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The map label for this gene is istA [H]

Identifier: 170680910

GI number: 170680910

Start: 3231313

End: 3232485

Strand: Reverse

Name: istA [H]

Synonym: EcSMS35_3144

Alternate gene names: 170680910

Gene position: 3232485-3231313 (Counterclockwise)

Preceding gene: 170683482

Following gene: 170681926

Centisome position: 63.78

GC content: 52.86

Gene sequence:

>1173_bases
ATGCTGAGCAGAGAGGACTTTTACATGATAAAGCAAATGCGCCAGCAGGGCGCGTACATTGTCGATATTGTGACTCAGAT
TGGTTGCTCTGAACGAACGGTCAGACGCTACCTCAAATACCCTGAACCGCCAGCCAGAAGGACCCGCCACAAAATGGTTA
AGCTGAAACCGTTTATGGATTACATCGACATGCGCCTGGCAGAGAATGTCTGGAATAGCGCGGTTATCTTCGCGGAGATT
AAGGCAATGGGCTATACGGGCGGACGTTCCATGCTGCGTTACTACATCCAGCCCAACCGTAAAATGCGTCCGTCAAAAAG
AACAGTTCGCTTCGAAACTCAGCCTGGATACCAGCTCCAGCACGACTGGGGCGAAGTTGAGGTGGAAGTTGCCGGGCAAC
GGTGCAAAGTTAACTTTGCGGTTAATGCGCTGGGGTTCTCCCGCCGCTTTCATGTCTTCGCTGCCCCAAAACAGGATGCT
GAGAATACCTACGAATCACTGGTTCGCGCCTTCCGCTACTTCGGTGGTTGTGTGAAAACGGTGCTGGTTGATAACCAGAA
GGCTGCGGTGCTGAAGAATAACAACGGGAAAGTCGTGTTCAACTCCGGATTTCTGTTGCTGGCTGACCACTATAACTTCC
TGCCACGGGCATGCCGTCCACGCAGGGCCAGAACAAAAGGTAAGGTTGAGCGGATGGTGAAATACCTCAAGGAGAACTTC
TTCGTCCGGTACCGCAGGTTCGACAGCTTCACTCATGTCAATCAACAACTGGAGCAATGGATAGCCGATGTGGCTGACAA
ACGGGAGCTTCGTCAGTTCAAAGAAACGCCGGAACAGCGCTTCGCGCTGGAGCAGGAATATCTGCAGCCGTTACCGGATA
CGGACTTCGATACCAGTTACTTTGACATCCGCCATGTGTCCTGGGACAGCTATATCGAGGTTGGTGGTAATCGTTACAGC
GTTCCCGAAGCGCTGTGTGGTCAGCCGGTATCGATACGAATATCGCTGGATGACGAGTTGCGGATCTACAGTAATGAGAA
ACTGGTGGCCTCACATCGCCTCTGTTCGGCATCGTCTGGCTGGCAGACAGTGCCGGAGCATCACACCCCGCTCTGGCAGC
AGGTCAGTCAGGTGGAACATCGACTACTGAGTGCCTATGAGGAGCTGTTGTGA

Upstream 100 bases:

>100_bases
GTCAGCGCCAGTGATATAAGACGGTAATTCACCATTTGGATTGTCCGCTCCACCCAACATGTTGTTTCCTTAAGGTTCTC
ACACCAGAAAGGACATCAAC

Downstream 100 bases:

>100_bases
TGCATGAGCTGGAAGTCCTGCTGAGTCGCCTGAAAATGGAGCATCTGAGTTATCACGTTGAAAGCCTGCTGGAACAGGCA
GCTAAAAAAGAGCTGAACTA

Product: IS2 transposase orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLSREDFYMIKQMRQQGAYIVDIVTQIGCSERTVRRYLKYPEPPARRTRHKMVKLKPFMDYIDMRLAENVWNSAVIFAEI
KAMGYTGGRSMLRYYIQPNRKMRPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNALGFSRRFHVFAAPKQDA
ENTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRRARTKGKVERMVKYLKENF
FVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRFALEQEYLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYS
VPEALCGQPVSIRISLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHTPLWQQVSQVEHRLLSAYEELL

Sequences:

>Translated_390_residues
MLSREDFYMIKQMRQQGAYIVDIVTQIGCSERTVRRYLKYPEPPARRTRHKMVKLKPFMDYIDMRLAENVWNSAVIFAEI
KAMGYTGGRSMLRYYIQPNRKMRPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNALGFSRRFHVFAAPKQDA
ENTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRRARTKGKVERMVKYLKENF
FVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRFALEQEYLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYS
VPEALCGQPVSIRISLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHTPLWQQVSQVEHRLLSAYEELL
>Mature_390_residues
MLSREDFYMIKQMRQQGAYIVDIVTQIGCSERTVRRYLKYPEPPARRTRHKMVKLKPFMDYIDMRLAENVWNSAVIFAEI
KAMGYTGGRSMLRYYIQPNRKMRPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNALGFSRRFHVFAAPKQDA
ENTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRRARTKGKVERMVKYLKENF
FVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRFALEQEYLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYS
VPEALCGQPVSIRISLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHTPLWQQVSQVEHRLLSAYEELL

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: COG4584

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017894
- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 45887; Mature: 45887

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSREDFYMIKQMRQQGAYIVDIVTQIGCSERTVRRYLKYPEPPARRTRHKMVKLKPFMD
CCCCHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YIDMRLAENVWNSAVIFAEIKAMGYTGGRSMLRYYIQPNRKMRPSKRTVRFETQPGYQLQ
HHHHHHHHHHHCCEEEEEEHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEE
HDWGEVEVEVAGQRCKVNFAVNALGFSRRFHVFAAPKQDAENTYESLVRAFRYFGGCVKT
CCCCEEEEEECCCEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
VLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRRARTKGKVERMVKYLKENF
HEECCCCEEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRFALEQEYLQPLPDTDFDTSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCE
FDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRISLDDELRIYSNEKLVASHRLCSASSG
EEEEECCCCCCEEECCCEECCCHHHCCCCEEEEEEECCCEEEECCCEEHHHHHHHCCCCC
WQTVPEHHTPLWQQVSQVEHRLLSAYEELL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLSREDFYMIKQMRQQGAYIVDIVTQIGCSERTVRRYLKYPEPPARRTRHKMVKLKPFMD
CCCCHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YIDMRLAENVWNSAVIFAEIKAMGYTGGRSMLRYYIQPNRKMRPSKRTVRFETQPGYQLQ
HHHHHHHHHHHCCEEEEEEHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEE
HDWGEVEVEVAGQRCKVNFAVNALGFSRRFHVFAAPKQDAENTYESLVRAFRYFGGCVKT
CCCCEEEEEECCCEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
VLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRRARTKGKVERMVKYLKENF
HEECCCCEEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRFALEQEYLQPLPDTDFDTSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCE
FDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRISLDDELRIYSNEKLVASHRLCSASSG
EEEEECCCCCCEEECCCEECCCHHHCCCCEEEEEEECCCEEEECCCEEHHHHHHHCCCCC
WQTVPEHHTPLWQQVSQVEHRLLSAYEELL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]