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The map label for this gene is hcaR [H]

Identifier: 170681286

GI number: 170681286

Start: 2763340

End: 2764233

Strand: Reverse

Name: hcaR [H]

Synonym: EcSMS35_2690

Alternate gene names: 170681286

Gene position: 2764233-2763340 (Counterclockwise)

Preceding gene: 170682346

Following gene: 170684196

Centisome position: 54.54

GC content: 50.0

Gene sequence:

>894_bases
GTGATGGAACTACGGCATTTACGCTATTTCGTCGCAGTGGCGCAGGCACTGAACTTTACCCGTGCGGCGGAAAAACTGCA
TACCTCACAGCCTTCGTTAAGCAGCCAGATCCGCGATCTTGAAAACTGTGTCGGTGTTCCGTTACTGGTGAGGGATAAGC
GCAAAGTCGCGTTGACGGCGGCGGGAGAATGTTTTCTCCAGGACGCGCTGGCAATCCTCGAACAAGCGGAAAATGCCAAA
TTACGGGCGCGGAAAATTGTTCAGGAAGACAGACAATTAACCATTGGCTTTGTGCCATCGGCGGAAGTGAATTTACTGCC
AAAAGTATTACCGATGTTTCGTCTCAGACAGCCAGACACCTTAATTGAGCTGGTGAGTTTAATCACCACGGAACAGGAAG
AAAAAATTCGCCGTGGTGAACTCGACGTCGGCTTAATGCGCCATCCCGTTTATAGCCCGGAGATTGATTATCTGGAGCTT
TTTGACGAACCATTAGTGGTGGTGTTACCGGTTGATCACCCTTTAGCACATGAAAAAGAGATCACTGCCGCGCAACTGGA
TGGCGTGAATTTCGTCAGTACTGATCCAGCGTATTCCGGTTCGCTTGCGCCGATCGTTAAAGCGTGGTTTGCGCAAGAAA
ACAGCCAGCCAAATATCGTCCAGGTGGCAACGAATATTCTGGTGACCATGAATCTGGTGGGGATGGGGCTGGGCGTCACT
TTGATACCCGGTTATATGAATAATTTTAATACCGGCCAGGTGGTATTTCGACCCATTGCCGGAAATGTGCCTGCCATTGC
TTTACTGATGGCGTGGAAGAAAGGGGAGATGAAGCCGGCATTACGCGATTTCATCGCCATTGTGCAGGAACGTTTGGCAA
GCGTAACGGCATAA

Upstream 100 bases:

>100_bases
CCTTCTATTTTTGTGCCATAAGGATGTTGCTGGTTGGTTGCTGATATGTGAAATAGTTAATTTCTACCAGCTGAGACGTT
TTTTCGATAACAAGGGGAAG

Downstream 100 bases:

>100_bases
TTACGCATTATTTTTGCAACATGCGTCGCGGATCGCAGTTTTTTAGTGCTTGTGAACTGGTGTTACAGACATCGGGAAGC
AGGCTCGAATATTCTTATTT

Product: DNA-binding transcriptional regulator HcaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MMELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAAGECFLQDALAILEQAENAK
LRARKIVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLEL
FDEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVT
LIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEMKPALRDFIAIVQERLASVTA

Sequences:

>Translated_297_residues
MMELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAAGECFLQDALAILEQAENAK
LRARKIVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLEL
FDEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVT
LIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEMKPALRDFIAIVQERLASVTA
>Mature_297_residues
MMELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAAGECFLQDALAILEQAENAK
LRARKIVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLEL
FDEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVT
LIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEMKPALRDFIAIVQERLASVTA

Specific function: Transcriptional activator of the hca operon for 3- phenylpropionic acid catabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=98.9864864864865, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1787879, Length=286, Percent_Identity=36.7132867132867, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI157672245, Length=249, Percent_Identity=31.3253012048193, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI2367136, Length=286, Percent_Identity=29.020979020979, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=30.7377049180328, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=25.9259259259259, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87081904, Length=182, Percent_Identity=34.6153846153846, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=29.7520661157025, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=25.5972696245734, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=28.9156626506024, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790262, Length=287, Percent_Identity=26.8292682926829, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788297, Length=175, Percent_Identity=33.1428571428571, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787530, Length=245, Percent_Identity=28.1632653061224, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787601, Length=284, Percent_Identity=25.7042253521127, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=300, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788296, Length=235, Percent_Identity=28.5106382978723, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789173, Length=176, Percent_Identity=34.0909090909091, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32927; Mature: 32927

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCEEEEEH
AGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCHH
LIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELFDEPLVVVLPVDHPLAHEKE
HHHHHHHHHCCHHHHHHHCCCCHHHHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCC
ITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVT
CHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
LIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEMKPALRDFIAIVQERLASVTA
ECCHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCEEEEEH
AGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEVNLLPKVLPMFRLRQPDT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCHH
LIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELFDEPLVVVLPVDHPLAHEKE
HHHHHHHHHCCHHHHHHHCCCCHHHHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCC
ITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNIVQVATNILVTMNLVGMGLGVT
CHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
LIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEMKPALRDFIAIVQERLASVTA
ECCHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 9603882 [H]