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The map label for this gene is ligA

Identifier: 170079241

GI number: 170079241

Start: 2781975

End: 2784011

Strand: Direct

Name: ligA

Synonym: SYNPCC7002_A2647

Alternate gene names: 170079241

Gene position: 2781975-2784011 (Clockwise)

Preceding gene: 170079240

Following gene: 170079242

Centisome position: 92.48

GC content: 50.71

Gene sequence:

>2037_bases
ATGTCCGACGCCCCCCCAACCCGCCTGGCCCAACTGCGATCGCAACTGCAAAAAGCGGCCTATGCCTACTATGTCCTCGA
TGCGCCGTTCATGGAGGATGCTGTCTATGACCAGCTCTATCGGGAACTGGTGGCCCTGGAAACGGCACATCCCCAACTCA
TCACCCCCGACAGTCCTACCCAACGGATTGGCGACCAGCCCGCCACCCAATTCACCAGCGTCAAACATAATATCCCCCTC
TACAGTCTGGAAAATGCCTTTGACTTTGGGGAACTGACCCAGTGGGAACAACGGTGGCAGCGGGCGTTGGCCGGAGAAAT
CCCCAGCGATTCCGGTTATGTGTGCGAACTTAAAATCGATGGCTCGGCGATCGCCTTAACTTACGAAAACGGGATTTTAG
TGCGCGGAGCCACCAGGGGCGACGGTGTGACAGGAGAAGAAATCACCCAAAATCTGCGTACTATTCGTACCATTCCTTTA
CGTTTAAACCTCGATAATCCGCCTCCTTTAGTGGAGGTGCGGGGGGAAGCTTTTTTACCCCTAGAGACCTTTGAAAAGAT
TAACCAAGAGCGGGAAAAAGCGGGCGAAAATCTATTTGCTAATCCGCGAAATGCCGCCGCTGGTACCCTACGACAACTTG
ATTCCCGAATTGTTGCGGCTCGCAAGTTAGACTTTTTTGCCTACACCCTCCACCTCCCAGAATCGCACAATTCCCCTCAG
TTAAATAGCCAATGGGAGGCCCTCGAATTTCTGAAAAAAATGGGGTTTCGGGTCAATCCAAACCGTGAGCTTTGCCCTAA
TTTAACTGCCGTAAAAAATTATTTTGAAAAATGGGATGAAGGCCGCAAAAAACTCCCTTACCTGACCGATGGGGTTGTCG
TTAAACTCAATGACTTTTCTCTCCAGGCTGAGTTGGGCTTCACCCAAAAATTTCCCCGCTGGGCGATCGCCTTAAAATAT
GCCGCCGAGGAAGCCCCAACCACTGTCAAAGACATCATCGTCAACGTGGGCAGAACGGGGGCTGTCACCCCCATGGCCGT
CATGGAACCAGTGCAATTGGCCGGAACAACGGTACAACGGGCCACCCTCCACAACGAAGACCGCATTAAAGAATTAGATC
TTAGAATCGGTGATACAGTCGTCATTCGCAAAGCAGGGGAAATTATCCCGGAAGTTTTGCGAGTTTTAGTTGATTTACGC
CCAGAAGCAACTATTCCTTACGAATTTCCAAGCCATTGTCCCGAATGCAACTCAGCCCTGGTTCGTCCCCCTGGGGAAGC
CGTCGCCCGCTGTGTGAATAGTTCTTGTCCGGCGATTTTGCGGGGTTCCCTCGTCCATTGGGCTGGCCGCGACGCCCTCG
ATATCCAGGGTTTAGGCGAAAAAAATGTGATTCTTTTGATTGAGCATGGTCTGGTGCAATCCATCGCCGACATTTATGAC
CTAACCAGCGAACAGTTGCAGTCTCTCCCCCGTATGGGACAAAAGTCAGCGGCGAATTTAATCCAGGCGATCGCCCACTC
AAAAAATCAAGACTATGCCCGTGTTTTGTATGGTCTGGGGATTCGCTTTGTGGGGAAAGTCAATGCAGAAATTTTGGCGC
AAAACTTCCCAACTTTAGAACAACTCCAAAAAGCCAGCTTCGCTCAATTACAGGGGGTTTATGGCATTGGCGAAGAAATT
GCCCAATCCGTCGTCGATTGGTTCCGGGTAGATGCGAATCAACAGTTGATCGAAAAGCTACAACAAGCGGGCTTGACCTT
TGCCCAAACCCAGACGGCCCAAACCCAGACGGCCCAAACCCAAGCCAATCATCAGATTTCCGGTAAAACCTTTGTGATCA
CAGGGACATTACCGACCCTCAAACGCACTGAAGCTGCCGAAAAAATCAAAGCAGCCGGCGGCAAAGTCACGAGTTCTGTC
AGCAAAAATACTGACTATCTTGTGGCGGGCGACAATGCCGGATCTAAACTAGACAAAGCCCGACAATTGGGAATTCAAGA
ACTGAATGAAAACCAGCTTTTAGAGTTGTTGGTATAG

