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Identifier: 170078943

GI number: 170078943

Start: 2427025

End: 2427588

Strand: Reverse

Name: def

Synonym: SYNPCC7002_A2348

Alternate gene names: 170078943

Gene position: 2427588-2427025 (Counterclockwise)

Preceding gene: 170078946

Following gene: 170078942

Centisome position: 80.7

GC content: 46.28

Gene sequence:

>564_bases
ATGACTGTTGGTATATCTGTCGAAAAAGGAAAGCAAGATACTCCTCCCTTAGAACTCCATTACCTCGGCGACAAAGTGCT
ACGCCAAAAAGCAAAACGCATCGCCAAAGTAGATGACGAAATCCGCACCCTGGCGAAGGAAATGCTGCAAACCATGTATT
CTTCCCAGGGAATCGGTCTGGCTGCGCCCCAAGTTGGTGTTAATAAGCGTTTAATCGTCATTGATACCGATCCAGAAAAT
CCTGCCAATGCACCACTGGTTTTAATCAACCCTGAAATCAAAAAATTTGGACAACAGCTTTGTCCCTTTGAGGAAGGCTG
TCTCAGCATTCCTGGGGTTCACTTAGATGTCATTCGCCCCGATGAAATTGAAGTAAGTTACCGAGATGAACAAGGTAAAC
CCAAACGTATCAAAGCTTCTGGCCTGTTGTCCCGGGTGATTCAGCATGAAATTGACCATCTCGATGGGGTGATGTTCGTC
GATCGCGTCGAAAATGAAATTGCCCTCAGTAGCCAATTAAAACAACGGGGATTTGCCCTGAAATCGGTACAGCGCATCGC
CTAA

Upstream 100 bases:

>100_bases
CCGCAATCCGCCAATTACAGGAAGCCATAGGAATCCTTGTTAAAATGGAAGCACATGATATCAAGAAAAATTGTCGTTAC
CCTAAAACTTTAGAAAATTT

Downstream 100 bases:

>100_bases
ATTTTTTTTGACCTTTTCCACGTTTCAACATGACGGCAACGTAGTACTCTACGACGTTTGTGTGAGGCGATCGCCGTCCA
GTGAAATAGACGTTCTGCCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTVGISVEKGKQDTPPLELHYLGDKVLRQKAKRIAKVDDEIRTLAKEMLQTMYSSQGIGLAAPQVGVNKRLIVIDTDPEN
PANAPLVLINPEIKKFGQQLCPFEEGCLSIPGVHLDVIRPDEIEVSYRDEQGKPKRIKASGLLSRVIQHEIDHLDGVMFV
DRVENEIALSSQLKQRGFALKSVQRIA

Sequences:

>Translated_187_residues
MTVGISVEKGKQDTPPLELHYLGDKVLRQKAKRIAKVDDEIRTLAKEMLQTMYSSQGIGLAAPQVGVNKRLIVIDTDPEN
PANAPLVLINPEIKKFGQQLCPFEEGCLSIPGVHLDVIRPDEIEVSYRDEQGKPKRIKASGLLSRVIQHEIDHLDGVMFV
DRVENEIALSSQLKQRGFALKSVQRIA
>Mature_186_residues
TVGISVEKGKQDTPPLELHYLGDKVLRQKAKRIAKVDDEIRTLAKEMLQTMYSSQGIGLAAPQVGVNKRLIVIDTDPENP
ANAPLVLINPEIKKFGQQLCPFEEGCLSIPGVHLDVIRPDEIEVSYRDEQGKPKRIKASGLLSRVIQHEIDHLDGVMFVD
RVENEIALSSQLKQRGFALKSVQRIA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=173, Percent_Identity=30.0578034682081, Blast_Score=84, Evalue=8e-17,
Organism=Escherichia coli, GI1789682, Length=146, Percent_Identity=44.5205479452055, Blast_Score=110, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24645728, Length=170, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24645726, Length=173, Percent_Identity=29.4797687861272, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_SYNP2 (B1XJP0)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001735581.1
- ProteinModelPortal:   B1XJP0
- SMR:   B1XJP0
- GeneID:   6056809
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A2348
- HOGENOM:   HBG665227
- OMA:   DHVENEL
- ProtClustDB:   PRK00150
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20828; Mature: 20697

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: ACT_SITE 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGISVEKGKQDTPPLELHYLGDKVLRQKAKRIAKVDDEIRTLAKEMLQTMYSSQGIGL
CEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
AAPQVGVNKRLIVIDTDPENPANAPLVLINPEIKKFGQQLCPFEEGCLSIPGVHLDVIRP
CCCCCCCCCEEEEEECCCCCCCCCCEEEECHHHHHHHHHCCCHHHCCCCCCCCEEEEECC
DEIEVSYRDEQGKPKRIKASGLLSRVIQHEIDHLDGVMFVDRVENEIALSSQLKQRGFAL
CCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHH
KSVQRIA
HHHHHCC
>Mature Secondary Structure 
TVGISVEKGKQDTPPLELHYLGDKVLRQKAKRIAKVDDEIRTLAKEMLQTMYSSQGIGL
EECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
AAPQVGVNKRLIVIDTDPENPANAPLVLINPEIKKFGQQLCPFEEGCLSIPGVHLDVIRP
CCCCCCCCCEEEEEECCCCCCCCCCEEEECHHHHHHHHHCCCHHHCCCCCCCCEEEEECC
DEIEVSYRDEQGKPKRIKASGLLSRVIQHEIDHLDGVMFVDRVENEIALSSQLKQRGFAL
CCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHH
KSVQRIA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA