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The map label for this gene is ybeF [H]

Identifier: 170679981

GI number: 170679981

Start: 661027

End: 661980

Strand: Reverse

Name: ybeF [H]

Synonym: EcSMS35_0649

Alternate gene names: 170679981

Gene position: 661980-661027 (Counterclockwise)

Preceding gene: 170683874

Following gene: 170681300

Centisome position: 13.06

GC content: 44.97

Gene sequence:

>954_bases
GTGGATAGTAATAATCAAATTGAAACCTGTTTAAGCAGAAAGTCGTCAGAAGGTAAACCACAAATATTTACAACCTTACG
AAATATTGATCTTAACCTTCTGACTATTTTTGAAGCTGTATATGTACATAAAGGGATCGTTAATGCAGCGAAAGTGCTTA
ATCTGACACCCTCGGCAATCAGTCAGTCTATTCAGAAACTGCGCGTTATATTCCCTGACCCATTGTTTATTCGCAAAGGC
CAGGGTGTCACTCCTACCGCATTTGCGATGCATCTACATGAGTATATCAGTCAGGGCCTTGAGTCCATTCTTGGCGCGCT
GGATATCGAAGGAAGCTATGATAAGCAACGAACGATAACTATTGCGACCACCCCCTCGGTCGGAGCCCTGGTCCTTCCTG
TCATCTATCGGGCGATTAAATCTCACTATCCGCAGCTTTTACTGCGCAACCCACCCACCAGCGACGCGGAAAACCAACTC
AGTCAGTTTCAAACCGATCTCATCATCGATAACATGTTTTGCACCAATCGTACGGTGCAACATCATGTTCTGTTCACCGA
CAATATGGTGTTAATTTGCCGTGAGGGAAATCCACTACTCTCTTTAGAAGATGACAGAGAGACTATCGACAACGCTGCGC
ATATACTCCTGTTACCGGAAGGGCAAAATTTCAGCGGTCTGCGGCAGAGAGTTCAAGAGATGTTTCCGGACCGGCAAATC
AGTTTCACCAGCTACAACATTTTGACAATCGCTGCACTGGTTGCCAACAGTGACATGTTAGCGATTATTCCCAGCCGTTT
TTATAACCTGTTTAGCCGTTGTTGGCCGCTAGAAAAACTGCCTTTTCCGTCCTTAAATGAGGAGCAAATAGATTTCTCTA
TCCACTACAACAAATTCAGCCTGCGTGATCCCATCCTGCACGGGGTAATAGATGTTATCCGGAATGCATTTTGA

Upstream 100 bases:

>100_bases
GTTGATTATGGTGAGCAAAAGCGTTCCGTTCCAATTAGACATAAGATTATTGTAATTTTTTTTAATATTATTTTTCCATG
AATAAAATTGGAGTAAGTGC

Downstream 100 bases:

>100_bases
AAAAGTATTGTTACAGCCAGATCACAGCGAAGAACGAAAAAATGGCACAATCCAACAACAATTTTACATTTTCGCGACCG
CTTTGGCTGCTTTCAGGTCC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MDSNNQIETCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKSHYPQLLLRNPPTSDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLLPEGQNFSGLRQRVQEMFPDRQI
SFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF

Sequences:

>Translated_317_residues
MDSNNQIETCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKSHYPQLLLRNPPTSDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLLPEGQNFSGLRQRVQEMFPDRQI
SFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF
>Mature_317_residues
MDSNNQIETCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKSHYPQLLLRNPPTSDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLLPEGQNFSGLRQRVQEMFPDRQI
SFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081766, Length=317, Percent_Identity=98.1072555205047, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1786820, Length=302, Percent_Identity=30.1324503311258, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI87081684, Length=272, Percent_Identity=26.4705882352941, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790147, Length=296, Percent_Identity=23.6486486486486, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=245, Percent_Identity=22.8571428571429, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35854; Mature: 35854

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSNNQIETCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAI
CCCCHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTIT
HHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IATTPSVGALVLPVIYRAIKSHYPQLLLRNPPTSDAENQLSQFQTDLIIDNMFCTNRTVQ
EEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCHHHCCCCEE
HHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLLPEGQNFSGLRQRVQEMFPDRQI
EEEEEECCEEEEEECCCCEEEECCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCEE
SFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFS
EEECHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECEEC
LRDPILHGVIDVIRNAF
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSNNQIETCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAI
CCCCHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTIT
HHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IATTPSVGALVLPVIYRAIKSHYPQLLLRNPPTSDAENQLSQFQTDLIIDNMFCTNRTVQ
EEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCHHHCCCCEE
HHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLLPEGQNFSGLRQRVQEMFPDRQI
EEEEEECCEEEEEECCCCEEEECCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCEE
SFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFS
EEECHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECEEC
LRDPILHGVIDVIRNAF
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8444795; 8905232; 9278503 [H]