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The map label for this gene is spoT [H]

Identifier: 170079402

GI number: 170079402

Start: 2940458

End: 2942725

Strand: Reverse

Name: spoT [H]

Synonym: SYNPCC7002_A2816

Alternate gene names: 170079402

Gene position: 2942725-2940458 (Counterclockwise)

Preceding gene: 170079404

Following gene: 170079401

Centisome position: 97.83

GC content: 50.84

Gene sequence:

>2268_bases
ATGACTGCTTCTCCCGTTCAATTAACTGCCGCATCCGAAACAGAAAAATTTGATGTGCCGCTACCTGCTTGGTTGAAAAA
TTATCTCTATCAGCCAGAGCAATTGGTCGGGGTCTCCGAAGGCGATCGCCAACTCATCGGTGAAGCCTTTCGCTTTGCCC
ATGCCCTCCACGAAGGTCAAACCCGTAAATCTGGCGAGCCTTATATTGCCCATCCCATTGCCGTAGCAGGATTGTTGCTC
GATCTAGGCGGAAATGGGGCGATGATTGCGGCGGGATTTCTCCATGATGTGGTCGAAGACACAGAAGTCAGTCCCGAAGA
AATTGAAGAACGCTTTGGCGCTGAAACCCGCAAGCTTGTCGAAGGGGTAACCAAGCTCACCAAATTTACTTTTTCTAGCA
AAACAGAACGCCAAGCAGAAAATTTCCGGCGGATGTTCCTTTCCATGGCCGAGGATATCCGCGTGATCGTCGTGAAACTC
GCCGATCGCCTCCACAATATGCGCACCCTCGAACATCTGCGCCCCGACAAACAAGAACGCATTGCCCTGGAAACCCGCGA
AATCTTTGCGCCCCTGGCCAATCGCTTAGGGATTTGGCGTTTTAAGTGGGAACTAGAAGATCTCTGTTTTAAGTATCTCG
AGCGGGAAGCCTACCGTTCGATGCAAAAACACATTTCCGAAAAACGCACCGAGCGGGAAGCCAAACTCGAAGAGGCCATT
GAAATTATCCGCGATCGCCTGCGTCACCTGGGCCTCCACGTCTGGGAAATTAAAGGTCGCCCGAAGCACCTCTACAGCAT
CTATCACAAGATGCAACGACAACACAAAGAATTTGATGAAATCTTTGACATCGCTGGGATTCGGATTATTGTCGAAACCA
ATGACGAATGTTACCGCGCCCTGGCGGTGATTCACGATGCCTTTAAACCGATTCCTGGACGCTTTAAAGACTACATTGGC
CTGCCGAAGCCCAACCGCTACCAATCCCTCCACACCACCGTTGTCGGCTTAAATGGTCGCCCCCTCGAAGTGCAAATTCG
CACCATGGAAATGCACCACATCGCCGAATACGGGATTGCCGCCCACTGGAAATATAAAGAAGCCGGTCATTCCGCTGCTA
GTTTCACCTCCGAAGACGAAAAATTCACTTGGTTGCGGCAACTGATCGAATGGCAAAGCGACCTCAAGGATGCTGAAGAA
TACATTGACAACCTCAAGGACAATCTCTTTGAAGATGATGTGTATGTCTTTACCCCCAATGGGGATGTGGTGGCCCTGGC
CAAAGGTGCTACTTCCATCGACTTCGCCTATCGCATTCACACCGAGGTGGGGAACCACATGAAAGGGGCGCGGATCAATG
GTCGTTGGTCGGTGTTAGAAAAAAAACTCCGCAATGGCGATATTGTCGAAATTATCACCCAAAAAAATGCCCACCCCAGC
CTCGATTGGCTCAATTATGTAGTTACTCCCAGTGCCAAAAATAGGATTCGCCAATGGTTTAAGCGATCGCGGCGGGATGA
AAACTTAGCACGGGGTCGCAGCTTACTCGAAAAAGAACTGGGAAAAACGGGCCTCGATTCGATCCTCAAATCCGAGGCGA
TGCAAACCGTTGCTCACAAGTGCAATTACCAAAATACCGAGGATCTGTTGGCGGCTTTGGGTTACGGCGAAGTCACCCTC
AACCAAGTGGTGAACCGCTGGCGGGATCAAGTGCGCGACCAGGAAGAAGAACATCTCCCCCAACTGGAACTAGAAGCTTC
TTTAAACACAGCGACGAAACCGGCCAAGCCCCTCCAAGATAGCCATAAATACCCCATTGCTGGCATTGAAGGGTTGGTTT
ATACCATCGCCAGTTGTTGTGCCCCCTTGCCCGGCGAACCGATTATCGGCATTGTCACCCGCAGCCATAAGGGGATTTCC
ATTCACCACCGCAGCTGCGCCAATGTGCAAAACTTCGATGGCGATCGCCTAATTCCTGTGAGTTGGAATCCCAGCATCGA
CCAAAAGCATGCTCCGGTCTACCCTGTGGATCTCCGCATTGAAGTCATTGACCGGGTGGGAGTCCTACGAGATATCCTGT
CACGCCTGAGCGATCAGCACATCAACGTGCGGAGTACCAACGTCAAAACGAGCCACAGCCAACCAGCGATTATTTCTTTG
CGGATTGAAATTCGTAATGCCCAACAACTGGCCCACAGCATTAATCAAATTAAAAACATGAGCGATACTTTGAACGTGCG
CCGCGTCACCCAAATCGAGCAGGAATAG

