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The map label for this gene is aegA1 [H]

Identifier: 170681460

GI number: 170681460

Start: 2667855

End: 2669834

Strand: Reverse

Name: aegA1 [H]

Synonym: EcSMS35_2614

Alternate gene names: 170681460

Gene position: 2669834-2667855 (Counterclockwise)

Preceding gene: 170683913

Following gene: 170680976

Centisome position: 52.68

GC content: 55.51

Gene sequence:

>1980_bases
ATGAATCGTTTTATTATGGCCAACAGTCAGCAATGTCTGGGTTGTCATGCTTGTGAAATCGCCTGTGTCATGGCTCACAA
TGACGAGCAACATGTCCTGAGCCAACGCCATTTTCATCCCCGAATTACGGTTATCAAACATCAGCAGCAACGTAGTGCAG
TGACCTGTCACCATTGTGAAGATGCGCCCTGCGCCCGTAGCTGCCCTAATGGCGCAATCAGCCACGTTGATGACAGCATT
CAGGTCAATCAGCAAAAGTGTATTGGCTGTAAATCCTGCGTGGTGGCCTGTCCATTTGGTACGATGCAAATCGTCCTGAC
ACCCGTTGCGGCTGGCAAAGTTAAAGCCACGGCGCATAAATGCGACCTTTGTGCAGGGCGCGAAAACGGTCCTGCCTGTG
TCGAAAATTGCCCGGCGGACGCGCTGCAACTGGTCACTGACGCCGCACTCTCCGGCATGGCGAAATCCCGCCGCTTACGC
ACCGCACGTCAGGAACATCAACCGTGGCATGCCAGTACCGCGGCACAAGAAATGCCGGTAATGAGTAAAGTCGAGCAAAT
GCAGGCAACGCCCGCGCGTGGCGAGCCGGACAAACTGGCGATTGAAGCGCGCAAAACCGGTTTTGATGAAATTTATCTGC
CATTCCGTGCCGATCAGGCACAACGGGAAGCCTCGCGTTGCCTTAAGTGTGGCGAGCACAGCGTTTGTGAATGGACCTGC
CCGCTGCATAACCATATACCGCAGTGGATTGAACTGGTGAAAGCAGGAAACATCGACGCCGCCGTCGAGCTTTCTCACCA
GACCAACACCCTGCCGGAAATTACCGGACGCGTTTGCCCGCAAGACCGTTTGTGTGAAGGTGCCTGTACTATTCGCGATG
AGCACGGCGCAGTAACTATCGGCAACATTGAACGCTACATTTCAGATCAGGCGTTGGCGAAAGGTTGGCGTCCTGACTTA
AGTCATGTCACCAAAGTGGACAAGCGAGTGGCGATTATCGGTGCGGGTCCGGCAGGGCTGGCCTGTGCGGATGTTCTGAC
CCGCAATGGCGTAGAGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCA
AACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAGTG
GGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAA
AGCGGGTTTACCCAATGAAGATGCGCCGGGCGTTTATGACGCCCTGCCGTTCCTCATCGCCAACACTAAACAGGTGATGG
GACTCGAAGAGCTACCGGAAGAGCCGTTTATCAATACCGCCGGACTCAACGTCGTGGTACTGGGCGGCGGCGACACCGCG
ATGGACTGTGTGCGTACCGCGCTGCGCCACGGCGCGAGTAACGTCACCTGCGCTTATCGTCGTGATGAAGCTAACATGCC
AGGCTCGAAGAAAGAGGTGAAGAACGCCCGCGAAGAGGGGGCCAACTTTGAATTTAACGTCCAGCCGGTGGCGCTTGAGC
TGAATGAGCAAGGTCACGTTTGCGGAATTCGTTTCCTGCGCACGCGTCTTGGCGAGCCGGATGCCCAGGGGCGTCGGCGT
CCAGTGCCGGTGGAAGGCAGTGAATTTGTCATGCCAGCCGACGCGGTGATTATGGCGTTTGGCTTCAATCCGCACGGGAT
GCCGTGGCTGGAGTCGCACGGTGTAACGGTAGACAAATGGGGTCGCATCATCGCGGATGTGGAAAGCCAGTACCGTTACC
AGACCACCAATCCGAAAATCTTCGCTGGTGGTGACGCCGTGCGTGGTGCGGATCTGGTGGTTACCGCAATGGCAGAAGGA
CGTCATGCGGCACAGGGGATTATTGACTGGCTGGGGGTAAAATCAGTCAAATCTCACTGA

Upstream 100 bases:

>100_bases
TTATTCTGGGCACATGAACATTGCCGATTCCCCTGTAAGTCGGGTAATAACAACACTCATATAAAGAATAAGGTTTTTAC
AACCAAAAAAGAAGGTCGTT

Downstream 100 bases:

