BacMap

Definition	Escherichia coli SMS-3-5 chromosome, complete genome.
Accession	NC_010498
Length	5,068,389

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The map label for this gene is rfbB [H]

Identifier: 170680867

GI number: 170680867

Start: 2289443

End: 2290183

Strand: Reverse

Name: rfbB [H]

Synonym: EcSMS35_2267

Alternate gene names: 170680867

Gene position: 2290183-2289443 (Counterclockwise)

Preceding gene: 170682573

Following gene: 170679598

Centisome position: 45.19

GC content: 45.48

Gene sequence:

>741_bases
ATGGAACCAGTAATAAACTTTAGTCACGTTACGAAAGAGTATCCTCTTTACCACCATATTGGCTCAGGAATTAAGGATCT
CGTATTTCACCCTAAAAGAGCTTTTCAGCTGTTAAAAGGGCGTAAATATCTTGCGATCGAAGACATTACCTTCACCGTGG
GTAAAGGCGAGGCCGTAGCGCTGATTGGTCGTAACGGCGCAGGGAAAAGTACCTCCCTCGGGCTGGTGGCCGGCGTCATT
AAGCCTACAAAAGGCACTGTTACAACCCAGGGACGCGTTGCCTCAATGCTTGAGCTAGGCGGCGGTTTTCACCCTGAATT
AACCGGGCGAGAAAATATTTATCTCAATGCGACCCTGCTTGGGCTACGCCGCAAAGAGGTTCAGCAACGGATGGATCGGA
TAATTGAGTTCTCCGAGCTGGGTGATTTCATTGACGAACCTATTCGCGTGTACTCAAGTGGTATGCTTGCGAAGCTTGGA
TTCTCTGTCATCAGCCAGGTAGAACCCGATATACTTATTATCGATGAGGTACTTGCTGTCGGTGACATTTCGTTCCAGGC
CAAATGTATACAAACAATACGAGACTTCAAAAATAAGGGTGTAACGATATTATTCGTTAGCCACAATATGAGTGATGTTG
AAAAAATCTGTGACAGAGTTGTTTGGATCGAAAACCATAAACTCCGCGAAATTGGCTCAGCAGAGAGAATCATTGAACTT
TACAAGCAAGCAATGGCTTAA

Upstream 100 bases:

>100_bases
ATGAATATATTTCAATCCTTTACATTACAGGTTTAGTCCTGACAATTGTGGGCCTGAGTATTTTTAATAAATTAAAATAT
CGATTTGCAGAGATTTTGTA

Downstream 100 bases:

>100_bases
TAATTGGTAATAAGATGAATAGTATCAAAATTTATACATGTCACCACAAACCCAGTGCTTTTCTAAATGCCTCTATTATT
AAACCACTGCATGTCGGGAA

Product: O-antigen export system ATP-binding protein RfbB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MEPVINFSHVTKEYPLYHHIGSGIKDLVFHPKRAFQLLKGRKYLAIEDITFTVGKGEAVALIGRNGAGKSTSLGLVAGVI
KPTKGTVTTQGRVASMLELGGGFHPELTGRENIYLNATLLGLRRKEVQQRMDRIIEFSELGDFIDEPIRVYSSGMLAKLG
FSVISQVEPDILIIDEVLAVGDISFQAKCIQTIRDFKNKGVTILFVSHNMSDVEKICDRVVWIENHKLREIGSAERIIEL
YKQAMA

Sequences:

>Translated_246_residues
MEPVINFSHVTKEYPLYHHIGSGIKDLVFHPKRAFQLLKGRKYLAIEDITFTVGKGEAVALIGRNGAGKSTSLGLVAGVI
KPTKGTVTTQGRVASMLELGGGFHPELTGRENIYLNATLLGLRRKEVQQRMDRIIEFSELGDFIDEPIRVYSSGMLAKLG
FSVISQVEPDILIIDEVLAVGDISFQAKCIQTIRDFKNKGVTILFVSHNMSDVEKICDRVVWIENHKLREIGSAERIIEL
YKQAMA
>Mature_246_residues
MEPVINFSHVTKEYPLYHHIGSGIKDLVFHPKRAFQLLKGRKYLAIEDITFTVGKGEAVALIGRNGAGKSTSLGLVAGVI
KPTKGTVTTQGRVASMLELGGGFHPELTGRENIYLNATLLGLRRKEVQQRMDRIIEFSELGDFIDEPIRVYSSGMLAKLG
FSVISQVEPDILIIDEVLAVGDISFQAKCIQTIRDFKNKGVTILFVSHNMSDVEKICDRVVWIENHKLREIGSAERIIEL
YKQAMA

