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The map label for this gene is phnI [H]

Identifier: 170680400

GI number: 170680400

Start: 4665096

End: 4666160

Strand: Reverse

Name: phnI [H]

Synonym: EcSMS35_4565

Alternate gene names: 170680400

Gene position: 4666160-4665096 (Counterclockwise)

Preceding gene: 170683832

Following gene: 170680761

Centisome position: 92.06

GC content: 61.5

Gene sequence:

>1065_bases
ATGTACGTTGCCGTGAAAGGGGGCGAGAAGGCGATCGACGCCGCCCACGCCCTGCAAGAGAGCCGACGCCGGGGCAATAC
CGATTTGCCTGAACTGAGCGTCGCCCAGATTGAACAGCAGCTTAATCTTGCAGTAGATCGCGTGATGACCGAAGGCGGCA
TTGCCGACCGCGAACTGGCGGCGCTGGCGCTGAAACAGGCCAGCGGCGATAACGTCGAAGCGATTTTCCTGCTACGCGCC
TACCGCACCACGCTGGCGAAGCTTGCGGTGAGCGAGCCGCTCGACACCACGGAGATGCGTCTCGAACGGCGTATTTCAGC
GGTTTATAAAGATATTCCCGGCGGCCAGCTGCTTGGCCCAACCTACGACTACACCCATCGCCTGCTCGATTTCACCCTGC
TGGCAAACGGCGAAGCGCCGACGCTGACCACAGCCGACAGCGAACAACAGCCGTCGCCGCACGTTTTCAGCCTGCTGGCG
CGTCAGGGGCTGGCGAAGTTTGAAGAAGATAGCGGCGCACAGCCGGACGACATCACCCGCACGCCGCCGGTTTACCCCTG
CTCACGCTCCTCCCGCCTGCAACAATTAATGCGCGGCGACGAAGGCTATTTGCTGGCGCTGGCCTACTCCACCCAGCGTG
GTTACGGACGCAATCATCCGTTCGCAGGCGAAATCCGCAGCGGCTATATCGACGTGTCGATTGTGCCGGAAGAGCTGGGA
TTTGCGGTGAACGTCGGCGAACTGCTGATGACCGAATGTGAAATGGTCAACGGTTTTATCGACCCGCCGGATGAGCCGCC
GCACTTCACGCGCGGCTACGGGCTGGTGTTCGGCATGAGCGAGCGCAAAGCGATGGCGATGGCGCTGGTTGACCGCGCTC
TACAAGCCCCGGAGTACGGCGAGCACGCGACAGGCCCGGCGCAGGATGAAGAGTTCGTGCTGGCGCATGCCGACAACGTC
GAAGCCGCAGGCTTTGTCTCGCACCTTAAACTCCCGCACTACGTCGATTTCCAGGCCGAACTGGAGCTACTCAAACGTCT
GCAACAGGAGCGGAACCATGGCTAA

Upstream 100 bases:

>100_bases
CCGAGCGCCCGCACCCGTTCCCGCTCGGCATCGACCTGATCCTGACCTGCGGCGAGCGCCTGCTGGCTATCCCGCGAACC
ACTCATGTGGAGGTGTGCTG

Downstream 100 bases:

>100_bases
TCTGAGCGGCTACAACTTTGCCTACCTCGACGAGCAGACCAAACGCATGATCCGCCGCGCCATCTTAAAAGCGGTGGCGA
TCCCCGGTTATCAGGTGCCG

Product: phosphonate metabolism protein PhnI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELAALALKQASGDNVEAIFLLRA
YRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNV
EAAGFVSHLKLPHYVDFQAELELLKRLQQERNHG

Sequences:

>Translated_354_residues
MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELAALALKQASGDNVEAIFLLRA
YRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNV
EAAGFVSHLKLPHYVDFQAELELLKRLQQERNHG
>Mature_354_residues
MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELAALALKQASGDNVEAIFLLRA
YRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNV
EAAGFVSHLKLPHYVDFQAELELLKRLQQERNHG

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=354, Percent_Identity=98.8700564971751, Blast_Score=713, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 39010; Mature: 39010

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGP
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLAKFEEDSGAQPDDITR
CHHHHHHHHHEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
CCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEHHHHC
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG
CEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCC
EHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVDFQAELELLKRLQQERNHG
CCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGP
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLAKFEEDSGAQPDDITR
CHHHHHHHHHEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
CCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEHHHHC
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG
CEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCC
EHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVDFQAELELLKRLQQERNHG
CCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]