEEEEEECCCCCCEEEEECCCCCEECCCCEEEEHHHCCCCCEEEEEEECCHHHHHHH
ITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNETEAGLLSGIDVHDLESAKRAAEAI
HHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHH
HNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAVVKNTAGAGDAFNGALASGLAKGKSLE
HHCCCCCEEEEECCCCCEEECCHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCHH
SALCYASAFASLAVETSNASDMPEHESVIHRIQSIHYQQTIFTH
HHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]