Upstream 100 bases:

>100_bases
CGAGGAATGCGGGTTAAGGTTGCCCCAAATATCGCCTTCTATTACAACCTTTTTAAGCTCGTCCGCTGTCGTTGTTGTTT
TTCTTCTCCCCTGTGTTTCT

Downstream 100 bases:

>100_bases
CAATCTAGGTGATCATACTGAAAATCTCTGAAAGGTCGTCAAATTAACATCAGAGGGCCGATTGCCTAGGACAGTAACCC
GTTAGAATAACCATATAGTT

Product: GTP pyrophosphokinase/ RelA/SpoT family protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MTASPVQLTAASETEKFDVPLPAWLKNYLYQPEQLVGVSEGDRQLIGEAFRFAHALHEGQTRKSGEPYIAHPIAVAGLLL
DLGGNGAMIAAGFLHDVVEDTEVSPEEIEERFGAETRKLVEGVTKLTKFTFSSKTERQAENFRRMFLSMAEDIRVIVVKL
ADRLHNMRTLEHLRPDKQERIALETREIFAPLANRLGIWRFKWELEDLCFKYLEREAYRSMQKHISEKRTEREAKLEEAI
EIIRDRLRHLGLHVWEIKGRPKHLYSIYHKMQRQHKEFDEIFDIAGIRIIVETNDECYRALAVIHDAFKPIPGRFKDYIG
LPKPNRYQSLHTTVVGLNGRPLEVQIRTMEMHHIAEYGIAAHWKYKEAGHSAASFTSEDEKFTWLRQLIEWQSDLKDAEE
YIDNLKDNLFEDDVYVFTPNGDVVALAKGATSIDFAYRIHTEVGNHMKGARINGRWSVLEKKLRNGDIVEIITQKNAHPS
LDWLNYVVTPSAKNRIRQWFKRSRRDENLARGRSLLEKELGKTGLDSILKSEAMQTVAHKCNYQNTEDLLAALGYGEVTL
NQVVNRWRDQVRDQEEEHLPQLELEASLNTATKPAKPLQDSHKYPIAGIEGLVYTIASCCAPLPGEPIIGIVTRSHKGIS
IHHRSCANVQNFDGDRLIPVSWNPSIDQKHAPVYPVDLRIEVIDRVGVLRDILSRLSDQHINVRSTNVKTSHSQPAIISL
RIEIRNAQQLAHSINQIKNMSDTLNVRRVTQIEQE

Sequences:

