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The map label for this gene is ygdL [H]

Identifier: 170680315

GI number: 170680315

Start: 3028336

End: 3029136

Strand: Reverse

Name: ygdL [H]

Synonym: EcSMS35_2953

Alternate gene names: 170680315

Gene position: 3029136-3028336 (Counterclockwise)

Preceding gene: 170681128

Following gene: 170682370

Centisome position: 59.77

GC content: 54.43

Gene sequence:

>801_bases
GTGGTAATTAGTGATGCATGGCGTCAGCGTTTTGGTGGCACAGCGCGTCTGTATGGTGAAAAAGCATTGCAACTGTTTGC
TGACGCGCATATTTGTGTGGTTGGTATCGGTGGTGTCGGTTCCTGGGCGGCGGAAGCGCTGGCGCGCACGGGGATTGGCT
CAATCACGCTTATCGATATGGATGATGTGTGCGTCACCAATACCAATCGGCAAATTCATGCCCTGCGCGATAACGTTGGG
CTGGCAAAAGCGGAAGTTATGGCGGAGCGTATTCGCCAGATTAACCCGGAGTGCCGTGTAACGGTGGTGGATGATTTCGT
GACGCCGGATAACGTAGCGCAGTATATGAGCGTGGGTTATTCGTACGTGATTGATGCCATTGATAGTGTACGGCCCAAAG
CGGCGCTGATTGCTTATTGTCGGCGCAATAAAATCCCACTGGTCACAACCGGTGGCGCGGGTGGGCAGATTGACCCGACG
CAGATTCAGGTTACCGATCTGGCGAAAACGATTCAGGACCCGCTGGCGGCGAAGTTGCGTGAGCGCCTGAAAAGCGATTT
TGGCATAGTGAAAAACAGTAAAGGTAAGCTCGGCGTGGATTGCGTGTTCTCTACCGAAGCGCTGGTGTACCCGCAGTCAG
ATGGCACGGTCTGTGCAATGAAAGCTACGGCGGAAGGGCCAAAGCGGATGGATTGTGCATCTGGATTTGGTGCCGCGACG
ATGGTGACCGCCACCTTTGGTTTTGTTGCGGTTTCTCATGCGCTGAAGAAGATGATGGCGAAAGCGGCGCGTCAGGGGTA
A

Upstream 100 bases:

>100_bases
AACGTCTTTAGCGGCTGATGTGTTATTCTGTATACCGTTTTGACGGATACGACAGGGTGAGGGATAACCTCGCCCTTTTT
AATTCTGAGGTGCTATGTCT

Downstream 100 bases:

>100_bases
ACAATCAGGTCGGATGCGACGCAAACGTCCTTTCCGACCCGGTTCTGTGCTTAAACCTGCTTCGCCGCAGCGATAATCGC
CTCGCTTAACGCATTCAACC

Product: ThiF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGSITLIDMDDVCVTNTNRQIHALRDNVG
LAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIDPT
QIQVTDLAKTIQDPLAAKLRERLKSDFGIVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGAAT
MVTATFGFVAVSHALKKMMAKAARQG

Sequences:

>Translated_266_residues
MVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGSITLIDMDDVCVTNTNRQIHALRDNVG
LAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIDPT
QIQVTDLAKTIQDPLAAKLRERLKSDFGIVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGAAT
MVTATFGFVAVSHALKKMMAKAARQG
>Mature_266_residues
MVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGSITLIDMDDVCVTNTNRQIHALRDNVG
LAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIDPT
QIQVTDLAKTIQDPLAAKLRERLKSDFGIVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGAAT
MVTATFGFVAVSHALKKMMAKAARQG

Specific function: Unknown

COG id: COG1179

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI4885649, Length=143, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1789177, Length=266, Percent_Identity=98.8721804511278, Blast_Score=542, Evalue=1e-156,
Organism=Escherichia coli, GI87082356, Length=136, Percent_Identity=30.1470588235294, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787048, Length=131, Percent_Identity=32.824427480916, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=131, Percent_Identity=31.2977099236641, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321790, Length=205, Percent_Identity=25.3658536585366, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322825, Length=202, Percent_Identity=31.6831683168317, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24582879, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24660640, Length=137, Percent_Identity=29.9270072992701, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 28406; Mature: 28406

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGSITLIDM
CCCCHHHHHHCCCCEEHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEC
DDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSVGY
CCEEEECCCCEEEEHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
SYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIDPTQIQVTDLAKTIQDPLAAKLR
HHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
ERLKSDFGIVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGAAT
HHHHHHCCEEECCCCCCCEEEEEECCEEEEECCCCCEEEEEECCCCCCCCHHHCCCCHHH
MVTATFGFVAVSHALKKMMAKAARQG
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGSITLIDM
CCCCHHHHHHCCCCEEHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEC
DDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSVGY
CCEEEECCCCEEEEHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
SYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIDPTQIQVTDLAKTIQDPLAAKLR
HHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
ERLKSDFGIVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGAAT
HHHHHHCCEEECCCCCCCEEEEEECCEEEEECCCCCEEEEEECCCCCCCCHHHCCCCHHH
MVTATFGFVAVSHALKKMMAKAARQG
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]