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The map label for this gene is lfiN [H]

Identifier: 170679793

GI number: 170679793

Start: 260200

End: 260571

Strand: Reverse

Name: lfiN [H]

Synonym: EcSMS35_0246

Alternate gene names: 170679793

Gene position: 260571-260200 (Counterclockwise)

Preceding gene: 170680472

Following gene: 170684182

Centisome position: 5.14

GC content: 54.84

Gene sequence:

>372_bases
ATGAGTAAACAAGACGATATTTTAGCCGGGGATTTTGGCCTCGCCGATGAAGTTGCGCCTGTCACGAAATCCGCCGACGC
CGAAACGCTGGTCACCCGCCTGGAAGACCGCTTTTCCGACTCGATGACGCTGCTGAAACGCATTCCGGTGACCTTAACGC
TGGAAGTGTCGTCGGTGGAAATTATGTTAGCCGACCTGCTTAACATCGACGACGACACGGTGATTGAGCTGGATAAGCTC
GCCGGGGAGCCGCTGGATATCAAAGTGAACAATATCCTGCTGGGCAAAGCGGAAGTGGTGGTGGTCAACGAGAAGTACGG
CCTGCGCGTGCTGGAGTTTAACACCCGCGATATCAACGACCTGGCACCATGA

Upstream 100 bases:

>100_bases
CCGGTGGCGATTGGGCAATCTGAATTATTTAGCGCCCTGATTGTGGAAGACAAAGACAAACTGTTTTTGTCGGAGCTGGC
GGGCAAGAATGAGAATAGCC

Downstream 100 bases:

>100_bases
AACGCCGCACTCAACTGGCGCTGGGCCTTGGGCTGCTGGCGCTTGCGCCGCTGGCGTTGGCCCAGGGTGGCGATATTGCG
CTACTTAACGTGGTGACCCA

Product: lateral flagellar export/assembly protein LfiN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MSKQDDILAGDFGLADEVAPVTKSADAETLVTRLEDRFSDSMTLLKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKL
AGEPLDIKVNNILLGKAEVVVVNEKYGLRVLEFNTRDINDLAP

Sequences:

>Translated_123_residues
MSKQDDILAGDFGLADEVAPVTKSADAETLVTRLEDRFSDSMTLLKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKL
AGEPLDIKVNNILLGKAEVVVVNEKYGLRVLEFNTRDINDLAP
>Mature_122_residues
SKQDDILAGDFGLADEVAPVTKSADAETLVTRLEDRFSDSMTLLKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLA
GEPLDIKVNNILLGKAEVVVVNEKYGLRVLEFNTRDINDLAP

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Bacterial flagellum basal body (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=72, Percent_Identity=44.4444444444444, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 13514; Mature: 13382

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQDDILAGDFGLADEVAPVTKSADAETLVTRLEDRFSDSMTLLKRIPVTLTLEVSSVE
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHE
IMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVVNEKYGLRVLEFNTRDIND
EEEEHHHCCCCCCEEEEHHHCCCCCEEEECEEEECCEEEEEEECCCCEEEEEECCCCCCC
LAP
CCC
>Mature Secondary Structure 
SKQDDILAGDFGLADEVAPVTKSADAETLVTRLEDRFSDSMTLLKRIPVTLTLEVSSVE
CCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHE
IMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVVNEKYGLRVLEFNTRDIND
EEEEHHHCCCCCCEEEEHHHCCCCCEEEECEEEECCEEEEEEECCCCEEEEEECCCCCCC
LAP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622217; 10984043 [H]