Click here to switch to the map view.

The map label for this gene is sthA [H]

Identifier: 170681015

GI number: 170681015

Start: 4505213

End: 4506613

Strand: Reverse

Name: sthA [H]

Synonym: EcSMS35_4408

Alternate gene names: 170681015

Gene position: 4506613-4505213 (Counterclockwise)

Preceding gene: 170681986

Following gene: 170680148

Centisome position: 88.92

GC content: 51.82

Gene sequence:

>1401_bases
ATGCCACATTCCTACGATTACGATGCCATAGTAATAGGTTCCGGCCCCGGCGGCGAAGGCGCTGCAATGGGCCTGGTTAA
GCAAGGTGCGCGCGTCGCAGTTATCGAGCGTTATCAAAATGTTGGCGGCGGTTGCACCCACTGGGGCACCATCCCGTCGA
AAGCTCTCCGTCACGCCGTCAGCCGCATTATAGAATTTAATCAAAACCCACTTTACAGCGACCATTCGCGACTGCTCCGC
TCTTCTTTTGCCGATATCCTTAACCATGCCGATAACGTGATTAATCAACAAACGCGCATGCGTCAGGGATTTTACGAACG
TAATCACTGTGAAATATTGCAGGGAAACGCTCGCTTTGTTGACGAGCACACGCTGGCGCTGGATTGCCCGGACGGCAGCG
TTGAAACACTAACCGCTGAAAAATTTGTTATTGCCTGCGGCTCTCGTCCATATCATCCAACAGATGTTGATTTCACCCAT
CCACGCATTTACGACAGCGACTCAATTCTTAGCATGCACCACGAACCGCGCCATGTACTTATCTATGGTGCTGGAGTGAT
CGGCTGTGAATATGCGTCGATCTTCCGCGGTATGGATGTAAAAGTGGATCTGATCAACACCCGCGATCGGCTGTTGGCGT
TTCTCGATCAAGAGATGTCAGATTCTCTCTCCTATCACTTCTGGAACAGTGGCGTAGTGATTCGTCACAACGAAGAGTAC
GAGAAGATCGAAGGCTGTGACGACGGTGTGATCATGCATCTGAAGTCGGGTAAAAAACTGAAAGCTGACTGCCTGCTCTA
TGCCAACGGTCGCACCGGTAATACTGATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGA
AGGTCAACAGCATGTATCAGACCGCACAGCCGCACGTTTACGCGGTGGGCGACGTGATTGGTTATCCGAGCCTGGCGTCG
GCAGCCTATGACCAGGGGCGCATTGCCGCGCAGGCGCTGGTGAAAGGTGAAGCCAACGCACATCTGATTGAAGATATCCC
TACCGGCATTTACACCATCCCGGAAATCAGCTCTGTGGGCAAAACCGAACAGCAGCTAACCGCGATGAAAGTGCCATATG
AAGTGGGCCGCGCCCAGTTTAAACATCTGGCACGTGCACAAATCGTCGGCATGAACGTGGGCACGCTGAAAATTTTGTTC
CATCGGGAAACAAAAGAGATTCTGGGTATTCACTGCTTTGGCGAGCGCGCTGCCGAAATTATTCATATCGGTCAGGCGAT
TATGGAACAGAAAGGTGGCGGCAACACTATTGAGTACTTCGTCAACACCACCTTTAACTACCCGACGATGGCGGAAGCCT
ATCGGGTAGCTGCGCTAAATGGCTTAAACCGCCTGTTTTAA

Upstream 100 bases:

>100_bases
TCACGATATAGCCAGATAAATGACGGGGATCAATTGGCTTACCCGCGATAAAATGTTACCATTCTGTTGCTTTTATGTAT
AAGAACAGGTAAGCCCTACC

Downstream 100 bases:

>100_bases
CACTTTATCGAAATGGCCATCCATTCTTGCGCGGATGGCCTCTGCCAGCTGCTCATAGCGGCTGCGCAGTGGTGAGCCAG
GACGATAAACCAGGCCAATA

Product: soluble pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: STH; NAD(P)(+) transhydrogenase [B-specific] [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLR
SSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLAS
AAYDQGRIAAQALVKGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILF
HRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF

Sequences:

>Translated_466_residues
MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLR
SSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLAS
AAYDQGRIAAQALVKGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILF
HRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
>Mature_465_residues
PHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRS
SFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTHP
RIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEYE
KIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASA
AYDQGRIAAQALVKGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILFH
RETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF

Specific function: Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=473, Percent_Identity=28.3298097251586, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=26.4069264069264, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI22035672, Length=491, Percent_Identity=26.6802443991853, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI33519430, Length=400, Percent_Identity=25.75, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI33519428, Length=400, Percent_Identity=25.75, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI33519426, Length=400, Percent_Identity=25.75, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI148277071, Length=400, Percent_Identity=25.75, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI148277065, Length=400, Percent_Identity=25.75, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI291045266, Length=476, Percent_Identity=24.3697478991597, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI291045268, Length=350, Percent_Identity=25.4285714285714, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI65787454, Length=270, Percent_Identity=27.4074074074074, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI226437568, Length=270, Percent_Identity=27.4074074074074, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI21389617, Length=270, Percent_Identity=27.4074074074074, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=99.7854077253219, Blast_Score=970, Evalue=0.0,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=27.4089935760171, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=27.3706896551724, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=27.6102088167053, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=29.5258620689655, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=24.5283018867925, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=367, Percent_Identity=25.8855585831063, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71983419, Length=367, Percent_Identity=25.8855585831063, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=286, Percent_Identity=24.4755244755245, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=27.5933609958506, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=25.9493670886076, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=25, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=29.5116772823779, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=29.9794661190965, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=29.9794661190965, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=29.9794661190965, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=26.369168356998, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- InterPro:   IPR022962 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.1.1 [H]

Molecular weight: Translated: 51574; Mature: 51443

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAV
CCCCCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCHHHHHHHHH
SRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEE
DEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTHPRIYDSDSILSMHHEPRHVL
ECCEEEEECCCCCEEEEEECEEEEEECCCCCCCCCCCCCCCEEECCCCEEEECCCCCEEE
IYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EEECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCH
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQ
HHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEECCHHH
TAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALVKGEANAHLIEDIPTGIYTIPEISSVG
CCCCCEEEECCHHCCCCHHHHHHHCCHHHHHHHHCCCCCCEEHHHCCCCEEECCCCHHCC
KTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILFHRETKEILGIHCFGERAAEI
CCHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCHHEEEEECCHHHHHH
IHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAV
CCCCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCHHHHHHHHH
SRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEE
DEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTHPRIYDSDSILSMHHEPRHVL
ECCEEEEECCCCCEEEEEECEEEEEECCCCCCCCCCCCCCCEEECCCCEEEECCCCCEEE
IYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EEECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCH
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQ
HHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEECCHHH
TAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALVKGEANAHLIEDIPTGIYTIPEISSVG
CCCCCEEEECCHHCCCCHHHHHHHCCHHHHHHHHCCCCCCEEHHHCCCCEEECCCCHHCC
KTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILFHRETKEILGIHCFGERAAEI
CCHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCHHEEEEECCHHHHHH
IHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA