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The map label for this gene is ygeV [H]

Identifier: 170681642

GI number: 170681642

Start: 3082988

End: 3084766

Strand: Reverse

Name: ygeV [H]

Synonym: EcSMS35_3002

Alternate gene names: 170681642

Gene position: 3084766-3082988 (Counterclockwise)

Preceding gene: 170680222

Following gene: 170683156

Centisome position: 60.86

GC content: 46.04

Gene sequence:

>1779_bases
ATGGAGCTTGCTACTACGCAGTCAGTATTGATGCAAATTCAACCGACAATTCAGCGTTTTGCCAGAATGCTTGCCAGCGT
TTTGCAGCTTGAGGTTGAGATCGTTGATGAAAACTTGTGTCGCGTCGCCGGAACGGGCGCGTATGGGAAGTTTCTTGGTC
GCCAGTTGAGCGGCAACTCACGCCTGCTCCGCCACGTCCTGGAAACGAAAACTGAAAAAGTTGTGACACAGTCTCGCTTC
GATCCCCTTTGCGAAGGTTGCGATAGTAAAGAAAATTGCCGCGAAAAAGCATTTCTGGGTACGCCTGTCATTTTACAGGA
TCGTTGTGTTGGGGTGATAAGTTTGATTGCCGTTACCCACGAGCAACAAGAGCATATTAGTGATAATTTACGCGAATTTT
CTGATTACGTTCGCCATATATCCACCATTTTTGTTTCGAAACTTCTGGAGGATCAGGGACCAGGAGATAACATCAGTAAA
ATATTCGCGACCATGATCGATAATATGGATCAGGGTGTATTAGTTGTTGATGACGATAATCGGATTCAGTTTGTTAATCA
GACTGCCTTAAAAACACTTGGTGTTGTACAAAATAATATTATTGGGAAACCTATCCGTTTCAGACCATTAACATTTGAGA
GTAATTTTACCCATGGACATATGCAGCATATTGTTTCGTGGGACGATAAAAGTGAATTAATCATTGGTCAATTGCATAAC
ATTCAGGGCCGACAATTATTTTTAATGGCGTTTCACCAATCGCATACCAGTTTTTCTGTAGCAAATGCACCTGATGAACC
GCATATTGAACAATTGGTTGGCGAGTGCCGTGTTATGCGGCAATTAAAACGACTCATTAGCCGTATTGCACCCAGCCCAT
CCAGCGTTATGGTGGTTGGTGAAAGCGGCACGGGTAAAGAAGTTGTCGCCCGTGCAATCCATAAGTTGAGCGGAAGACGG
AATAAACCCTTTATTGCTATCAACTGTGCCGCGATTCCGGAGCAGCTTCTGGAGAGCGAACTGTTCGGTTATGTTAAAGG
CGCATTTACTGGCGCTTCTGCCAACGGTAAAACAGGGTTGATTCAGGCGGCGAATACGGGCACGCTGTTTCTCGATGAAA
TAGGTGATATGCCATTAATGTTGCAGGCTAAATTACTGCGCGCTATTGAGGCGCGTGAAATTCTGCCGATTGGTGCCAGT
AGCCCAATACAAGTCGACATTCGCATCATTTCTGCAACTAACCAGAATTTGGCCCAGTTCATTGCCGAAGGTAAATTCCG
CGAAGATCTCTTCTACCGACTTAATGTTATCCCGATAACTCTGCCACCGCTGCGTGAACGTCAGGAAGATATTGAACTAC
TGGTGCATTACTTTTTACATCTGCATACCCGTCGTCTGGGATCGGTTTATCCGGGCATTGCTCCCGATGTCGTCGAAATA
TTGCGTAAGCATCGTTGGCCCGGAAACCTGCGCGAGTTAAGCAATTTGATGGAATATCTGGTTAACGTGGTTCCTTCAGG
TGAAGTTATCGACAGCACGCTATTGCCGCCAAATCTGCTGAATAATGGCACAACGGAGCAAAGTGATGTAACAGAGGTCA
ACGAGGCGCACCTGGCACTCGATGATGCGGGCGGCACGGCGCTGGAGGAGATGGAAAAGCAAATGATCCGCGAGGCGCTT
TCACGTCATAACAGCAAGAAGCAAGTTGCTGATGAACTGGGCATCGGCATTGCTACGCTCTATCGCAAGATTAAGAAATA
TGAGTTGTTAAACACATAA

