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The map label for this gene is gspD [H]

Identifier: 170681179

GI number: 170681179

Start: 3318117

End: 3320177

Strand: Reverse

Name: gspD [H]

Synonym: EcSMS35_3247

Alternate gene names: 170681179

Gene position: 3320177-3318117 (Counterclockwise)

Preceding gene: 170681120

Following gene: 170683342

Centisome position: 65.51

GC content: 54.73

Gene sequence:

>2061_bases
GTGTTTTGGCGTGATATGACGTTGTCGGTCTGGCGTAAGAAGACAACTGGCCTCAAAACAAAAAAGCGTTTACTGCCGCT
GGTGCTGGCAGCGGCATTATGCAGTTCACCGGTCTGGGCGGAAGAAGCCACTTTCACCGCTAATTTTAAAGATACCGACC
TGAAATCGTTCATCGAAACCGTCGGCGCTAACCTTAATAAAACCATCATTATGGGGCCGGGCGTTCAGGGGAAAGTGAGT
ATTCGCACTATGACTCCACTCAATGAACGCCAGTATTACCAGCTATTCCTTAACCTGCTGGAAGCGCAGGGGTATGCCGT
CGTACCGATGGAAAACGACGTGCTGAAGGTGGTGAAATCAAGCGCCGCGAAAGTCGAGCCGCTGCCGCTGGTCGGTGAAG
GCAGCGACAACTACGCGGGCGATGAAATGGTCACCAAAGTCGTGCCGGTACGTAATGTGTCGGTACGCGAGCTGGCACCG
ATTCTGCGCCAGATGATTGACAGCGCAGGCTCAGGCAACGTTGTTAATTACGATCCCTCCAACGTGATTATGCTCACCGG
ACGCGCCTCAGTCGTGGAGCGACTGACGGAAGTGATCCAGCGCGTGGATCACGCAGGAAACCGCACCGAAGAGGTGATCC
CGCTGGATAACGCCTCAGCCTCGGAAATTGCCCGCGTGCTGGAAAGCCTGACCAAAAACAGCGGCGAGAACCAGCCAGCA
ACGCTGAAATCTCAAATTGTCGCCGATGAACGCACCAACAGCGTGATTGTCAGTGGTGACCCCGCCACGCGAGACAAAAT
GCGCCGTCTGATCCGTCGGCTGGATTCAGAAATGGAGCGCAGAGGCAACAGCCAGGTGTTCTATCTCAAATACAGCAAAG
CCGAAGATCTGGTCGATGTGCTGAAGCAGGTCAGCGGTACGCTCACGGCGGCTAAAGAAGAGGCGGAAGGCACGGTTGGT
AGCGGGCGTGAGGTTGTCTCCATCGCCGCCAGCAAACACAGTAATGCCCTGATTGTTACTGCGCCGCAGGACATTATGCA
GTCGCTGCAAAGCGTGATTGAACAACTGGATATTCGCCGTGCTCAGGTGCATGTCGAGGCGTTAATCGTGGAAGTTGCCG
AAGGCAGCAATATCAACTTCGGCGTGCAGTGGGGGTCAAAAGATGCCGGATTAATGCAGTTTGCCAACGGTACGCAAATC
CCTATTGGTACGCTGGGGGCAGCGATTTCTGCAGCCAAGCCGCAGAAAGGTTCCACGGTGATCAGCGAAAACGGTGCTAC
CACCATAAATCCGGATACTAACGGCGATCTCTCCACGCTCGCTCAGCTTCTTTCTGGCTTTAGCGGTACGGCGGTTGGCG
TGGTGAAAGGCGACTGGATGGCGCTGGTACAGGCGGTCAAAAACGACTCCAGCTCGAACGTGCTCTCCACGCCGAGTATC
ACCACGCTGGACAACCAGGAAGCCTTCTTCATGGTGGGCCAGGACGTTCCGGTATTAACTGGATCTACCGTTGGCTCCAA
TAACAGCAATCCTTTCAATACGGTAGAGAGGAAAAAAGTCGGCATCATGCTGAAAGTCACGCCGCAGATTAACGAAGGTA
ACGCGGTACAGATGGTGATTGAGCAGGAAGTCTCGAAAGTGGAAGGGCAGACCAGCCTCGATGTCGTGTTTGGCGAGCGC
AAACTGAAAACCACCGTGCTGGCTAACGATGGTGAGCTGATCGTGCTTGGCGGTCTGATGGACGACCAGGCGGGAGAAAG
CGTGGCGAAAGTGCCGCTGCTGGGTGATATCCCGTTGATTGGTAACCTGTTTAAATCGACGGCGGATAAAAAAGAAAAAC
GTAACCTGATGGTGTTTATCCGCCCGACCATTTTGCGTGACGGTATGGCGGCAGACGGCGTGTCGCAGCGCAAATATAAC
TACATGCGCGCTGAGCAAATCTACCGCGATGAGCAAGGCTTAAGCCTGATGCCGCACACCGCGCAGCCGATATTGCCAGC
GCAAAATCAGGCCTTACCGCCGGAAGTACGCGCGTTCCTCAATGCCGGGAGAACGCGTTAA

