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The map label for this gene is glcF [H]

Identifier: 170680842

GI number: 170680842

Start: 3332347

End: 3333570

Strand: Reverse

Name: glcF [H]

Synonym: EcSMS35_3255

Alternate gene names: 170680842

Gene position: 3333570-3332347 (Counterclockwise)

Preceding gene: 170682017

Following gene: 170681117

Centisome position: 65.77

GC content: 55.96

Gene sequence:

>1224_bases
ATGCAAACCCAATTAACCGAAGAGATGCGGCAGAACGCGCGGGCGCTGGAAGCCGACAGCATCCTGCGCGCCTGTGTTCA
CTGCGGATTTTGTACCGCAACCTGCCCAACCTATCAGCTCCTGGGCGATGAACTGGACGGGCCGCGCGGGCGCATCTATC
TGATTAAACAGGTGCTGGAAGGCAACGAAGTCACGCTTAAAACACAGGAGCATCTCGATCGCTGTCTCACTTGCCGCAAT
TGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTTGCTGGATATCGGGCGTGATATTGTCGAGCAGAAAGTGAA
ACGCCCATTGCCGGAGCGAATGCTGCGCGAAGGATTGCGCCAGGTAGTGCCGCGTCCGGCGGTCTTCCGTGCGCTGACGC
AGGTAGGACTGGTGCTGCGACCGTTTCTGCCGGAACAGGTCAGAGCAAAACTGCCCGCCGAAACGGTGAAAGCTAAACCG
CGACCACCGCTGCGCCATAAGCGTCGGGTTTTAATGCTGGAAGGCTGCGCCCAGCCTACGCTTTCGCCCAACACCAACGC
GGCAACTGCGCGAGTGCTGGATCGTCTGGGGATCAGCGTCATGTCAGCTAACGAAGCAGGCTGTTGTGGCGCGGTGGACT
ATCATCTTAATGCGCAGGAGAAAGGGCTGGCACGGGCGCGCAATAATATTGATGCCTGGTGGCCCGCGATTGAAGCAGGT
GCCGAGGCAATTTTGCAAACCGCCAGCGGTTGCGGCGCGTTTGTCAAAGAGTACGGGCAGATGCTGAAAAACGATGCGTT
ATATGCCGATAAAGCGCGTCAAGTCAGTGAACTGGCGGTCGATTTAGTCGAGCTTCTGCGCGAGGAACCGCTGGAAAAAC
TGGCAATTCGCGGCGATAAAAAACTGGCCTTCCACTGCCCGTGTACCCTACAACATGCGCAAAAGCTGAACGGCGAAGTG
GAAAAAGTGTTGCTTCGTCTTGGATTTACCTTAACGGACGTTCCCGACAGCCATCTGTGCTGCGGTTCAGCGGGAACATA
TGCGTTAACGCATCCCGATCTGGCACGCCAGCTGCGGGAAAACAAAATGAATGCGCTGGAAAGCGGCAAACCGGAAATGA
TCGTCACCGCCAACATTGGTTGCCAGACCCATCTGGCGAGCGCCGGTCGTACCTCTGTGCGTCACTGGATTGAAATTGTA
GAACAAGCCCTTGAAAAGGAATAA

Upstream 100 bases:

>100_bases
GCTCCTTTATTCCGCTATCACCAGCAGCTTAAACAGCAGCTCGACCCTTGCGGCGTGTTTAATCCCGGTCGCATGTACGC
GGAGCTTTAAGGAGCAGGCT

Downstream 100 bases:

>100_bases
CAAAATGAAAACTAAAGTCATTCTTAGCCAGCAAATGGCGAGTGCAATTATTGCCGCAGGTCAGGAAGAGGCGCAGAAAA
ATAACTGGTCTGTTTCCATT

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRN
CETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEV
EKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRENKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIV
EQALEKE

Sequences:

>Translated_407_residues
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRN
CETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEV
EKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRENKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIV
EQALEKE
>Mature_407_residues
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRN
CETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEV
EKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRENKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIV
EQALEKE

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=407, Percent_Identity=99.2628992628993, Blast_Score=829, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45133; Mature: 45133

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERMLREGLR
CCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
QVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKPRPPLRHKRRVLMLEGCAQPT
HHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCCCHHHHCCEEEEECCCCCC
LSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
CCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHH
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDK
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
KLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRE
CEEEECCCHHHHHHHHCCHHHHHHHHHCCCEECCCCCCEEECCCCCEEEECHHHHHHHHH
NKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIVEQALEKE
HHHHHHHCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERMLREGLR
CCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
QVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKPRPPLRHKRRVLMLEGCAQPT
HHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCCCHHHHCCEEEEECCCCCC
LSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
CCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHH
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDK
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
KLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRE
CEEEECCCHHHHHHHHCCHHHHHHHHHCCCEECCCCCCEEECCCCCEEEECHHHHHHHHH
NKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIVEQALEKE
HHHHHHHCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]