Upstream 100 bases:

>100_bases
AGCCGTCCCCACCGAAACCGACCCAGAAGCAGAGGCAACGGAATAATTTCCCTGGGGCGATCGCCTATAATGGCGGCTTG
AAAATCTTTCCCCCAAACCC

Downstream 100 bases:

>100_bases
AAAAAATCTTTTGAATATGACGCAATCTCAACCGCCAATTGCCGCCCTGGCGCTCCGTTAGAGAATCGATGGCAAGTGAC
TTGGGGTTCACTTTGAGTAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSDAPPTRLAQLRSQLQKAAYAYYVLDAPFMEDAVYDQLYRELVALETAHPQLITPDSPTQRIGDQPATQFTSVKHNIPL
YSLENAFDFGELTQWEQRWQRALAGEIPSDSGYVCELKIDGSAIALTYENGILVRGATRGDGVTGEEITQNLRTIRTIPL
RLNLDNPPPLVEVRGEAFLPLETFEKINQEREKAGENLFANPRNAAAGTLRQLDSRIVAARKLDFFAYTLHLPESHNSPQ
LNSQWEALEFLKKMGFRVNPNRELCPNLTAVKNYFEKWDEGRKKLPYLTDGVVVKLNDFSLQAELGFTQKFPRWAIALKY
AAEEAPTTVKDIIVNVGRTGAVTPMAVMEPVQLAGTTVQRATLHNEDRIKELDLRIGDTVVIRKAGEIIPEVLRVLVDLR
PEATIPYEFPSHCPECNSALVRPPGEAVARCVNSSCPAILRGSLVHWAGRDALDIQGLGEKNVILLIEHGLVQSIADIYD
LTSEQLQSLPRMGQKSAANLIQAIAHSKNQDYARVLYGLGIRFVGKVNAEILAQNFPTLEQLQKASFAQLQGVYGIGEEI
AQSVVDWFRVDANQQLIEKLQQAGLTFAQTQTAQTQTAQTQANHQISGKTFVITGTLPTLKRTEAAEKIKAAGGKVTSSV
SKNTDYLVAGDNAGSKLDKARQLGIQELNENQLLELLV

Sequences:

>Translated_678_residues
MSDAPPTRLAQLRSQLQKAAYAYYVLDAPFMEDAVYDQLYRELVALETAHPQLITPDSPTQRIGDQPATQFTSVKHNIPL
YSLENAFDFGELTQWEQRWQRALAGEIPSDSGYVCELKIDGSAIALTYENGILVRGATRGDGVTGEEITQNLRTIRTIPL
RLNLDNPPPLVEVRGEAFLPLETFEKINQEREKAGENLFANPRNAAAGTLRQLDSRIVAARKLDFFAYTLHLPESHNSPQ
LNSQWEALEFLKKMGFRVNPNRELCPNLTAVKNYFEKWDEGRKKLPYLTDGVVVKLNDFSLQAELGFTQKFPRWAIALKY
AAEEAPTTVKDIIVNVGRTGAVTPMAVMEPVQLAGTTVQRATLHNEDRIKELDLRIGDTVVIRKAGEIIPEVLRVLVDLR
PEATIPYEFPSHCPECNSALVRPPGEAVARCVNSSCPAILRGSLVHWAGRDALDIQGLGEKNVILLIEHGLVQSIADIYD
LTSEQLQSLPRMGQKSAANLIQAIAHSKNQDYARVLYGLGIRFVGKVNAEILAQNFPTLEQLQKASFAQLQGVYGIGEEI
AQSVVDWFRVDANQQLIEKLQQAGLTFAQTQTAQTQTAQTQANHQISGKTFVITGTLPTLKRTEAAEKIKAAGGKVTSSV
SKNTDYLVAGDNAGSKLDKARQLGIQELNENQLLELLV
>Mature_677_residues
SDAPPTRLAQLRSQLQKAAYAYYVLDAPFMEDAVYDQLYRELVALETAHPQLITPDSPTQRIGDQPATQFTSVKHNIPLY
SLENAFDFGELTQWEQRWQRALAGEIPSDSGYVCELKIDGSAIALTYENGILVRGATRGDGVTGEEITQNLRTIRTIPLR
LNLDNPPPLVEVRGEAFLPLETFEKINQEREKAGENLFANPRNAAAGTLRQLDSRIVAARKLDFFAYTLHLPESHNSPQL
NSQWEALEFLKKMGFRVNPNRELCPNLTAVKNYFEKWDEGRKKLPYLTDGVVVKLNDFSLQAELGFTQKFPRWAIALKYA
AEEAPTTVKDIIVNVGRTGAVTPMAVMEPVQLAGTTVQRATLHNEDRIKELDLRIGDTVVIRKAGEIIPEVLRVLVDLRP
EATIPYEFPSHCPECNSALVRPPGEAVARCVNSSCPAILRGSLVHWAGRDALDIQGLGEKNVILLIEHGLVQSIADIYDL
TSEQLQSLPRMGQKSAANLIQAIAHSKNQDYARVLYGLGIRFVGKVNAEILAQNFPTLEQLQKASFAQLQGVYGIGEEIA
QSVVDWFRVDANQQLIEKLQQAGLTFAQTQTAQTQTAQTQANHQISGKTFVITGTLPTLKRTEAAEKIKAAGGKVTSSVS
KNTDYLVAGDNAGSKLDKARQLGIQELNENQLLELLV