Upstream 100 bases:

>100_bases
CAATGGATTCCATTCAACTTACGGTTTTACGCAAGGGTGCGCGCTACGACATTTCCATCGCGCTGCGCTAACCGCATTTA
ATCCAGGAGAATCATTCATC

Downstream 100 bases:

>100_bases
TGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGCA
TGGTGCGAAGCGTCGCTGGA

Product: general secretion pathway protein GspD

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVS
IRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERRGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVG
SGREVVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLMQFANGTQI
PIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGER
KLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYN
YMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNAGRTR

Sequences:

>Translated_686_residues
MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVS
IRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERRGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVG
SGREVVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLMQFANGTQI
PIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGER
KLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYN
YMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNAGRTR
>Mature_686_residues
MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVS
IRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERRGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVG
SGREVVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLMQFANGTQI
PIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGER
KLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYN
YMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNAGRTR

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=654, Percent_Identity=44.6483180428135, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789793, Length=307, Percent_Identity=24.7557003257329, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 74219; Mature: 74219

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIET
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
VGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKS
HCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHH
SAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAPILRQMIDSAGSGNVVNYDPS
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
NVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERRGNSQVFYLKYSKAEDLVDV
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
LKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRR
HHHHHCHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHH
AQVHVEALIVEVAEGSNINFGVQWGSKDAGLMQFANGTQIPIGTLGAAISAAKPQKGSTV
HHHEEEHHEEEEECCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCEE
ISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
EECCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEECCCCE
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVI
EEECCCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCEEEHHH
EQEVSKVEGQTSLDVVFGERKLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLI
HHHHHHHCCCCEEEEEECCCEEEEEEEECCCCEEEECCCCCCCCCCCEEECCCCCCCCHH
GNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHT
HHHHHHHHCHHHCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCC
AQPILPAQNQALPPEVRAFLNAGRTR
CCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIET
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
VGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKS
HCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHH
SAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAPILRQMIDSAGSGNVVNYDPS
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
NVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERRGNSQVFYLKYSKAEDLVDV
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
LKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRR
HHHHHCHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHH
AQVHVEALIVEVAEGSNINFGVQWGSKDAGLMQFANGTQIPIGTLGAAISAAKPQKGSTV
HHHEEEHHEEEEECCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCEE
ISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
EECCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEECCCCE
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVI
EEECCCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCEEEHHH
EQEVSKVEGQTSLDVVFGERKLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLI
HHHHHHHCCCCEEEEEECCCEEEEEEEECCCCEEEECCCCCCCCCCCEEECCCCCCCCHH
GNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHT
HHHHHHHHCHHHCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCC
AQPILPAQNQALPPEVRAFLNAGRTR
CCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]