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The map label for this gene is yihQ [H]

Identifier: 170679942

GI number: 170679942

Start: 4361872

End: 4363908

Strand: Reverse

Name: yihQ [H]

Synonym: EcSMS35_4263

Alternate gene names: 170679942

Gene position: 4363908-4361872 (Counterclockwise)

Preceding gene: 170683996

Following gene: 170680543

Centisome position: 86.1

GC content: 53.8

Gene sequence:

>2037_bases
ATGGATACGCCACGCCCACAGCTATTAGATTTTCAATTTCATCAGAATAACGACAGTTTTACTCTACGTTTTCAAGACCG
CCTTATTTTAATCCATAGCAAAGATAATCCTTGCTTATCGATTGGCTCAGGTATAGCGGATATCGATATGTTCCGCGGTA
ATTTCAGCATTAAAGATAAATTGCAGGAGAAAATTGCGCTTACCGACGCCATCGTCAGCCAGTCACCGGATGGTTGGTTA
ATCCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGCGTATCTCTGCCGACGATCAGGGCCGTTTATTGCTGGAACT
ACAAAACGACAACCATCACCACAACCGCATCTGGCTGCGTCTTGCCGCACAACCAGAGGACCATATCTACGGCTGCGGCG
AACAATTCTCCTACTTTGATCTGCGCGGCAAACCGTTCCCGCTATGGACCAGTGAACAAGGCGTTGGTCGCAACAAACAA
ACCTATGTCACCTGGCAGGCCGACTGCAAAGAGAACGCGGGCGGCGACTATTACTGGACTTTCTTCCCGCAGCCTACGTT
TGTCAGCACGCAGAAGTATTACTGCCATGTTGATAACAGTTGCTATATGAACTTCGACTTTAGTGCCCCGGAATACCATG
AACTGGCGCTGTGGGAAGAGAAAGCAACACTGCGTTTTGAATGTGCTGACACATACATCTCCCTGTTAGAAAAATTAACC
GCCCTGCTGGGACGCCAGCCAGAACTGCCCGACTGGATTTATGACGGGGTAACGCTCGGCATTCAGGGCGGGACTGAAGT
GTGCCAGAAGAAACTGGACACCATGCGTAACGCGGGCGTGAAGGTCAACGGCATCTGGGCGCAGGACTGGTCCGGGATCC
GTATGACCTCGTTTGGTAAACGTGTGATGTGGAACTGGAAGTGGAACAGCGAAAACTATCCGCAACTGGATTCGCGTATT
AAACAGTGGAACAAAGAAGGCGTGCAGTTCCTCGCCTACATCAACCCGTATGTTGCCAGCGATAAGGACCTCTGCGCCGA
GGCGGCTTCACACGGCTATCTGGCAAAAGATGCCTCTGGCGGCGACTATCTGGTGGAGTTTGGCGAGTTCTATGGCGGCG
TTGTCGATCTCACCAATCCAGAAGCCTACGCCTGGTTCAAGGAAGTGATCAAAAAGAACATGATTGAACTTGGCTGCGGC
GGCTGGATGGCTGACTTCGGCGAGTATCTGCCCACCGACACGTACCTGCATAACGGCGTCAGCGCGGAAATTATGCATAA
CGCCTGGCCTGCGCTGTGGGCGAAATGTAACTACGAAGCCCTTGAAGAAACAGGCAAGCTCGGCGAGCTCCTCTTCTTTA
TGCGCGCCGGTTCTACCGGTAGCCAGAAATACTCCACCATGATGTGGGCGGGCGACCAGAACGTTGACTGGAGTCTGGAC
GATGGACTGGCGTCAGTCGTCCCGGCGGCGCTGTCTCTGGCAATGACCGGACATGGCCTGCATCACAGTGATATTGGCGG
TTACACCACCCTGTTTGAGATGAAGCGCAGCAAAGAGCTGCTGCTGCGCTGGTGCGATTTCAGCGCCTTCACGCCGATGA
TGCGCACCCACGAAGGTAACCGTCCTGGCGACAACTGGCAGTTTGATAGCGACGCGGAAACTATCGCCCATTTTGCCCGT
ATGACCACCGTCTTCACCACCCTGAAACCTTACCTGAAAGAGGCCGTCGCGCTGAATGCGAAGTCCGGCCTGCCGGTTAT
GCGCCCGCTGTTCCTGCATTACGAAGACGACGCGCAGACCTACACCCTGAAATATCAGTACCTGTTAGGCCGCGACATTC
TGGTCGCTCCGGTGCATGAAGAAGGCCGTAGCGACTGGACGCTCTATCTGCCGGAGGATAACTGGGTCCATGCCTGGACG
GGTGAAGCGTTCCGGGGCGGGGAAGTTACCGTTAATGCGCCCATCGGTAAACCGCCGGTCTTTTATCGCGCCGGTAGCGA
ATGGGCGGCACTGTTCGCCACGTTAAAAAGTATCTAA