Upstream 100 bases:

>100_bases
TTTTATCAAAGCCAATTTCGCCTGAGTTAATATCATCATGATCTGTGAACCATCAACGTCTTCAATGATCGACTTTTAAG
TAAGTCTGGAAGGTAAAGCT

Downstream 100 bases:

>100_bases
CCTCAGTGAATCCGGCCTGAATTCAGGCCGGATATCCTTTTACTTCGTTTTCTCGCAATCCAGAACTGTATTTACAATCA
TCTGATACCCCGTGCAGCGA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAGTGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRF
DPLCEGCDSKENCREKAFLGTPVILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
IFATMIDNMDQGVLVVDDDNRIQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGHMQHIVSWDDKSELIIGQLHN
IQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRR
NKPFIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREILPIGAS
SPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEI
LRKHRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVNEAHLALDDAGGTALEEMEKQMIREAL
SRHNSKKQVADELGIGIATLYRKIKKYELLNT

Sequences:

>Translated_592_residues
MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAGTGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRF
DPLCEGCDSKENCREKAFLGTPVILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
IFATMIDNMDQGVLVVDDDNRIQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGHMQHIVSWDDKSELIIGQLHN
IQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRR
NKPFIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREILPIGAS
SPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEI
LRKHRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVNEAHLALDDAGGTALEEMEKQMIREAL
SRHNSKKQVADELGIGIATLYRKIKKYELLNT
>Mature_592_residues
MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAGTGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRF
DPLCEGCDSKENCREKAFLGTPVILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
IFATMIDNMDQGVLVVDDDNRIQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGHMQHIVSWDDKSELIIGQLHN
IQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRR
NKPFIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREILPIGAS
SPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEI
LRKHRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVNEAHLALDDAGGTALEEMEKQMIREAL
SRHNSKKQVADELGIGIATLYRKIKKYELLNT

Specific function: Unknown

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=592, Percent_Identity=99.3243243243243, Blast_Score=1212, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=306, Percent_Identity=48.0392156862745, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1790299, Length=329, Percent_Identity=43.4650455927052, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082117, Length=328, Percent_Identity=42.3780487804878, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1790437, Length=318, Percent_Identity=42.4528301886792, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788905, Length=252, Percent_Identity=48.4126984126984, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1789087, Length=321, Percent_Identity=42.3676012461059, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1786524, Length=330, Percent_Identity=39.0909090909091, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87082152, Length=322, Percent_Identity=38.8198757763975, Blast_Score=212, Evalue=7e-56,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=44.8275862068966, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1787583, Length=349, Percent_Identity=32.9512893982808, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1789828, Length=264, Percent_Identity=35.6060606060606, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081858, Length=450, Percent_Identity=28.6666666666667, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 66108; Mature: 66108

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAGTGAYGKFLGRQLSGNS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
RLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTPVILQDRCVGVISLIAVTH
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISKIFATMIDNMDQGVLVVDDDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
RIQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGHMQHIVSWDDKSELIIGQLHN
CEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCEEHHHHCC
IQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVG
CCCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGL
CCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREILPIGASSPIQVDIRIISATNQNLAQF
EEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCHHHHHH
IAEGKFREDLFYRLNVIPITLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEI
HHCCCHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LRKHRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVNEAHLAL
HHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHCCCCCCCCHHHHHHHHHEEE
DDAGGTALEEMEKQMIREALSRHNSKKQVADELGIGIATLYRKIKKYELLNT
ECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAGTGAYGKFLGRQLSGNS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
RLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTPVILQDRCVGVISLIAVTH
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISKIFATMIDNMDQGVLVVDDDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
RIQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGHMQHIVSWDDKSELIIGQLHN
CEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCEEHHHHCC
IQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVG
CCCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGL
CCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREILPIGASSPIQVDIRIISATNQNLAQF
EEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCHHHHHH
IAEGKFREDLFYRLNVIPITLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEI
HHCCCHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LRKHRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVNEAHLAL
HHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHCCCCCCCCHHHHHHHHHEEE
DDAGGTALEEMEKQMIREALSRHNSKKQVADELGIGIATLYRKIKKYELLNT
ECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]