Click here to switch to the map view.

The map label for this gene is frvB [H]

Identifier: 170681696

GI number: 170681696

Start: 4392100

End: 4393557

Strand: Reverse

Name: frvB [H]

Synonym: EcSMS35_4289

Alternate gene names: 170681696

Gene position: 4393557-4392100 (Counterclockwise)

Preceding gene: 170682227

Following gene: 170683436

Centisome position: 86.69

GC content: 55.56

Gene sequence:

>1458_bases
GTGGCGATGGAGTCATCCTTACGTATTGTCGCGATCACCAACTGCCCCGCCGGGATCGCTCACACCTACATGGTGGCGGA
AGCCCTGGAACAGAAAGCCCGTTCTCTCGGTCATACCATTAAAGTTGAAACTCAAGGATCAAGCGGCATCGAAAACCGCT
TATCCAGCGAAGAGATTGCCGCTGCCGATTACGTGATTCTCGCTACCGGGCGTGGCCTGAGCGGTGATGATCGCGCGCGA
TTTGCCGGGAAAAAGGTTTATGAGATTGCCATCTCCCAGGCGCTGAAAAATATCGACCAGATTTTCAGTGAATTACCGAC
AAACTCGCAGCTTTTTGCCGCAGATAGCGGCGTGAAGCTCGGCAAACAGGAAGTGCAAAGTGGCAGCGTAATGAGCCATC
TGATGGCGGGTGTTTCTGCGGCGCTGCCGTTTGTCATCGGTGGCGGTATCCTGGTGGCGCTTGCCAACATGCTGGTGCAG
TTCGGTTTGCCGTATACCGATATGTCGAAAGGCGCGCCGTCGTTTACCTGGGTGGTGGAATCCATCGGTTATCTCGGCTT
CACCTTTATGATCCCCATCATGGGCGCTTATATTGCCTCGTCGATTGCCGATAAACCGGCTTTCGCTCCGGCGTTTCTGG
TTTGCTATCTGGCGAATGACAAAGCCCTGCTCGGCACCCAGTCTGGCGCGGGCTTCCTCGGTGCGGTGGTGCTGGGGCTG
GCGATTGGCTATTTCGTCTTCTGGTTCCGTAAAGTGCGCCTTGGCAAAGCATTACAACCGCTGCTCGGTTCCATGCTGAT
CCCGTTCGTTACTCTGCTGGTTTTTGGCGTACTGACTTACTACGTTATCGGACCGGTGATGTCCGACCTCATGGGCGGGT
TGCTGCACTTCCTGAACACCATTCCTCCGTCAATGAAGTTTGCGGCGGCGTTTCTGGTTGGTGCGATGCTGGCATTCGAT
ATGGGCGGCCCCATCAACAAAACCGCCTGGTTCTTCTGCTTCTCTCTGCTGGAAAAACACATTTACGACTGGTACGCCAT
CGTCGGCGTTGTCGCGCTGATGCCGCCTGTCGCGGCGGGTCTTGCGACGTTCATTGCGCCCAAACTGTTTACTCAACAGG
AAAAAGAAGCCGCCAGTAGCGCCATTGTGGTCGGTGCCACGGTCGCGACCGAACCGGCTATTCCTTACGCTCTGGCGGCA
CCACTGCCAATGATCACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGACT
GGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTAGGTTCGTTTTACCTGGTGCTGGCGATTG
GCCTCGCGCTGAACATTTCATTCATTATCGTCCTGAAAGGATTATGGTTACGCCGCAAAGCCAAAGCTGCGCAACAGGAG
CTTGTCCATGAACATTGA

Upstream 100 bases:

>100_bases
TGCCGCAAAATTATCCACCAGGATTTTATCAACCAACTGCGACAGGGCGACGCCGACCAGGTACTTGCTTTGTTAAATCA
AACCCTCAGCTCATAAGGAA

Downstream 100 bases:

>100_bases
GTTGCTGCAACAGTTGTGCGAAGCCAGCGCCGTCAGCGGCGATGAACAGGAAGTTCGCGACATTCTGATAAACACGCTGG
AACCTTGCGTTAATGAGATC

Product: putative PTS system transporter subunits IIBC

Products: NA

Alternate protein names: Fructose-like phosphotransferase enzyme IIB component; PTS system fructose-like EIIB component; Fructose-like permease IIC component; PTS system fructose-like EIIC component [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MAMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGIENRLSSEEIAAADYVILATGRGLSGDDRAR
FAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQ
FGLPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGL
AIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFD
MGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTQQEKEAASSAIVVGATVATEPAIPYALAA
PLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQE
LVHEH

Sequences:

>Translated_485_residues
MAMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGIENRLSSEEIAAADYVILATGRGLSGDDRAR
FAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQ
FGLPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGL
AIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFD
MGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTQQEKEAASSAIVVGATVATEPAIPYALAA
PLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQE
LVHEH
>Mature_484_residues
AMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGIENRLSSEEIAAADYVILATGRGLSGDDRARF
AGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQF
GLPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLA
IGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDM
GGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTQQEKEAASSAIVVGATVATEPAIPYALAAP
LPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQEL
VHEH

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI87082348, Length=483, Percent_Identity=99.5859213250518, Blast_Score=963, Evalue=0.0,
Organism=Escherichia coli, GI1788492, Length=444, Percent_Identity=34.009009009009, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1786951, Length=479, Percent_Identity=32.776617954071, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1790386, Length=291, Percent_Identity=35.7388316151203, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=50, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788729, Length=322, Percent_Identity=27.3291925465839, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788730, Length=100, Percent_Identity=41, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790390, Length=70, Percent_Identity=45.7142857142857, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51309; Mature: 51178

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGIENRLSSEEIA
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHH
AADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKL
HCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHH
GKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFGLPYTDMSKGAPSFTWVVE
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
SIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCEEEECCCCCHHHHHHHHHH
AIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAG
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATFIAPKLFTQQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVI
HHHHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHH
AFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVHEH
HHCCC
>Mature Secondary Structure 
AMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGIENRLSSEEIA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHH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HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503; 8019415 [H]