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbA [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=250, Percent_Identity=31.2, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI6005701, Length=214, Percent_Identity=32.2429906542056, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI27477115, Length=250, Percent_Identity=30.8, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI27436953, Length=242, Percent_Identity=30.5785123966942, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI31657092, Length=223, Percent_Identity=28.2511210762332, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI27262624, Length=222, Percent_Identity=27.4774774774775, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI27262626, Length=222, Percent_Identity=27.4774774774775, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI27881501, Length=217, Percent_Identity=24.8847926267281, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI30795238, Length=217, Percent_Identity=24.8847926267281, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI150417984, Length=213, Percent_Identity=25.8215962441315, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI45446740, Length=209, Percent_Identity=27.7511961722488, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI47078218, Length=209, Percent_Identity=27.7511961722488, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI105990541, Length=213, Percent_Identity=25.3521126760563, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI116734710, Length=201, Percent_Identity=26.3681592039801, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI21536376, Length=235, Percent_Identity=24.2553191489362, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI87081709, Length=212, Percent_Identity=26.4150943396226, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1789891, Length=205, Percent_Identity=27.3170731707317, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786319, Length=205, Percent_Identity=28.780487804878, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI48995001, Length=197, Percent_Identity=25.8883248730964, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790467, Length=206, Percent_Identity=24.2718446601942, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787089, Length=219, Percent_Identity=26.4840182648402, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081791, Length=206, Percent_Identity=23.7864077669903, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787758, Length=216, Percent_Identity=25.462962962963, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994943, Length=211, Percent_Identity=25.5924170616114, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081782, Length=197, Percent_Identity=26.9035532994924, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789032, Length=237, Percent_Identity=23.2067510548523, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786398, Length=222, Percent_Identity=25.6756756756757, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788761, Length=207, Percent_Identity=23.6714975845411, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786253, Length=202, Percent_Identity=27.2277227722772, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789962, Length=286, Percent_Identity=22.027972027972, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789593, Length=217, Percent_Identity=26.7281105990783, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788210, Length=207, Percent_Identity=28.0193236714976, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788897, Length=208, Percent_Identity=26.4423076923077, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790525, Length=199, Percent_Identity=26.6331658291457, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787370, Length=205, Percent_Identity=21.9512195121951, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787712, Length=200, Percent_Identity=22, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787029, Length=209, Percent_Identity=22.488038277512, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87082268, Length=199, Percent_Identity=27.1356783919598, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=212, Percent_Identity=25, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=208, Percent_Identity=23.5576923076923, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17510237, Length=207, Percent_Identity=21.7391304347826, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221500365, Length=197, Percent_Identity=27.4111675126904, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI116007184, Length=197, Percent_Identity=27.4111675126904, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221512771, Length=250, Percent_Identity=26, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24666092, Length=209, Percent_Identity=25.3588516746411, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116007328, Length=207, Percent_Identity=25.1207729468599, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574150, Length=207, Percent_Identity=25.1207729468599, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24643674, Length=208, Percent_Identity=23.5576923076923, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28573571, Length=191, Percent_Identity=24.0837696335079, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573573, Length=191, Percent_Identity=24.0837696335079, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.30 [C]

Molecular weight: Translated: 27369; Mature: 27369

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPVINFSHVTKEYPLYHHIGSGIKDLVFHPKRAFQLLKGRKYLAIEDITFTVGKGEAVA
CCCCCCHHHHHHCCCHHHHHCCCHHHHHCCCHHHHHHHCCCCEEEEEEEEEEECCCCEEE
LIGRNGAGKSTSLGLVAGVIKPTKGTVTTQGRVASMLELGGGFHPELTGRENIYLNATLL
EEECCCCCCCCCHHHHHHEECCCCCCEEECHHHHHHHHHCCCCCCCCCCCCEEEEEHHHH
GLRRKEVQQRMDRIIEFSELGDFIDEPIRVYSSGMLAKLGFSVISQVEPDILIIDEVLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEHHHHH
GDISFQAKCIQTIRDFKNKGVTILFVSHNMSDVEKICDRVVWIENHKLREIGSAERIIEL
CCCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHEEECCCCHHHHCCCHHHHHHH
YKQAMA
HHHHCC
>Mature Secondary Structure
MEPVINFSHVTKEYPLYHHIGSGIKDLVFHPKRAFQLLKGRKYLAIEDITFTVGKGEAVA
CCCCCCHHHHHHCCCHHHHHCCCHHHHHCCCHHHHHHHCCCCEEEEEEEEEEECCCCEEE
LIGRNGAGKSTSLGLVAGVIKPTKGTVTTQGRVASMLELGGGFHPELTGRENIYLNATLL
EEECCCCCCCCCHHHHHHEECCCCCCEEECHHHHHHHHHCCCCCCCCCCCCEEEEEHHHH
GLRRKEVQQRMDRIIEFSELGDFIDEPIRVYSSGMLAKLGFSVISQVEPDILIIDEVLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEHHHHH
GDISFQAKCIQTIRDFKNKGVTILFVSHNMSDVEKICDRVVWIENHKLREIGSAERIIEL
CCCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHEEECCCCHHHHCCCHHHHHHH
YKQAMA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7533882 [H]