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=45.3055141579732, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI87082305, Length=577, Percent_Identity=24.4367417677643, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SYNP2 (B1XLV0)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001735879.1
- ProteinModelPortal:   B1XLV0
- GeneID:   6056311
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A2647
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75070; Mature: 74939

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 118-118 BINDING 116-116 BINDING 139-139 BINDING 176-176 BINDING 295-295 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAPPTRLAQLRSQLQKAAYAYYVLDAPFMEDAVYDQLYRELVALETAHPQLITPDSPT
CCCCCHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCH
QRIGDQPATQFTSVKHNIPLYSLENAFDFGELTQWEQRWQRALAGEIPSDSGYVCELKID
HHCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
GSAIALTYENGILVRGATRGDGVTGEEITQNLRTIRTIPLRLNLDNPPPLVEVRGEAFLP
CCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCEEEECCCEECC
LETFEKINQEREKAGENLFANPRNAAAGTLRQLDSRIVAARKLDFFAYTLHLPESHNSPQ
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCC
LNSQWEALEFLKKMGFRVNPNRELCPNLTAVKNYFEKWDEGRKKLPYLTDGVVVKLNDFS
CCCHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCE
LQAELGFTQKFPRWAIALKYAAEEAPTTVKDIIVNVGRTGAVTPMAVMEPVQLAGTTVQR
EEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHCCHHHHCCHHHH
ATLHNEDRIKELDLRIGDTVVIRKAGEIIPEVLRVLVDLRPEATIPYEFPSHCPECNSAL
HHCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
VRPPGEAVARCVNSSCPAILRGSLVHWAGRDALDIQGLGEKNVILLIEHGLVQSIADIYD
CCCCHHHHHHHHCCCCCHHHHCCHHEECCCCCEEECCCCCCCEEEEECCHHHHHHHHHHH
LTSEQLQSLPRMGQKSAANLIQAIAHSKNQDYARVLYGLGIRFVGKVNAEILAQNFPTLE
CCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEEECCCHHHHHCCCCHHH
QLQKASFAQLQGVYGIGEEIAQSVVDWFRVDANQQLIEKLQQAGLTFAQTQTAQTQTAQT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCHHHHHHHHH
QANHQISGKTFVITGTLPTLKRTEAAEKIKAAGGKVTSSVSKNTDYLVAGDNAGSKLDKA
HCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCHHHHHH
RQLGIQELNENQLLELLV
HHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
SDAPPTRLAQLRSQLQKAAYAYYVLDAPFMEDAVYDQLYRELVALETAHPQLITPDSPT
CCCCHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCH
QRIGDQPATQFTSVKHNIPLYSLENAFDFGELTQWEQRWQRALAGEIPSDSGYVCELKID
HHCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
GSAIALTYENGILVRGATRGDGVTGEEITQNLRTIRTIPLRLNLDNPPPLVEVRGEAFLP
CCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCEEEECCCEECC
LETFEKINQEREKAGENLFANPRNAAAGTLRQLDSRIVAARKLDFFAYTLHLPESHNSPQ
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCC
LNSQWEALEFLKKMGFRVNPNRELCPNLTAVKNYFEKWDEGRKKLPYLTDGVVVKLNDFS
CCCHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCE
LQAELGFTQKFPRWAIALKYAAEEAPTTVKDIIVNVGRTGAVTPMAVMEPVQLAGTTVQR
EEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHCCHHHHCCHHHH
ATLHNEDRIKELDLRIGDTVVIRKAGEIIPEVLRVLVDLRPEATIPYEFPSHCPECNSAL
HHCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
VRPPGEAVARCVNSSCPAILRGSLVHWAGRDALDIQGLGEKNVILLIEHGLVQSIADIYD
CCCCHHHHHHHHCCCCCHHHHCCHHEECCCCCEEECCCCCCCEEEEECCHHHHHHHHHHH
LTSEQLQSLPRMGQKSAANLIQAIAHSKNQDYARVLYGLGIRFVGKVNAEILAQNFPTLE
CCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEEECCCHHHHHCCCCHHH
QLQKASFAQLQGVYGIGEEIAQSVVDWFRVDANQQLIEKLQQAGLTFAQTQTAQTQTAQT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCHHHHHHHHH
QANHQISGKTFVITGTLPTLKRTEAAEKIKAAGGKVTSSVSKNTDYLVAGDNAGSKLDKA
HCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCHHHHHH
RQLGIQELNENQLLELLV
HHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA