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The map label for this gene is hsrA

Identifier: 170679741

GI number: 170679741

Start: 4218984

End: 4220411

Strand: Reverse

Name: hsrA

Synonym: EcSMS35_4121

Alternate gene names: 170679741

Gene position: 4220411-4218984 (Counterclockwise)

Preceding gene: 170681987

Following gene: 170679686

Centisome position: 83.27

GC content: 49.44

Gene sequence:

>1428_bases
ATGAGCGATAAAAAGAAGCGCAGTATGGCGGGTTTGCCGTGGATCGCGGCGATGGCCTTCTTCATGCAGGCACTTGATGC
CACTATTCTGAATACCGCCTTACCCGCAATCGCTCATAGCCTTAATCGTTCTCCTCTCGCGATGCAATCAGCCATCATCA
GTTATACGCTGACGGTGGCGATGCTTATTCCGGTAAGCGGATGGCTAGCCGATCGCTTCGGTACGCGTCGCATATTTACC
CTTGCCGTGAGTCTGTTCACGTTGGGTTCTCTGGCCTGCGCACTTTCTAACTCGCTACCACAGCTGGTTGTCTTCCGGGT
TATTCAAGGGATAGGCGGCGCAATGATGATGCCTGTTGCTCGGCTGGCCTTACTGCGCGCTTATCCTCGTAATGAGCTTC
TTCCTGTATTGAATTTTGTCGCCATGCCGGGTCTGGTGGGGCCAATTTTAGGCCCCGTTCTTGGCGGCGTGCTGGTCACC
TGGGCAACCTGGCACTGGATATTTTTAATCAATATCCCCATAGGTATTGCGGGCCTTCTTTACGCGCGCAAACATATGCC
CAATTTCACCACCGCACGACGCAGATTCGATATCACTGGCTTTTTGCTGTTTGGCCTTAGCCTTGTTCTCTTCTCAAGCG
GAATAGAGCTATTCGGGGAAAAGATTGTCGCCAGTTGGATTGCCTTGACGGTAATTGTCACCAGCATCGGGTTACTGCTT
CTCTATATTCTCCATGCACGACGCACGCCAAACCCATTAATTTCATTAGATTTATTTAAAACCCGCACTTTCTCGATCGG
TATCGTAGGCAATATTGCAACCCGTCTGGGGACCGGTTGTGTACCGTTCCTTATGCCATTGATGTTACAGGTAGGATTTG
GTTATCAGGCGTTTATTGCCGGCTGTATGATGGCACCGACAGCGTTAGGTTCCATTATTGCAAAATCGATGGTTACCCAA
GTCTTACGTCGTCTGGGCTATCGCCATACGTTAGTGGGGATCACGGTGATTATTGGGCTAATGATCGCTCAGTTCTCTTT
GCAATCACCGGCAATGGCGATATGGATGCTGATCTTGCCGTTGTTTATATTAGGGATGGCTATGTCGACGCAATTTACCG
CGATGAATACCATCACACTTGCCGATCTGACCGATGACAACGCCAGCAGCGGTAACAGTGTTCTGGCGGTCACGCAGCAA
CTGTCTATCAGTTTAGGCGTTGCTGTAAGTGCGGCCGTCCTTCGCGTTTATGAAGGGATGGAAGGCACAACGACTGTCGA
ACAATTCCACTATACGTTTATCACGATGGGCATTATTACTGTTGCTTCAGCAGCAATGTTCATGCTTCTGAAAACAACCG
ATGGTAATAATTTGATCAAAAGACAGCGTAAATCTAAGCCGAACCGCGTTCCATCAGAATCGGAGTAA

Upstream 100 bases:

>100_bases
GTCGATGCGATTATCCAAAGCGATGGCGACGCGGCATTTAAAGCTTGTCAGGCGCTGCTACGCTCACCAGATAAGTGATA
ACCCGATAACAGGACATTGA

Downstream 100 bases:

>100_bases
GTTGTAATCGTTGTTGCTGAAGGGTCGGTTGGGTTATCCGATGGATGAGTACATCAATCGCCAGCTCCCCCAGTTCATCT
TTCGGTTGATGGATAGTGGT

Product: DHA2 family drug:H+ antiporter-1

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFT
LAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
LYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQ
VLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQ
LSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE

Sequences:

>Translated_475_residues
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFT
LAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
LYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQ
VLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQ
LSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE
>Mature_474_residues
SDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTL
AVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTW
ATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLLL
YILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQV
LRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQL
SISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1790195, Length=475, Percent_Identity=100, Blast_Score=949, Evalue=0.0,
Organism=Escherichia coli, GI1788392, Length=459, Percent_Identity=45.5337690631808, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789042, Length=426, Percent_Identity=32.1596244131455, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788710, Length=437, Percent_Identity=32.2654462242563, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=28.5024154589372, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=473, Percent_Identity=25.7928118393235, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=31.9727891156463, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=32.2834645669291, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=402, Percent_Identity=24.1293532338308, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSRA_ECOLI (P31474)

Other databases:

- EMBL:   L10328
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C65179
- RefSeq:   AP_004033.1
- RefSeq:   NP_418210.1
- ProteinModelPortal:   P31474
- DIP:   DIP-12470N
- MINT:   MINT-1239630
- STRING:   P31474
- EnsemblBacteria:   EBESCT00000004342
- EnsemblBacteria:   EBESCT00000014893
- GeneID:   948265
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3733
- KEGG:   eco:b3754
- EchoBASE:   EB1683
- EcoGene:   EG11732
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008825
- HOGENOM:   HBG679341
- OMA:   ITMGVIT
- ProtClustDB:   CLSK880776
- BioCyc:   EcoCyc:YIEO-MONOMER
- Genevestigator:   P31474
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 51463; Mature: 51331

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1d63db0c)-; HASH(0x1bfe4f68)-; HASH(0x1da691a4)-; HASH(0x18943388)-; HASH(0x1d1a0d90)-; HASH(0x1d95dec0)-; HASH(0x1da258cc)-; HASH(0x1cc32098)-; HASH(0x1dd78014)-; HASH(0x1cb81548)-; HASH(0x16924350)-; HASH(0x1daa7e04)-; HASH(0x1b0301d8)-; HASH(0x1d409724)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIFLINIPIGIAGLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHH
YARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIA
HHHHHHCCCCCCCEEEEEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
GCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRVYEGM
HHHHHHHHHCHHHHEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
EGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure 
SDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIFLINIPIGIAGLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHH
YARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIA
HHHHHHCCCCCCCEEEEEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
GCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRVYEGM
HHHHHHHHHCHHHHEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
EGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503