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The map label for this gene is ykgA [H]

Identifier: 170680656

GI number: 170680656

Start: 341410

End: 342267

Strand: Reverse

Name: ykgA [H]

Synonym: EcSMS35_0332

Alternate gene names: 170680656

Gene position: 342267-341410 (Counterclockwise)

Preceding gene: 170681931

Following gene: 170682093

Centisome position: 6.75

GC content: 42.77

Gene sequence:

>858_bases
ATGATCAGGCAGAAGATTCTACAGCAGCTCCTGGAGTGGATTGAGTGCAATCTTGAGCACCCTATTTCAATCGAAGATAT
CGCACAGAAATCTGGCTACAGCAGACGCAACATCCAGCTTCTGTTCCGAAATTTCATGCATGTGCCTTTGGGAGAATACA
TTCGCAAACGAAGGCTTTGTCGTGCCGCCATTCTTGTCCGGCTCTCCGCGAAATCTATGCTTGATATTGCACTCTCTTTG
CATTTTGATTCACAGCAATCATTCAGCCGAGAATTTAAAAAGTTATTCGGCTGCTCTCCCCGTGAATACCGCCACCGTGA
TTATTGGGATCTCGCAAATATCTTCCCTTCTTTTTTAATACGTCAACAGCAAAAGACGGAGTGTAGATTAGTCAACTTTC
CTGAGACACCTATTTTTGGCAATTCATTTAAATATTATATTGAAGTGTCGAATAAATCACCGGATGAAGAAGTCAAACTG
CGACGTCATCATTTAGTCAGATGTATGAAGAATTTTAAGACGGATATCTATTTCGTCTCCACGTTCGAACCGTCAACAAA
ATCGGTCGATTTGCTCACAGTTGAAACTTTTGCCGGTACGGTATGCAAAGAAACCGATTCAGAGATGTCGAAAGAGTGGA
CAATTAACCGAGGACTGTATGCCTCTTTCCGCTATGAAGGTGAGTGGGAGCTCTACCCTGAATGGACGCGAAACCTCTAT
CTGATGGAATTACCCGCCAGGAGGTTAGCCAGAATGAACGGCAGTGATATTGAGCGATTTTATTACAATGAAGATTTCGT
AGAAAAGGATAGCAATGATGTTGTTTGCGAAATATTTATCCCTGTTCGTCCGGTTTAG

Upstream 100 bases:

>100_bases
CTATCAGTGCTTTTGCCTTGGGACTTGCTGTTTTTGATACACTTAGGGCCCAGGACATAACGAAAATGTGCGATATGACA
AAGAATTAACGGAGTATGTG

Downstream 100 bases:

>100_bases
TTATCGCTATATCTAAATGAATTTATAACGAGACTACTGTCAGGCAATTCAATAAATAATGGCGTTGAAGATGTCAACGC
CATTATTGTTTATTAGAATG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSL
HFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYYIEVSNKSPDEEVKL
RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKETDSEMSKEWTINRGLYASFRYEGEWELYPEWTRNLY
LMELPARRLARMNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV

Sequences:

>Translated_285_residues
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSL
HFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYYIEVSNKSPDEEVKL
RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKETDSEMSKEWTINRGLYASFRYEGEWELYPEWTRNLY
LMELPARRLARMNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV
>Mature_285_residues
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSL
HFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYYIEVSNKSPDEEVKL
RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKETDSEMSKEWTINRGLYASFRYEGEWELYPEWTRNLY
LMELPARRLARMNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=291, Percent_Identity=31.2714776632302, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790497, Length=106, Percent_Identity=45.2830188679245, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87081928, Length=98, Percent_Identity=37.7551020408163, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 34113; Mature: 34113

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLC
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLI
HHHHHHHHHHHHHEEEHEEEECCCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHH
RQQQKTECRLVNFPETPIFGNSFKYYIEVSNKSPDEEVKLRRHHLVRCMKNFKTDIYFVS
HHCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
TFEPSTKSVDLLTVETFAGTVCKETDSEMSKEWTINRGLYASFRYEGEWELYPEWTRNLY
ECCCCCCCEEEEEEHHHHCHHHHCCHHHHHHHEEECCCEEEEEEECCCEEECCCCCCCEE
LMELPARRLARMNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV
EEECCHHHHHHCCCCCHHHHCCCCHHHCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLC
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLI
HHHHHHHHHHHHHEEEHEEEECCCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHH
RQQQKTECRLVNFPETPIFGNSFKYYIEVSNKSPDEEVKLRRHHLVRCMKNFKTDIYFVS
HHCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
TFEPSTKSVDLLTVETFAGTVCKETDSEMSKEWTINRGLYASFRYEGEWELYPEWTRNLY
ECCCCCCCEEEEEEHHHHCHHHHCCHHHHHHHEEECCCEEEEEEECCCEEECCCCCCCEE
LMELPARRLARMNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV
EEECCHHHHHHCCCCCHHHHCCCCHHHCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]