>Translated_755_residues
MTASPVQLTAASETEKFDVPLPAWLKNYLYQPEQLVGVSEGDRQLIGEAFRFAHALHEGQTRKSGEPYIAHPIAVAGLLL
DLGGNGAMIAAGFLHDVVEDTEVSPEEIEERFGAETRKLVEGVTKLTKFTFSSKTERQAENFRRMFLSMAEDIRVIVVKL
ADRLHNMRTLEHLRPDKQERIALETREIFAPLANRLGIWRFKWELEDLCFKYLEREAYRSMQKHISEKRTEREAKLEEAI
EIIRDRLRHLGLHVWEIKGRPKHLYSIYHKMQRQHKEFDEIFDIAGIRIIVETNDECYRALAVIHDAFKPIPGRFKDYIG
LPKPNRYQSLHTTVVGLNGRPLEVQIRTMEMHHIAEYGIAAHWKYKEAGHSAASFTSEDEKFTWLRQLIEWQSDLKDAEE
YIDNLKDNLFEDDVYVFTPNGDVVALAKGATSIDFAYRIHTEVGNHMKGARINGRWSVLEKKLRNGDIVEIITQKNAHPS
LDWLNYVVTPSAKNRIRQWFKRSRRDENLARGRSLLEKELGKTGLDSILKSEAMQTVAHKCNYQNTEDLLAALGYGEVTL
NQVVNRWRDQVRDQEEEHLPQLELEASLNTATKPAKPLQDSHKYPIAGIEGLVYTIASCCAPLPGEPIIGIVTRSHKGIS
IHHRSCANVQNFDGDRLIPVSWNPSIDQKHAPVYPVDLRIEVIDRVGVLRDILSRLSDQHINVRSTNVKTSHSQPAIISL
RIEIRNAQQLAHSINQIKNMSDTLNVRRVTQIEQE
>Mature_754_residues
TASPVQLTAASETEKFDVPLPAWLKNYLYQPEQLVGVSEGDRQLIGEAFRFAHALHEGQTRKSGEPYIAHPIAVAGLLLD
LGGNGAMIAAGFLHDVVEDTEVSPEEIEERFGAETRKLVEGVTKLTKFTFSSKTERQAENFRRMFLSMAEDIRVIVVKLA
DRLHNMRTLEHLRPDKQERIALETREIFAPLANRLGIWRFKWELEDLCFKYLEREAYRSMQKHISEKRTEREAKLEEAIE
IIRDRLRHLGLHVWEIKGRPKHLYSIYHKMQRQHKEFDEIFDIAGIRIIVETNDECYRALAVIHDAFKPIPGRFKDYIGL
PKPNRYQSLHTTVVGLNGRPLEVQIRTMEMHHIAEYGIAAHWKYKEAGHSAASFTSEDEKFTWLRQLIEWQSDLKDAEEY
IDNLKDNLFEDDVYVFTPNGDVVALAKGATSIDFAYRIHTEVGNHMKGARINGRWSVLEKKLRNGDIVEIITQKNAHPSL
DWLNYVVTPSAKNRIRQWFKRSRRDENLARGRSLLEKELGKTGLDSILKSEAMQTVAHKCNYQNTEDLLAALGYGEVTLN
QVVNRWRDQVRDQEEEHLPQLELEASLNTATKPAKPLQDSHKYPIAGIEGLVYTIASCCAPLPGEPIIGIVTRSHKGISI
HHRSCANVQNFDGDRLIPVSWNPSIDQKHAPVYPVDLRIEVIDRVGVLRDILSRLSDQHINVRSTNVKTSHSQPAIISLR
IEIRNAQQLAHSINQIKNMSDTLNVRRVTQIEQE

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=135, Percent_Identity=39.2592592592593, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1790082, Length=704, Percent_Identity=39.7727272727273, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1789147, Length=712, Percent_Identity=36.2359550561798, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI25143118, Length=138, Percent_Identity=35.5072463768116, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 86629; Mature: 86498

