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The map label for this gene is cysP [H]

Identifier: 170679837

GI number: 170679837

Start: 2633981

End: 2634997

Strand: Reverse

Name: cysP [H]

Synonym: EcSMS35_2580

Alternate gene names: 170679837

Gene position: 2634997-2633981 (Counterclockwise)

Preceding gene: 170683796

Following gene: 170681836

Centisome position: 51.99

GC content: 53.59

Gene sequence:

>1017_bases
ATGGCCGTTAACTTACTGAAAAAGAACTCACTCGCGCTGGTCGCTTCTCTGCTGCTGGCGGGCCATGTACAGGCAACGGA
ACTGCTGAACAGTTCTTATGACGTCTCCCGCGAGCTGTTTGCCGCCCTGAATCCGCCGTTTGAGCAACAATGGGCAAAAG
ATAACGGCGGCGACAAACTGACGATAAAACAATCTCATGCCGGATCATCAAAACAGGCGCTGGCGATTTTACAGGGCTTA
AAAGCCGACGTTGTCACTTATAACCAGGTGACCGACGTACAAATCCTGCACGATAAAGGCAAGCTGATCCCGGCCGACTG
GCAGTCGCGCCTGCCGAATAATAGCTCGCCGTTCTACTCCACCATGGGTTTCCTGGTACGTAAGGGCAACCCGAAGAATA
TCCACGACTGGAACGATCTGGTGCGCTCCGACGTGAAGCTGATTTTCCCGAACCCAAAAACCTCCGGTAACGCGCGTTAT
ACCTATCTGGCGGCATGGGGCGCAGCGGATAAAGCTGACGGTGGCGACAAAGCCAAAACCGAACAGTTTATGACCCAGTT
CCTGAAAAACGTTGAAGTGTTCGATACTGGCGGTCGTGGCGCGACCACCACTTTTGCCGAGCGCGGCCTGGGCGATGTGC
TGATCAGCTTCGAGTCGGAAGTGAACAACATCCGCAAACAGTATGAAGCGCAGGGCTTTGAAGTGGTGATTCCGAAAACC
AATATTCTGGCGGAATTCCCGGTGGCGTGGGTCGATAAAAACGTGCAGGCCAACGGTACGGAAAAAGCAGCAAAAGCTTA
CCTGAACTGGCTCTACAGCCCGCAGGCGCAAACCATCATCACCGACTATTACTACCGCGTAAATAACCCGGAGGTGATGG
ACAAACTGAAAGACAAATTCCCGCAGACCGAACTGTTCCGCGTGGAAGACAAATTTGGCTCCTGGCCGGAAGTGATGAAA
ACCCACTTCACCAGCGGCGGCGAGTTAGACAAGCTGTTAGCGGCGGGGCGTAACTGA

Upstream 100 bases:

>100_bases
ACTTCCAAATCACCAAACGGTATATAAAACCGTTACTCCTTTCACGTCCGTTATAAATATGATGGCTATTAGAAAGTCAT
TTAATTAATAAGGGTGCGCA

Downstream 100 bases:

>100_bases
TGTTTGCAGTTTCATCAAGACGCGTGCTGCCGGGCTTTACCTTAAGCCTCGGCACCAGTCTGCTGTTTGTTTGCCTGATT
TTGCTGCTGCCGCTCTCCGC

Product: thiosulfate transporter subunit

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGL
KADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMK
THFTSGGELDKLLAAGRN

Sequences:

>Translated_338_residues
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGL
KADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMK
THFTSGGELDKLLAAGRN
>Mature_337_residues
AVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLK
ADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYT
YLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKTN
ILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKT
HFTSGGELDKLLAAGRN

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=338, Percent_Identity=99.7041420118343, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI1790351, Length=323, Percent_Identity=46.4396284829721, Blast_Score=301, Evalue=4e-83,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37629; Mature: 37498

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00092 N6_MTASE ; PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKL
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEE
TIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYS
EEECCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCCHHHCCCCCCCCHHH
TMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYTYLAAWGAADKADGGDKAKT
HHHHHEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHH
EQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
HHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCC
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKF
CCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHC
PQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLAAGRN
CCCCEEEEHHHCCCCHHHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
AVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKL
CCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEE
TIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYS
EEECCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCCHHHCCCCCCCCHHH
TMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYTYLAAWGAADKADGGDKAKT
HHHHHEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHH
EQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
HHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCC
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKF
CCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHC
PQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLAAGRN
CCCCEEEEHHHCCCCHHHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]