>100_bases
TAGCCTGCGCAGACAAACCCGACTTCACAGCGTAAGATAATTGTTCATTTCGCGCTGTGGAGTCGGTATGACGCAACAAA
TCACCCTCATTAAAGACAAA

Product: putative oxidoreductase Fe-S binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITVIKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSI
QVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDL
SHVTKVDKRVAIIGAGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEV
GKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRR
PVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEG
RHAAQGIIDWLGVKSVKSH

Sequences:

>Translated_659_residues
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITVIKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSI
QVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDL
SHVTKVDKRVAIIGAGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEV
GKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRR
PVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEG
RHAAQGIIDWLGVKSVKSH
>Mature_659_residues
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITVIKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSI
QVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDL
SHVTKVDKRVAIIGAGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEV
GKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRR
PVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEG
RHAAQGIIDWLGVKSVKSH

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=493, Percent_Identity=29.2089249492901, Blast_Score=164, Evalue=3e-40,
Organism=Escherichia coli, GI1788811, Length=659, Percent_Identity=99.5447647951442, Blast_Score=1362, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=656, Percent_Identity=54.5731707317073, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1789606, Length=461, Percent_Identity=66.8112798264642, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=32.0712694877506, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1789067, Length=172, Percent_Identity=48.8372093023256, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789243, Length=433, Percent_Identity=33.4872979214781, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI2367245, Length=147, Percent_Identity=52.3809523809524, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI87082179, Length=166, Percent_Identity=44.578313253012, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789079, Length=185, Percent_Identity=38.3783783783784, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87082114, Length=188, Percent_Identity=37.7659574468085, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787122, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787872, Length=153, Percent_Identity=30.718954248366, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI2367345, Length=161, Percent_Identity=33.5403726708075, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787749, Length=187, Percent_Identity=25.1336898395722, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790326, Length=168, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI226510944, Length=148, Percent_Identity=33.7837837837838, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17570289, Length=480, Percent_Identity=33.5416666666667, Blast_Score=209, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI71984108, Length=446, Percent_Identity=32.5112107623318, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=469, Percent_Identity=33.0490405117271, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI28574881, Length=471, Percent_Identity=36.3057324840764, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24665539, Length=471, Percent_Identity=36.3057324840764, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24665547, Length=471, Percent_Identity=36.3057324840764, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24665543, Length=471, Percent_Identity=36.3057324840764, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24640763, Length=440, Percent_Identity=28.8636363636364, Blast_Score=152, Evalue=1e-36,
Organism=Drosophila melanogaster, GI18858217, Length=440, Percent_Identity=28.8636363636364, Blast_Score=152, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 71908; Mature: 71908

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00028 ZINC_FINGER_C2H2_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITVIKHQQQRSAVTCHHCE
CCCEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEEEEHHHCCCEEEECCC
DAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHK
CCCCHHCCCCCCHHHCCCCCEECHHHHCCCCHHEEECCCCCEEEEEEECCCCCEEECHHH
CDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLRTARQEHQPWHASTAAQEMPV
HHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHH
MSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
HHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTI
CHHHCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCHHHHCCCCCEEEECCCEEEE
GNIERYISDQALAKGWRPDLSHVTKVDKRVAIIGAGPAGLACADVLTRNGVEVTVYDRHP
CCHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCC
EIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEVGKDVSLDSLLEQYDAVFVGV
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCEEEEEC
GTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
CCHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHCCCCCCEECCCCEEEEECCCCHH
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHV
HHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCC
CGIRFLRTRLGEPDAQGRRRPVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKW
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCHHCCCCCHHHH
GRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH
HHHHHHHHHHCEEECCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITVIKHQQQRSAVTCHHCE
CCCEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEEEEHHHCCCEEEECCC
DAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHK
CCCCHHCCCCCCHHHCCCCCEECHHHHCCCCHHEEECCCCCEEEEEEECCCCCEEECHHH
CDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLRTARQEHQPWHASTAAQEMPV
HHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHH
MSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
HHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTI
CHHHCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCHHHHCCCCCEEEECCCEEEE
GNIERYISDQALAKGWRPDLSHVTKVDKRVAIIGAGPAGLACADVLTRNGVEVTVYDRHP
CCHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCC
EIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEVGKDVSLDSLLEQYDAVFVGV
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCEEEEEC
GTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
CCHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHCCCCCCEECCCCEEEEECCCCHH
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHV
HHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCC
CGIRFLRTRLGEPDAQGRRRPVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKW
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCHHCCCCCHHHH
GRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH
HHHHHHHHHHCEEECCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]