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPVQLTAASETEKFDVPLPAWLKNYLYQPEQLVGVSEGDRQLIGEAFRFAHALHEGQ
CCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCC
TRKSGEPYIAHPIAVAGLLLDLGGNGAMIAAGFLHDVVEDTEVSPEEIEERFGAETRKLV
CCCCCCCEEEHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHH
EGVTKLTKFTFSSKTERQAENFRRMFLSMAEDIRVIVVKLADRLHNMRTLEHLRPDKQER
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IALETREIFAPLANRLGIWRFKWELEDLCFKYLEREAYRSMQKHISEKRTEREAKLEEAI
HHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIIRDRLRHLGLHVWEIKGRPKHLYSIYHKMQRQHKEFDEIFDIAGIRIIVETNDECYRA
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHH
LAVIHDAFKPIPGRFKDYIGLPKPNRYQSLHTTVVGLNGRPLEVQIRTMEMHHIAEYGIA
HHHHHHHHCCCCCHHHHHCCCCCCCCHHHHEEEEEECCCCCEEEEEEEHHHHHHHHHCCC
AHWKYKEAGHSAASFTSEDEKFTWLRQLIEWQSDLKDAEEYIDNLKDNLFEDDVYVFTPN
EEEEHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GDVVALAKGATSIDFAYRIHTEVGNHMKGARINGRWSVLEKKLRNGDIVEIITQKNAHPS
CCEEEEECCCCEEEEEEEEEHHHCCCCCCCEECCHHHHHHHHHCCCCEEEEEECCCCCCC
LDWLNYVVTPSAKNRIRQWFKRSRRDENLARGRSLLEKELGKTGLDSILKSEAMQTVAHK
HHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CNYQNTEDLLAALGYGEVTLNQVVNRWRDQVRDQEEEHLPQLELEASLNTATKPAKPLQD
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCEEEHHCCCCCCCCCCCCCC
SHKYPIAGIEGLVYTIASCCAPLPGEPIIGIVTRSHKGISIHHRSCANVQNFDGDRLIPV
CCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEE
SWNPSIDQKHAPVYPVDLRIEVIDRVGVLRDILSRLSDQHINVRSTNVKTSHSQPAIISL
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEE
RIEIRNAQQLAHSINQIKNMSDTLNVRRVTQIEQE
EEEECHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TASPVQLTAASETEKFDVPLPAWLKNYLYQPEQLVGVSEGDRQLIGEAFRFAHALHEGQ
CCCCCEEEECCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCC
TRKSGEPYIAHPIAVAGLLLDLGGNGAMIAAGFLHDVVEDTEVSPEEIEERFGAETRKLV
CCCCCCCEEEHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHH
EGVTKLTKFTFSSKTERQAENFRRMFLSMAEDIRVIVVKLADRLHNMRTLEHLRPDKQER
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IALETREIFAPLANRLGIWRFKWELEDLCFKYLEREAYRSMQKHISEKRTEREAKLEEAI
HHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIIRDRLRHLGLHVWEIKGRPKHLYSIYHKMQRQHKEFDEIFDIAGIRIIVETNDECYRA
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHH
LAVIHDAFKPIPGRFKDYIGLPKPNRYQSLHTTVVGLNGRPLEVQIRTMEMHHIAEYGIA
HHHHHHHHCCCCCHHHHHCCCCCCCCHHHHEEEEEECCCCCEEEEEEEHHHHHHHHHCCC
AHWKYKEAGHSAASFTSEDEKFTWLRQLIEWQSDLKDAEEYIDNLKDNLFEDDVYVFTPN
EEEEHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GDVVALAKGATSIDFAYRIHTEVGNHMKGARINGRWSVLEKKLRNGDIVEIITQKNAHPS
CCEEEEECCCCEEEEEEEEEHHHCCCCCCCEECCHHHHHHHHHCCCCEEEEEECCCCCCC
LDWLNYVVTPSAKNRIRQWFKRSRRDENLARGRSLLEKELGKTGLDSILKSEAMQTVAHK
HHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CNYQNTEDLLAALGYGEVTLNQVVNRWRDQVRDQEEEHLPQLELEASLNTATKPAKPLQD
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCEEEHHCCCCCCCCCCCCCC
SHKYPIAGIEGLVYTIASCCAPLPGEPIIGIVTRSHKGISIHHRSCANVQNFDGDRLIPV
CCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEE
SWNPSIDQKHAPVYPVDLRIEVIDRVGVLRDILSRLSDQHINVRSTNVKTSHSQPAIISL
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEE
RIEIRNAQQLAHSINQIKNMSDTLNVRRVTQIEQE
EEEECHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]