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The map label for this gene is insH10 [H]

Identifier: 170681327

GI number: 170681327

Start: 656167

End: 657159

Strand: Reverse

Name: insH10 [H]

Synonym: EcSMS35_0642

Alternate gene names: 170681327

Gene position: 657159-656167 (Counterclockwise)

Preceding gene: 170684133

Following gene: 170680591

Centisome position: 12.97

GC content: 55.19

Gene sequence:

>993_bases
ATGAGCCATCAACTCACCTTCGCCGATAGTGAATTCAGCACTAAGCGCCGTCAGACCCGAAAAGAGATTTTCCTCTCCCG
CATGGAGCAGATTCTGCCATGGCAGAATATGACCGCTGTCATCGAGCCGTTTTATCCCAAGGCGGGCAATGGCCGACGGC
CCTATCCGCTGGAGACCATGCTGCGTATTCACTGCATGCAGCATTGGTACAACCTGAGCGACGGTGCCATGGAAGATGCC
CTGTACGAAATCGCCTCCATGCGCCTGTTTGCCCGATTATCCCTGGATAGCGCCCTGCCGGATCGCACCACCATCATGAA
TTTCCGCCACCTGCTCGAGCAGCATCAACTGGCCCGTCAATTGTTCAAGACCATCAATCGCTGGCTGGCCGAAGCAGGCG
TCATGATGACCCAAGGCACTTTGGTGGATGCCACCATCATTGAGGCACCCAGCTCTACCAAGAACAAAGAGCAGCAACGC
GATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACATTGGTGTCGATGCCAAGAG
TGGCCTGACCCACAGCCTAGTCACCACCGCGGCCAACGAGCATGACCTCAATCAGCTGGGTAATCTGCTTCATGGTGAGG
AGCAATTTGTCTCAGCCGATGCCGGCTACCAAGGAGCGCCACAGCGCGAGGAGCTGGCCGAGGTGGATGTGGACTGGCTG
ATCGCCGAGCGTCCCGGCAAGGTAAAAACCTTGAAGCAGCATCCGCGCAAGAACAAAACGGCCATCAACATCGAATACAT
GAAAGCCAGCATCCGTGCCAGGGTGGAGCACCCGTTTCGCATCATCAAGCGGCAGTTCGGCTTCGTGAAAGCCAGATACA
AGGGGCTGCTGAAAAACGATAACCAACTGGCGATGTTATTCACCCTGGCCAACCTGTTTCGGGTGGACCAAATGATACGT
CAGGGGAGAGATCTCAGTAAAAACCGGAAATAA

Upstream 100 bases:

>100_bases
TCTAAACGTTGTCATCCACTCATCAGCGTTAGGGAAGGTGCGAACAAGTTCCTGATATGAGATCATCATATTCATCCGGA
GCGCATCCCAGAGGGACATC

Downstream 100 bases:

>100_bases
CGCCAGAAATGGTGGAAAAAATAGCCTAAATAGGCTGATTCGATGTGTTTGCGGGAAAAAAATCGGCCCAGATCCGCGAA
ATTTTAATCAGCGAGTCTGC

Product: IS5 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDA
LYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWL
IAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIR
QGRDLSKNRK

Sequences:

>Translated_330_residues
MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDA
LYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWL
IAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIR
QGRDLSKNRK
>Mature_329_residues
SHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDAL
YEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRD
PEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLI
AERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQ
GRDLSKNRK

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=321, Percent_Identity=99.6884735202492, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1789921, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1789612, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1789355, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1788518, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1788342, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1787592, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1786877, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1786764, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1786453, Length=321, Percent_Identity=98.4423676012461, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1788303, Length=321, Percent_Identity=98.1308411214953, Blast_Score=662, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 38169; Mature: 38038

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFYPKAGNGRRPYPLETM
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHH
LRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPEMHQTKKGNQWHFGMKAH
HHHHHHHHHHHCCCEEECCCEEEHHEEECCCCCCCCHHHCCCHHHHCCCCCCEECCEEEE
IGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWL
ECCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHCCHHEE
IAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKND
EECCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NQLAMLFTLANLFRVDQMIRQGRDLSKNRK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFYPKAGNGRRPYPLETM
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHH
LRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPEMHQTKKGNQWHFGMKAH
HHHHHHHHHHHCCCEEECCCEEEHHEEECCCCCCCCHHHCCCHHHHCCCCCCEECCEEEE
IGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWL
ECCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHCCHHEE
IAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKND
EECCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NQLAMLFTLANLFRVDQMIRQGRDLSKNRK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]