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The map label for this gene is braE [H]

Identifier: 170078692

GI number: 170078692

Start: 2167705

End: 2168805

Strand: Reverse

Name: braE [H]

Synonym: SYNPCC7002_A2091

Alternate gene names: 170078692

Gene position: 2168805-2167705 (Counterclockwise)

Preceding gene: 170078693

Following gene: 170078691

Centisome position: 72.1

GC content: 50.95

Gene sequence:

>1101_bases
ATGGTCGGCTATCTCGTCTATCTCACCATCTCAGCGGCAACCTACGGCATTTTTTGTCTGGGCCTCAATTTGCAATGGGG
CTTTACGGGCCTGATTAACTTTGGTCATGTGGCTTTTATGACCGTCGGTGCCTATGCCACAGTTTTACTTTCCAGCCAAG
GCGTGCCCTTACTGCTTTCTCTCGCCATTGGGGCTGGGGTCGCGGCTTTACTGGGGCTGCTCATTGGCCTCTCAACCCTG
CGGCTGCGGGAAGATTATTTGGCAATTGTGACCATTGGGGTGTCTGAATTGGTGCGGTTGGTGGCCCTCAACGAAGAATG
GTTAACCAGGGGAGCCTTTGGGGTGCAGGGTTATCCGTTGCCCCTGGGAAGTTTTCGACCGAATCAGGTGGGTAAGATTT
CTTTGATTTTTTGGTTGACGATCATTGCAATTTTTGCGGCATGGCAACTATGGCGGGGGTTGCGCTGGCGCTGGAAAAAA
CTCAAAAAACAGGAAAAATCTGGTTTAGGGGTGATTGTCTGGGGCATCCTTGGGGGCGGCATCATCACCAGTTTGTTTAT
CAACGGCTGCATTGCCCTCCAAAATTACAACTACAAAGCAGGGCTGATGTTGCTGTCGGTGGTCGCCTTGACTTTGGTTT
ATGCCGGTCTAGAGTATTTAGTGCAATCCCCCTGGGGTCGGGTGCTAAAGGCGATTCGCGAAGATGAGGAAATCCCCCGT
GCCCTCGGTAAAAATGTTTTTTGGTATAAGTTGCAGGCCTTTATGCTCGGTGGGGCGATCGCCGGTGGAGCGGGTTCTTT
TTATGCATGGCAGTTGACTAATGTTTATCCGGCTAATTTTGATCCGTTAGTCACCTTCAACGCTTGGACGATTGTGGTGT
TGGGCGGCGCTGGCACGAATCCCGGTACTTTAATTGGTGCGCTCATTTTCTTTGCCTACGACTCCCTGACGCGGTTTGTG
TTGCCCCAGTTAAATTTGGTGGACGATGCCCGCCTCGGCGCATTACGGATAATGGTGATTGGTCTAATTTTGATGGTGTT
GATGGTATGGCGGCCCCAGGGGATTTTGGGGAATAAAGATGAGTTGACCCTTGGTAAATAA

Upstream 100 bases:

>100_bases
GGGGGGAATGGATGCTATATGATGGGGCAGCTTGAGGCTTTACTTCGTCTTTTTAAAACAAGCTTTTTAATCTCCATCCC
TATAAAAATCACAAATCACT

Downstream 100 bases:

>100_bases
TTGGTAAAATTTCTGGCATTACCGTTATTACTGCTGAGATAAACATAAAGTCCTGGCATTTCCTACGTTAATCTATGGGA
AATGCTTTTTTGTCGCCTAT

Product: high-affinity branched-chain amino acid transport protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MVGYLVYLTISAATYGIFCLGLNLQWGFTGLINFGHVAFMTVGAYATVLLSSQGVPLLLSLAIGAGVAALLGLLIGLSTL
RLREDYLAIVTIGVSELVRLVALNEEWLTRGAFGVQGYPLPLGSFRPNQVGKISLIFWLTIIAIFAAWQLWRGLRWRWKK
LKKQEKSGLGVIVWGILGGGIITSLFINGCIALQNYNYKAGLMLLSVVALTLVYAGLEYLVQSPWGRVLKAIREDEEIPR
ALGKNVFWYKLQAFMLGGAIAGGAGSFYAWQLTNVYPANFDPLVTFNAWTIVVLGGAGTNPGTLIGALIFFAYDSLTRFV
LPQLNLVDDARLGALRIMVIGLILMVLMVWRPQGILGNKDELTLGK