Upstream 100 bases:

>100_bases
CTGTAAAGTTTATATATATCAGTTTTATGAGAATCATTTTACATAAGCCTGTAATAACGGGAAGGAAACAATCTCGTCCT
CAACTCTATGGAGATTATTT

Downstream 100 bases:

>100_bases
TCATGTCCGCCCGTGGGGAAACTCACGGGCAAAGGGAGAACAACGATGAGTCACATCACAACGGAAGATCCAGCAACTTT
ACGCCTGCCCTTTAAAGAGA

Product: alpha-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLSIGSGIADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWL
IHFSRGSDISATLRISADDQGRLLLELQNDNHHHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEEKATLRFECADTYISLLEKLT
ALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRI
KQWNKEGVQFLAYINPYVASDKDLCAEAASHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCG
GWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGELLFFMRAGSTGSQKYSTMMWAGDQNVDWSLD
DGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDSDAETIAHFAR
MTTVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNWVHAWT
GEAFRGGEVTVNAPIGKPPVFYRAGSEWAALFATLKSI

Sequences:

>Translated_678_residues
MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLSIGSGIADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWL
IHFSRGSDISATLRISADDQGRLLLELQNDNHHHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEEKATLRFECADTYISLLEKLT
ALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRI
KQWNKEGVQFLAYINPYVASDKDLCAEAASHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCG
GWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGELLFFMRAGSTGSQKYSTMMWAGDQNVDWSLD
DGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDSDAETIAHFAR
MTTVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNWVHAWT
GEAFRGGEVTVNAPIGKPPVFYRAGSEWAALFATLKSI
>Mature_678_residues
MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLSIGSGIADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWL
IHFSRGSDISATLRISADDQGRLLLELQNDNHHHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEEKATLRFECADTYISLLEKLT
ALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRI
KQWNKEGVQFLAYINPYVASDKDLCAEAASHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCG
GWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGELLFFMRAGSTGSQKYSTMMWAGDQNVDWSLD
DGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDSDAETIAHFAR
MTTVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNWVHAWT
GEAFRGGEVTVNAPIGKPPVFYRAGSEWAALFATLKSI

Specific function: Exhibits hydrolysis activity against alpha-glucosyl fluoride, although natural substrates, such as alpha-glucobioses are scarcely hydrolyzed [H]

COG id: COG1501

COG function: function code G; Alpha-glucosidases, family 31 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 31 family [H]

Homologues:

