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The map label for this gene is alsK [H]

Identifier: 170680209

GI number: 170680209

Start: 4652478

End: 4653407

Strand: Reverse

Name: alsK [H]

Synonym: EcSMS35_4550

Alternate gene names: 170680209

Gene position: 4653407-4652478 (Counterclockwise)

Preceding gene: 170679730

Following gene: 170680012

Centisome position: 91.81

GC content: 54.09

Gene sequence:

>930_bases
ATGCAAAAACAGCATAACGTCGTAGCGGGCGTGGATATGGGGGCAACGCATATCCGCTTTTGTCTGCGGACAGCAGAAGG
TGAAACGCTACACTGCGAAAAAAAGCGGACCGCAGAAGTTATTGCTCCCGACCTGGTGTCGGGTATCGGCGAAATGATTG
ACGAGCAACTCAGGCGCTTTAACGCTCGCTGTCGTGGTCTGGTGATGGGATTTCCGGCGCTGGTCAGTAAAGATAAACGC
ACCATTATTTCTACGCCTAACCTGCCGTTAACAGCGGCGGATTTATATGATCTCGCCGATAAGCTCGAAAATACGCTGAA
TTGTCCGGTTGAGTTTTCCCGCGACGTTAACCTGCAACTCTCCTGGGACGTAGTAGAAAACAGCCTTACGCAACAACTGG
TTCTGGCGGCCTATCTCGGTACGGGGATGGGGTTCGCAGTGTGGATGAACGGTGCGCCGTGGACGGGTGCACACGGTGTG
GCAGGCGAACTGGGCCATATCCCCCTGGGAGATATGACCCAACACTGCGCGTGTGGCAATCCTGGGTGCCTGGAAACCAA
TTGCTCTGGAATGGCGCTAAGACGCTGGTACGAACAACAGCCCCGAAATTACCCATTGAGCGCTCTTTTCGTCCATGCGG
AAAACGCCCCTTTCGTCCAGAGTCTGCTTGAAAACGCGGCACGAGCCATTGCCACCAGCATTAATCTGTTCGATCCCGAT
GCGGTAATTCTGGGTGGTGGCGTGATGGATATGCCCACCTTCCCACGCGAGACTCTCATTGCCATGACCCAAAAGTACCT
GCGCCGTCCACTGCCGTATCAGGTCGTGCGCTTTATTGCCGCCTCATCTTCTGACTTTAATGGCGCTCAGGGTGCAGCAA
TATTGGCACATCAACGTTTTTTGCCACAGTCCTGTGCTAAAGCCCCATGA

Upstream 100 bases:

>100_bases
GTCGGCACTTCCGGCCTGTTTAATCATGCGGAAAATATCGACGAAGCATGGAGAATTATGACCGCGCAGATTCTGGCTGC
AAAAAGCGAGGTACAGCCTC

Downstream 100 bases:

>100_bases
GTAAAGCGTTGGCACCGTAACGCAATGTCCCGCTGTGCCAACGACAAGTACAATATATATAATAAACACATAAAACAGCT
CATATTATTTTCAGCAATGA

Product: D-allose kinase

Products: NA

Alternate protein names: Allokinase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPDLVSGIGEMIDEQLRRFNARCRGLVMGFPALVSKDKR
TIISTPNLPLTAADLYDLADKLENTLNCPVEFSRDVNLQLSWDVVENSLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSALFVHAENAPFVQSLLENAARAIATSINLFDPD
AVILGGGVMDMPTFPRETLIAMTQKYLRRPLPYQVVRFIAASSSDFNGAQGAAILAHQRFLPQSCAKAP

Sequences:

>Translated_309_residues
MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPDLVSGIGEMIDEQLRRFNARCRGLVMGFPALVSKDKR
TIISTPNLPLTAADLYDLADKLENTLNCPVEFSRDVNLQLSWDVVENSLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSALFVHAENAPFVQSLLENAARAIATSINLFDPD
AVILGGGVMDMPTFPRETLIAMTQKYLRRPLPYQVVRFIAASSSDFNGAQGAAILAHQRFLPQSCAKAP
>Mature_309_residues
MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPDLVSGIGEMIDEQLRRFNARCRGLVMGFPALVSKDKR
TIISTPNLPLTAADLYDLADKLENTLNCPVEFSRDVNLQLSWDVVENSLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSALFVHAENAPFVQSLLENAARAIATSINLFDPD
AVILGGGVMDMPTFPRETLIAMTQKYLRRPLPYQVVRFIAASSSDFNGAQGAAILAHQRFLPQSCAKAP

Specific function: Catalyzes the phosphorylation of D-allose to D-allose 6- P. Has also low level glucokinase activity in vitro [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI1790522, Length=309, Percent_Identity=97.0873786407767, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1787363, Length=309, Percent_Identity=29.4498381877023, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081733, Length=308, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786891, Length=289, Percent_Identity=25.2595155709343, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.55 [H]

Molecular weight: Translated: 33726; Mature: 33726

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPDLVSGIGEMIDEQLRRF
CCCCCCEEEECCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NARCRGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKLENTLNCPVEFSRDVNLQL
HHHHHHHHCCCHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCEEECCCCCEEE
SWDVVENSLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGVAGELGHIPLGDMTQHCACGN
EHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
PGCLETNCSGMALRRWYEQQPRNYPLSALFVHAENAPFVQSLLENAARAIATSINLFDPD
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
AVILGGGVMDMPTFPRETLIAMTQKYLRRPLPYQVVRFIAASSSDFNGAQGAAILAHQRF
EEEECCCEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LPQSCAKAP
CHHHHCCCC
>Mature Secondary Structure
MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPDLVSGIGEMIDEQLRRF
CCCCCCEEEECCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NARCRGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKLENTLNCPVEFSRDVNLQL
HHHHHHHHCCCHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCEEECCCCCEEE
SWDVVENSLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGVAGELGHIPLGDMTQHCACGN
EHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
PGCLETNCSGMALRRWYEQQPRNYPLSALFVHAENAPFVQSLLENAARAIATSINLFDPD
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
AVILGGGVMDMPTFPRETLIAMTQKYLRRPLPYQVVRFIAASSSDFNGAQGAAILAHQRF
EEEECCCEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LPQSCAKAP
CHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 9401019 [H]