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The map label for this gene is gidA [H]

Identifier: 170680590

GI number: 170680590

Start: 4203543

End: 4205432

Strand: Reverse

Name: gidA [H]

Synonym: EcSMS35_4109

Alternate gene names: 170680590

Gene position: 4205432-4203543 (Counterclockwise)

Preceding gene: 170683215

Following gene: 170682169

Centisome position: 82.97

GC content: 54.71

Gene sequence:

>1890_bases
ATGTTTTATCCGGATCCTTTTGACGTCATCATCATTGGCGGGGGTCATGCAGGCACCGAGGCCGCGATGGCCGCGGCGCG
TATGGGTCAACAGACTCTGCTTTTGACACACAATATCGACACTCTGGGGCAGATGAGCTGCAACCCGGCGATCGGCGGTA
TTGGGAAGGGACATCTGGTAAAAGAAGTGGATGCACTCGGCGGTCTGATGGCGAAAGCGATCGATCTGGCGGGTATCCAG
TTTAGGATACTAAACGCAAGTAAGGGACCGGCAGTTCGCGCTACCCGAGCTCAGGCGGATCGTGTGCTCTACCGGCAGGC
GGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGGCAGTTGAAGATCTTATTGTCGAAAACGATC
GCGTGGTCGGAGCCGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGCTGTCGTGCTCACCGTTGGGACGTTCCTCGAC
GGTAAAATTCATATCGGTCTGGATAACTACAGCGGTGGCCGTGCTGGTGATCCGCCGTCCATTCCGCTTTCTCGCCGTTT
GCGTGAACTGCCGCTGCGCGTTGGTCGTCTGAAAACCGGGACACCACCGCGTATTGATGCTCGAACCATCGACTTTAGCG
TACTGGCGCAACAGCATGGCGATAACCCAATGCCGGTATTCTCGTTTATGGGCAATGCGTCCCAGCATCCCCAGCAGGTG
CCGTGTTATATCACTCATACCAACGAGAAAACCCATGATGTGATCCGCAGTAACCTCGATCGTAGCCCAATGTACGCAGG
GGTGATCGAAGGTGTCGGCCCACGCTACTGCCCGTCGATCGAAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATC
AGATCTTCCTTGAACCGGAAGGGCTGACCTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTG
CAGATGCAAATCGTCCGCTCCATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTT
CTTCGATCCACGCGACCTGAAACCGACGCTGGAGAGCAAGTTTATCCAGGGGCTGTTCTTTGCTGGTCAGATTAACGGCA
CTACCGGTTACGAAGAAGCCGCTGCGCAAGGTTTGCTGGCTGGTCTTAACGCTGCCCGTCTGTCTGCAGACAAAGAAGGT
TGGGCTCCGGCGCGTTCTCAGGCGTATCTCGGCGTACTGGTTGATGACCTGTGCACTTTAGGAACCAAAGAACCGTATCG
TATGTTTACCTCGCGCGCAGAATATCGTCTGATGCTGCGCGAAGATAATGCGGATCTGCGTTTGACTGAAATCGGTCGTG
AACTGGGCCTGGTGGATGACGAACGTTGGGCGCGCTTTAACGAGAAACTTGAGAATATCGAGCGTGAGCGTCAGCGTCTG
AAATCGACCTGGGTAACCCCGTCGGCGGAAGCTGCAGCCGAAGTGAATGCTCACCTGACTGCGCCACTTTCCCGTGAAGC
CAGTGGTGAAGATCTGCTGCGTCGTCCGGAAATGACTTATGAAAAATTAACCACGCTGACGCCGTTTGCCCCTGCGTTGA
CAGACGAACAGGCGGCGGAACAGGTTGAGATTCAGGTTAAATACGAAGGTTATATCGCGCGCCAGCAAGATGAGATCGAA
AAGCAGCTGCGTAACGAGAACACCCTGCTACCAGCGACGCTGGATTACCGCCAGGTATCCGGTCTTTCTAACGAAGTGAT
CGCCAAACTTAACGATCACAAACCGGCCTCTATCGGTCAGGCTTCGCGTATTTCTGGCGTCACGCCTGCGGCCATCTCCA
TTCTGCTGGTGTGGCTGAAAAAACAGGGTATGCTGCGTCGTAGCGCATAA

Upstream 100 bases:

>100_bases
TACCCAGGATCCCAGGTCTTTCTCAAGCCGACAAAGTTGAGTAGAATCCACGGCCCGGGCTTCAATCCATTTTCATACCG
CGTTATGCGAGGCAATCACC

Downstream 100 bases:

>100_bases
CGCATTAAAAATGCCTGGTAAGCACCCGCTTACCAGGCAACGCATCAAGAACAGGTAATCACCGTGCTCAACAAACTCTC
CTTACTGCTGAAAGACGCAG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDLAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA

Sequences:

>Translated_629_residues
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDLAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA
>Mature_629_residues
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDLAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=631, Percent_Identity=47.3851030110935, Blast_Score=527, Evalue=1e-149,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=45.5792682926829, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI183227703, Length=671, Percent_Identity=44.5603576751118, Blast_Score=508, Evalue=1e-144,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=99.8410174880763, Blast_Score=1295, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=637, Percent_Identity=41.1302982731554, Blast_Score=479, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=46.2035541195477, Blast_Score=526, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24658174, Length=642, Percent_Identity=46.8847352024922, Blast_Score=534, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69507; Mature: 69507

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAKAIDLAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPS
HHHHHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEEEEECCEEEEEECCCCCCCCCCCCC
IPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
CCHHHHHHHCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPE
CEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEGWAPARSQAYLGVLVDDLCTL
HHHHEEEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAE
HHHCCCCCHHHHHHCCCEEECCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCHHCCC
QVEIQVKYEGYIARQQDEIEKQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCEEEEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGVTPAAISILLVWLKKQGMLRRSA
HHHCCCCCHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAKAIDLAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPS
HHHHHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEEEEECCEEEEEECCCCCCCCCCCCC
IPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
CCHHHHHHHCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPE
CEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEGWAPARSQAYLGVLVDDLCTL
HHHHEEEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAE
HHHCCCCCHHHHHHCCCEEECCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCHHCCC
QVEIQVKYEGYIARQQDEIEKQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCEEEEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGVTPAAISILLVWLKKQGMLRRSA
HHHCCCCCHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA