Click here to switch to the map view.

The map label for this gene is ubiH [H]

Identifier: 170680909

GI number: 170680909

Start: 3130118

End: 3131296

Strand: Reverse

Name: ubiH [H]

Synonym: EcSMS35_3041

Alternate gene names: 170680909

Gene position: 3131296-3130118 (Counterclockwise)

Preceding gene: 170680916

Following gene: 170683096

Centisome position: 61.78

GC content: 60.22

Gene sequence:

>1179_bases
ATGAGCGTAATCATCGTCGGTGGCGGCATGGCGGGCGCGACGCTGGCGCTGGCTATTTCCCGGTTAAGTCACGGGGCGCT
GCCGGTACATTTGATTGAAGCGACTGCGCCAGAGTCACATGCTCATCCGGGCTTTGATGGACGAGCTATTGCGCTGGCGG
CGGGTACCTGTCAGCAACTGGCGCGCATCGGCGTTTGGCAATCTCTGGCGGATTGCGCAACCGCCATCACCAGCGTGCAT
GTCAGCGATCGTGGTCATGCCGGATTCGTCACCCTCGCCGCAGAAGATTACCAACTGGCGGCGCTGGGGCAGGTTGTCGA
ATTGCATAATGTCGGGCAACGGCTGTTTGCATTGCTGCGCAAAGCACCTGGCGTAACGCTGCATTGCCCTGATCGCGTGG
CTAACGTTGCCCGTGCTCAGAGTCACGTTGAAGTGACGCTGGAGAGTGGCGAGACGCTGACGGGCTGCGTGCTGGTAGCC
GCTGATGGCACCCATTCAGCGTTAGCCACTGTGTGCGGCGTTGACTGGCAGCAGGAGCCTTACGAACAACTGGCTGTGAT
TGCTAACGTTGCGACTTCCGTTGCGCACGAAGGCCGCGCTTTTGAACGCTTCACGAAACATGGCCCGCTGGCGATGTTGC
CGATGTCTGACGGACGCTGTTCGCTGGTCTGGTGTCATCCACTGGAACGGCGCGAAGAGGTGCTGTCGTGGAGCGACGAG
AAGTTTTGCCGTGAACTCCAGTCTGCCTTTGGCTGGCGACTGGGGCAAATTACCCACGCCGGTAAACGCAGTGCTTATCC
GCTGGCATTAACCCGCGCCGCCAGACCGATTACCCATCGCACTGTGCTGGTGGGCAATGCGGCGCAAACTCTGCACCCCA
TCGCCGGGCAAGGGTTCAACCTCGGTATGCGTGATGTGATGAGCCTTGCAGAAACCCTGACTCAGGCGCAGGAGCGCGGA
GAAGACATGGGTGATTACGGCGTATTGTGCCGTTATCAGCAGCGTCGACAGCGCGATCGCGAAGCAACCATCGGCGTCAC
GGACAGCCTTGTACATCTTTTTGCCAACCGTTGGACCCCGCTGGTTGTCGGGCGCAACATCGGGCTGATGACGATGGAAT
TATTTACCCCGGCACGCGATGTGCTGGCGCAGCGCACCCTCGGTTGGGTGGCGCGTTGA

Upstream 100 bases:

>100_bases
ACGACATTGTGATTACCGAAACCGGCAACGAAAACCTCACCGCCAGCGTGGTGAAAAAGCCGGAAGAAATCGAAGCGTTG
ATGGCTGCTGCGAGAAAGCA

Downstream 100 bases:

>100_bases
AGGCGGTTTTAAGGAATAAACAATGCAAAGTGTTGATGTAGCCATTGTTGGTGGCGGCATGGTGGGGCTGGCGGTTGCCT
GCGGCTTACAGGGGAGCGGC

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADCATAITSVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARAQSHVEVTLESGETLTGCVLVA
ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARPITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERG
EDMGDYGVLCRYQQRRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Sequences:

>Translated_392_residues
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADCATAITSVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARAQSHVEVTLESGETLTGCVLVA
ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARPITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERG
EDMGDYGVLCRYQQRRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
>Mature_391_residues
SVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADCATAITSVHV
SDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARAQSHVEVTLESGETLTGCVLVAA
DGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDEK
FCRELQSAFGWRLGQITHAGKRSAYPLALTRAARPITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGE
DMGDYGVLCRYQQRRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=29.0155440414508, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI32967307, Length=374, Percent_Identity=29.9465240641711, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=97.4489795918367, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=30.0254452926209, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1786883, Length=372, Percent_Identity=31.989247311828, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25146631, Length=425, Percent_Identity=25.4117647058824, Blast_Score=130, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=24.5033112582781, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19920760, Length=421, Percent_Identity=24.2280285035629, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42348; Mature: 42217

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQSLADCATAITSVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARAQSHVEVTLESGETLTGCVLVAADGTHSALATVCGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCHH
KFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARPITHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQRRQRDREATIGVTDSLVHLFANRWTP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCE
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
EEEECCCCEEEHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
EEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQSLADCATAITSVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARAQSHVEVTLESGETLTGCVLVAADGTHSALATVCGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCHH
KFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARPITHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQRRQRDREATIGVTDSLVHLFANRWTP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCE
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
EEEECCCCEEEHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]