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The map label for this gene is cmtB [H]

Identifier: 170680302

GI number: 170680302

Start: 3163865

End: 3164308

Strand: Reverse

Name: cmtB [H]

Synonym: EcSMS35_3075

Alternate gene names: 170680302

Gene position: 3164308-3163865 (Counterclockwise)

Preceding gene: 170681853

Following gene: 170683729

Centisome position: 62.43

GC content: 42.34

Gene sequence:

>444_bases
ATGCGGCTTATTAATTATTTTCCAGAATCATCAATTTCAGTTATACATTCAGCAAAAGACTGGCAGGAAGCTATCGATTT
CTCGATGGCATCATTGCTGGATAAAAACTATATCAGCGAGAATTACATTCAGGCTATTAAAGATTCCACCATGAGTAATG
GCCCTTACTATATTCTCGCACCAGGCGTGGCAATGCCTCATGCGCGACCAGAATGTGGGGCGCTTAAAACCGGGATGTCT
TTAACGTTACTTAAACAAGGCGTTTATTTTCCGGGGAATGACGAACCAATTAAATTACTCATCGGACTCTCTGCTGCCGA
TGCCGATTCGCACATTGGCGCTATTCAGGCGTTAAGTGAATTACTGTGCGAAGAAGAAATACTCGAACAACTCTTAACAG
CATCATCAGAAAAACAATTAGCGGACATTATCAGCCGCGGATAA

Upstream 100 bases:

>100_bases
GGGTTTTTTCTTCTCGCGTGCCCAATTGCTTTCATTAAACCGCACGAGTATTTTATTTTTACCGCCGAACGGTACCCTAA
AAATAAAATAAGGATAATTT

Downstream 100 bases:

>100_bases
TTACTCTCTCCTCTACAGGATAATATTATGGAAAACAAGTCTGCTCGTGCAAAGGTCCAGGCTTTTGGGGGCTTTTTGAC
GGCAATGGTCATCCCCAATA

Product: putative mannitol phosphotransferase subunit EIIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MRLINYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQAIKDSTMSNGPYYILAPGVAMPHARPECGALKTGMS
LTLLKQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG

Sequences:

>Translated_147_residues
MRLINYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQAIKDSTMSNGPYYILAPGVAMPHARPECGALKTGMS
LTLLKQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG
>Mature_147_residues
MRLINYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQAIKDSTMSNGPYYILAPGVAMPHARPECGALKTGMS
LTLLKQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789302, Length=147, Percent_Identity=95.9183673469388, Blast_Score=255, Evalue=7e-70,
Organism=Escherichia coli, GI2367359, Length=150, Percent_Identity=37.3333333333333, Blast_Score=103, Evalue=4e-24,
Organism=Escherichia coli, GI1790755, Length=132, Percent_Identity=37.1212121212121, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16034; Mature: 16034

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLINYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQAIKDSTMSNGPYYILA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
PGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSE
CCCCCCCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LLCEEEILEQLLTASSEKQLADIISRG
HHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRLINYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQAIKDSTMSNGPYYILA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
PGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSE
CCCCCCCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LLCEEEILEQLLTASSEKQLADIISRG
HHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]