Sequences:

>Translated_366_residues
MVGYLVYLTISAATYGIFCLGLNLQWGFTGLINFGHVAFMTVGAYATVLLSSQGVPLLLSLAIGAGVAALLGLLIGLSTL
RLREDYLAIVTIGVSELVRLVALNEEWLTRGAFGVQGYPLPLGSFRPNQVGKISLIFWLTIIAIFAAWQLWRGLRWRWKK
LKKQEKSGLGVIVWGILGGGIITSLFINGCIALQNYNYKAGLMLLSVVALTLVYAGLEYLVQSPWGRVLKAIREDEEIPR
ALGKNVFWYKLQAFMLGGAIAGGAGSFYAWQLTNVYPANFDPLVTFNAWTIVVLGGAGTNPGTLIGALIFFAYDSLTRFV
LPQLNLVDDARLGALRIMVIGLILMVLMVWRPQGILGNKDELTLGK
>Mature_366_residues
MVGYLVYLTISAATYGIFCLGLNLQWGFTGLINFGHVAFMTVGAYATVLLSSQGVPLLLSLAIGAGVAALLGLLIGLSTL
RLREDYLAIVTIGVSELVRLVALNEEWLTRGAFGVQGYPLPLGSFRPNQVGKISLIFWLTIIAIFAAWQLWRGLRWRWKK
LKKQEKSGLGVIVWGILGGGIITSLFINGCIALQNYNYKAGLMLLSVVALTLVYAGLEYLVQSPWGRVLKAIREDEEIPR
ALGKNVFWYKLQAFMLGGAIAGGAGSFYAWQLTNVYPANFDPLVTFNAWTIVVLGGAGTNPGTLIGALIFFAYDSLTRFV
LPQLNLVDDARLGALRIMVIGLILMVLMVWRPQGILGNKDELTLGK

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=118, Percent_Identity=41.5254237288136, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39892; Mature: 39892

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGYLVYLTISAATYGIFCLGLNLQWGFTGLINFGHVAFMTVGAYATVLLSSQGVPLLLS
CCEEEEEEEEHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LAIGAGVAALLGLLIGLSTLRLREDYLAIVTIGVSELVRLVALNEEWLTRGAFGVQGYPL
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHCCHHHHHCCCCCCCCCCC
PLGSFRPNQVGKISLIFWLTIIAIFAAWQLWRGLRWRWKKLKKQEKSGLGVIVWGILGGG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHH
IITSLFINGCIALQNYNYKAGLMLLSVVALTLVYAGLEYLVQSPWGRVLKAIREDEEIPR
HHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALGKNVFWYKLQAFMLGGAIAGGAGSFYAWQLTNVYPANFDPLVTFNAWTIVVLGGAGTN
HHCCCCHHHHHHHHHHCCCEECCCCCEEEEEEEEECCCCCCCEEEECCEEEEEECCCCCC
PGTLIGALIFFAYDSLTRFVLPQLNLVDDARLGALRIMVIGLILMVLMVWRPQGILGNKD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ELTLGK
CCCCCC
>Mature Secondary Structure
MVGYLVYLTISAATYGIFCLGLNLQWGFTGLINFGHVAFMTVGAYATVLLSSQGVPLLLS
CCEEEEEEEEHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LAIGAGVAALLGLLIGLSTLRLREDYLAIVTIGVSELVRLVALNEEWLTRGAFGVQGYPL
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHCCHHHHHCCCCCCCCCCC
PLGSFRPNQVGKISLIFWLTIIAIFAAWQLWRGLRWRWKKLKKQEKSGLGVIVWGILGGG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHH
IITSLFINGCIALQNYNYKAGLMLLSVVALTLVYAGLEYLVQSPWGRVLKAIREDEEIPR
HHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALGKNVFWYKLQAFMLGGAIAGGAGSFYAWQLTNVYPANFDPLVTFNAWTIVVLGGAGTN
HHCCCCHHHHHHHHHHCCCEECCCCCEEEEEEEEECCCCCCCEEEECCEEEEEECCCCCC
PGTLIGALIFFAYDSLTRFVLPQLNLVDDARLGALRIMVIGLILMVLMVWRPQGILGNKD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ELTLGK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]