Organism=Homo sapiens, GI153791946, Length=349, Percent_Identity=24.6418338108883, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI88900491, Length=388, Percent_Identity=25.5154639175258, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI38202257, Length=388, Percent_Identity=25.5154639175258, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI66346737, Length=397, Percent_Identity=25.6926952141058, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI119393895, Length=407, Percent_Identity=22.8501228501229, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI119393893, Length=407, Percent_Identity=22.8501228501229, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI119393891, Length=407, Percent_Identity=22.8501228501229, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI221316699, Length=242, Percent_Identity=27.6859504132231, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI310115361, Length=246, Percent_Identity=28.4552845528455, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI2367323, Length=678, Percent_Identity=97.6401179941003, Blast_Score=1386, Evalue=0.0,
Organism=Escherichia coli, GI2367256, Length=550, Percent_Identity=24.7272727272727, Blast_Score=178, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17560800, Length=382, Percent_Identity=22.7748691099476, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17560798, Length=382, Percent_Identity=22.7748691099476, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71991189, Length=379, Percent_Identity=24.2744063324538, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985706, Length=238, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32563849, Length=231, Percent_Identity=30.7359307359307, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319706, Length=385, Percent_Identity=22.3376623376623, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650054, Length=346, Percent_Identity=26.0115606936416, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24643749, Length=400, Percent_Identity=24.5, Blast_Score=84, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24643753, Length=400, Percent_Identity=24.5, Blast_Score=84, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24643751, Length=400, Percent_Identity=24.5, Blast_Score=84, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24643746, Length=400, Percent_Identity=24.5, Blast_Score=84, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357605, Length=400, Percent_Identity=24.5, Blast_Score=84, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571440, Length=379, Percent_Identity=21.1081794195251, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571438, Length=379, Percent_Identity=21.1081794195251, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011013
- InterPro:   IPR000322
- InterPro:   IPR017853 [H]

Pfam domain/function: PF01055 Glyco_hydro_31 [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 77176; Mature: 77176

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLSIGSGIADIDMFRGNFSIKDK
CCCCCCCEEEEEEECCCCEEEEEEECEEEEEECCCCCEEEECCCCCEEEEECCCCCHHHH
LQEKIALTDAIVSQSPDGWLIHFSRGSDISATLRISADDQGRLLLELQNDNHHHNRIWLR
HHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEE
LAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQADCKENAGGDYYWT
EECCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEE
FFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEEKATLRFECADTYISLLEKLT
ECCCCCEEECCEEEEEECCEEEEEECCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHH
ALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSFGK
HHHCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCEEECCCC
RVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYINPYVASDKDLCAEAASHGYLAKDASG
EEEEEEEECCCCCCHHHHHHHHHCHHHEEEEEEECCCCCCCHHHHHHHHHCCCEEECCCC
GDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCGGWMADFGEYLPTDTYLHNGV
CHHEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCHHHHCCC
SAEIMHNAWPALWAKCNYEALEETGKLGELLFFMRAGSTGSQKYSTMMWAGDQNVDWSLD
CHHHHHCCCHHHHHCCCHHHHHHCCCHHHEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
DGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGN
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCC
RPGDNWQFDSDAETIAHFARMTTVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEECCCEE
YTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPV
EEEEEEEEECCCEEEEECCCCCCCCEEEECCCCCEEEEECCCCCCCCEEEEECCCCCCCE
FYRAGSEWAALFATLKSI
EEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLSIGSGIADIDMFRGNFSIKDK
CCCCCCCEEEEEEECCCCEEEEEEECEEEEEECCCCCEEEECCCCCEEEEECCCCCHHHH
LQEKIALTDAIVSQSPDGWLIHFSRGSDISATLRISADDQGRLLLELQNDNHHHNRIWLR
HHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEE
LAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQADCKENAGGDYYWT
EECCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEE
FFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEEKATLRFECADTYISLLEKLT
ECCCCCEEECCEEEEEECCEEEEEECCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHH
ALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSFGK
HHHCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCEEECCCC
RVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYINPYVASDKDLCAEAASHGYLAKDASG
EEEEEEEECCCCCCHHHHHHHHHCHHHEEEEEEECCCCCCCHHHHHHHHHCCCEEECCCC
GDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCGGWMADFGEYLPTDTYLHNGV
CHHEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCHHHHCCC
SAEIMHNAWPALWAKCNYEALEETGKLGELLFFMRAGSTGSQKYSTMMWAGDQNVDWSLD
CHHHHHCCCHHHHHCCCHHHHHHCCCHHHEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
DGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGN
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCC
RPGDNWQFDSDAETIAHFARMTTVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEECCCEE
YTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPV
EEEEEEEEECCCEEEEECCCCCCCCEEEECCCCCEEEEECCCCCCCCEEEEECCCCCCCE
FYRAGSEWAALFATLKSI
EEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503 [H]