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The map label for this gene is yqiG [H]

Identifier: 170680739

GI number: 170680739

Start: 330089

End: 331231

Strand: Reverse

Name: yqiG [H]

Synonym: EcSMS35_0325

Alternate gene names: 170680739

Gene position: 331231-330089 (Counterclockwise)

Preceding gene: 170680060

Following gene: 170681094

Centisome position: 6.54

GC content: 44.01

Gene sequence:

>1143_bases
ATGACAAATAAACACTCATCATTGTTTTCTCCTTTTATGCTGACAGAAAAAATAAAGCTACGTAATCGTATCGTTATGGC
TCCTATGACAACCTGGTCAGCTAATCCTGATGGAACCATCTCTGAGCAAGAACTCGAATTTTATAAACGTCGCTCACAAA
ATGTTGGTTTAGTAATAACAGGATGCACCTATGTCACGCCAAGCGGCATTGGGTTTACTCATGAATTTGCCGCGTATGAC
GACCGTTTCATTAACAGCCTTGAAAAGTTAGCAGCTGCAGCCAAAAGTGGCGGTGCTCCGGCTATTTTGCAGATTTTCCA
CGCCGGTAATAAAGCCATCCCGGAGCTTGTGCCAAATAACGATGTTATTAGTGCCAGCGCATCAAGTGTAAAATCTGGTG
ACTTTATGAAGAGAGTGGTACAAAGCAGGGAAATGACTGAAAACGAAATTCAGGAAACCATTAGAGCATTCGGAGATGTC
ACAAAAAGGGCGATTAAAGCAGGTTTTGATGGCGTTGAACTTCATGGTGCGCACGGATTCCTTCTGCAGAATTTCTTCTC
CCCTCTATTTAACCAGCGTAATGACCGATGGGGAGGAGATCTTGAAGGACGTATGCGCTTCCCGCTGGCCGTATTACAGG
AAGTAAAAAATGTAGTCTATGAATATGCGACAAAGCCCTTTGCTATCGGTTACAGAATATCTCCTGAAGAATCTGTAACA
GGGGGGCTGCGGATAGAGGATACTTATAAACTCCTTGATCGGCTCATTTCATCCGGTATTTCGTATATACATACCTCGCT
GGTCAGTATTAACGATAGCTACCCTGTTGAGTCGCCAAATGGCCCCCGAACAATTGAATTGATTTTAAATCATATTGCAG
GCAGAGTGCCGGTAATCGCCGCAGGTAAAATACGCACACCGTCTCAGGCTCAAGAAGCAATTTCCGCTGGCCTGCCACTT
GTTGCGATCGGTAAAGGGCTGGTGATAAATCCAGAATGGGTGACTTTAGCTGAATCAGGTAGAAGTCATGAAATTCAAAC
AGCCCTTAATCCACAACGCGTTCCTGAATTAACCATTCCGGATAAACTATGGGATCAAATTCAGGCATCGAAAGGAACTG
GCTGGTTTCCGTTAATAGATTAA

Upstream 100 bases:

>100_bases
GGGATTATAAATAAAGCCAGAAATCTTGGTTCCAAAGCTGGCAACAACTAACCAGTTAAAACATACGCATTAAAATCAGA
TAAACATTTGAGGTATTGTT

Downstream 100 bases:

>100_bases
ATATCGATTACTCTCCCGAGAGTAAAACTATCAAATCAATGAGTAACCGATACAGCCTCACAAATTCTTTCAGATAATTA
ACCCGTCACTGCGGCACGTT

Product: oxidoreductase, FAD/FMN-binding

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTNKHSSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYD
DRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESVT
GGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPL
VAIGKGLVINPEWVTLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLID

Sequences:

>Translated_380_residues
MTNKHSSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYD
DRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESVT
GGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPL
VAIGKGLVINPEWVTLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLID
>Mature_379_residues
TNKHSSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDD
RFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVT
KRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESVTG
GLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLV
AIGKGLVINPEWVTLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLID

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=352, Percent_Identity=28.6931818181818, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1787939, Length=362, Percent_Identity=28.7292817679558, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=381, Percent_Identity=25.1968503937008, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=378, Percent_Identity=25.6613756613757, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=260, Percent_Identity=28.8461538461538, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=277, Percent_Identity=26.7148014440433, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=272, Percent_Identity=27.2058823529412, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=221, Percent_Identity=31.2217194570136, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=185, Percent_Identity=30.2702702702703, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=356, Percent_Identity=26.123595505618, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325086, Length=361, Percent_Identity=24.9307479224377, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 41824; Mature: 41693

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKHSSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVIT
CCCCCCHHHCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNN
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
DVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGVELHGAHGF
CEEECCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHH
LLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESVT
HHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
GGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
AGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWVTLAESGRSHEIQTALNPQRVPELTIP
ECCCCCCHHHHHHHHCCCCEEEECCCEEECCCEEEEECCCCCCEEHHCCCHHHCCCCCCC
DKLWDQIQASKGTGWFPLID
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TNKHSSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVIT
CCCCCHHHCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNN
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
DVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGVELHGAHGF
CEEECCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHH
LLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESVT
HHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
GGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
AGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWVTLAESGRSHEIQTALNPQRVPELTIP
ECCCCCCHHHHHHHHCCCCEEEECCCEEECCCEEEEECCCCCCEEHHCCCHHHCCCCCCC
DKLWDQIQASKGTGWFPLID
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]