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The map label for this gene is phnJ [H]

Identifier: 170680761

GI number: 170680761

Start: 4664258

End: 4665103

Strand: Reverse

Name: phnJ [H]

Synonym: EcSMS35_4564

Alternate gene names: 170680761

Gene position: 4665103-4664258 (Counterclockwise)

Preceding gene: 170680400

Following gene: 170681420

Centisome position: 92.04

GC content: 57.92

Gene sequence:

>846_bases
ATGGCTAATCTGAGCGGCTACAACTTTGCCTACCTCGACGAGCAGACCAAACGCATGATCCGCCGCGCCATCTTAAAAGC
GGTGGCGATCCCCGGTTATCAGGTGCCGTTTGGCGGGCGCGAGATGCCGATGCCGTACGGCTGGGGAACTGGTGGTATCC
AGCTTACCGCCAGCGTGATTGGCGAAAGCGACGTGTTGAAGGTGATTGACCAGGGTGCGGATGACACCACCAACGCGGTG
TCGATCCGTAACTTCTTTAAGCGCGTGACCGGGGTAAACACCACTGAACGTACGGACGATGCGACGCTTATCCAGACGCG
CCACCGCATCCCCGAAACGCCGCTGACCGAAGATCAGATCATTATTTTCCAGGTGCCGATCCCCGAACCGCTGCGCTTTA
TCGAGCCGCGCGAAACGGAGACCCGCACCATGCACGCGCTGGAGGAGTACGGCGTGATGCAGGTGAAACTGTATGAAGAT
ATCGCCCGCTTCGGTCATATCGCCACCACCTACGCCTATCCGGTGAAGGTAAACGGGCGCTACGTAATGGACCCGTCGCC
GATCCCGAAATTCGATAACCCAAAAATGGACATGATGCCCGCCCTGCAACTGTTCGGCGCGGGGCGCGAGAAGCGCATCT
ATGCGGTGCCGCCGTTTACCCGCGTGGAAAGCCTCGATTTCGACGATCACCCGTTCACCGTTCAGCAGTGGAATGAGCCA
TGCGCCATCTGCGGATCGACCCACAGCTATCTCGACGAAGTGGTACTGGATGACGCCGGAAACCGCATGTTTGTCTGCTC
CGATACCAATTATTGCCGCCAACAGAGCGAGGCAAAAAACCAATGA

Upstream 100 bases:

>100_bases
GTCGAAGCCGCAGGCTTTGTCTCGCACCTTAAACTCCCGCACTACGTCGATTTCCAGGCCGAACTGGAGCTACTCAAACG
TCTGCAACAGGAGCGGAACC

Downstream 100 bases:

>100_bases
ATCAACCGTTACTTTCGGTCAATAACCTGACCCACCTTTACGCGCCGGGCAAAGGCTTTAGCGATGTCTCTTTTGATTTA
TGGCCGGGGGAAGTGCTGGG

Product: phosphonate metabolism protein PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAV
SIRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWNEP
CAICGSTHSYLDEVVLDDAGNRMFVCSDTNYCRQQSEAKNQ

Sequences:

>Translated_281_residues
MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAV
SIRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWNEP
CAICGSTHSYLDEVVLDDAGNRMFVCSDTNYCRQQSEAKNQ
>Mature_280_residues
ANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVS
IRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYEDI
ARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWNEPC
AICGSTHSYLDEVVLDDAGNRMFVCSDTNYCRQQSEAKNQ

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=281, Percent_Identity=98.932384341637, Blast_Score=583, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 31858; Mature: 31726

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVI
CCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEEHH
GESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQI
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCE
IIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYEDIARFGHIATTYAYPVKVNGR
EEEECCCCCCHHHCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHCCCHHEEEEEEEECCE
YVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWNEP
EEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCCCCCCCHHCCCCC
CAICGSTHSYLDEVVLDDAGNRMFVCSDTNYCRQQSEAKNQ
CEECCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure 
ANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVI
CCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEEHH
GESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQI
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCE
IIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYEDIARFGHIATTYAYPVKVNGR
EEEECCCCCCHHHCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHCCCHHEEEEEEEECCE
YVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWNEP
EEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCCCCCCCHHCCCCC
CAICGSTHSYLDEVVLDDAGNRMFVCSDTNYCRQQSEAKNQ
CEECCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]