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The map label for this gene is tuf

Identifier: 170078665

GI number: 170078665

Start: 2144547

End: 2145776

Strand: Reverse

Name: tuf

Synonym: SYNPCC7002_A2061

Alternate gene names: 170078665

Gene position: 2145776-2144547 (Counterclockwise)

Preceding gene: 170078666

Following gene: 170078664

Centisome position: 71.33

GC content: 51.22

Gene sequence:

>1230_bases
ATGGCACGCGCTAAGTTTGAAAGAAACAAAGACCACGCCAATATTGGTACTGTTGGCCACGTTGACCACGGTAAAACAAC
GCTAACCGCAGCGATCACTATGGCCTTAGCGGCTCAAGGTGGCGGTAAAGCGAAGAGCTATGAAGATATCGACGCGGCTC
CTGAAGAAAAAGCGCGTGGTATCACCATCAACACTGCCCACGTAGAATACGAGACAGAAAATCGTCACTATGCTCACGTT
GACTGCCCTGGTCACGCTGACTATGTGAAAAACATGATCACTGGTGCAGCACAGATGGATGGCGGTATCCTTGTGGTTTC
TGCAGCCGATGGCCCGATGCCCCAGACCCGTGAACACATCCTCTTGGCCAAGCAGGTTGGTGTACCTAGCCTTGTTGTTT
TCTTGAACAAAGAAGACCAAGTTGATGACGAAGAACTCCTTGAGTTGGTTGAACTTGAAGTTCGTGAATTGCTCAGTGAA
TATGACTTCCCTGGCGATGATATTCCGATCACCACTGGTTCTGCGCTCAAGGCAGTAGAAGCCCTCGTTGCTAACCCCAA
CATCAAGCGTGGCGAAGACAAGTGGGTTGACAAAATTTTGGCCCTGATGGACAGCGTTGACGAATATATGCCCCTGCCTG
AGCGGGATGTTGACAAGCCTTTCTTGATGGCTGTAGAAGACGTCTTCTCCATTACTGGTCGTGGTACCGTTGCCACCGGT
CGGATCGAGCGTGGTCGCGTTAAAGTTGGCGAAACCATCGAAATCGTTGGGATTAGAGACACCCGCAGCACCACCGTTAC
TGGTGTTGAAATGTTCCAGAAGACCCTCGATGAAGGGATGGCTGGGGACAACGTTGGTGTACTCCTCCGTGGTGTACAAA
AGGATGATATCGAGCGCGGCATGGTCCTTGCTAAGCCCGGTTCTATCACCCCCCACACCAACTTCGAAGCTGAGGTTTAT
GTTTTGACTAAAGAAGAAGGTGGTCGTCACACGCCTTTCTTCCCCAACTACCGTCCTCAGTTCTACGTCCGGACAACTGA
CGTAACTGGTACCATCTCTGCTTTCACCGCCGATGATGGCTCCAGCGCTGAAATGGTTATGCCTGGCGATCGCATCAAGA
TGACCGTAGAACTCATCAACCCCATTGCCATTGAGCAAGGGATGCGTTTTGCGATCCGTGAAGGTGGCCGCACTATCGGT
GCTGGTACCGTTTCTAAGATCCTGAAGTAA

Upstream 100 bases:

>100_bases
TTCAGCAACTACGATGAGGTGCCTCGCCATGTGGCTGAAGCCATCATCGCAAAAAACAAAGGGAACGCAAACTTTTAACG
ATTAAGGAATAACGAAACTC

Downstream 100 bases:

>100_bases
GCCTAACGGCTGAATTCACTGGTGGGGAGCGGGTTTAAAGCATACGCCTACTCCCCATTCAGGCTCAAAACCTAGCTTGG
TAAAATTACACAGCCGCTTT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MARAKFERNKDHANIGTVGHVDHGKTTLTAAITMALAAQGGGKAKSYEDIDAAPEEKARGITINTAHVEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLAKQVGVPSLVVFLNKEDQVDDEELLELVELEVRELLSE
YDFPGDDIPITTGSALKAVEALVANPNIKRGEDKWVDKILALMDSVDEYMPLPERDVDKPFLMAVEDVFSITGRGTVATG
RIERGRVKVGETIEIVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGVLLRGVQKDDIERGMVLAKPGSITPHTNFEAEVY
VLTKEEGGRHTPFFPNYRPQFYVRTTDVTGTISAFTADDGSSAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTIG
AGTVSKILK

Sequences:

>Translated_409_residues
MARAKFERNKDHANIGTVGHVDHGKTTLTAAITMALAAQGGGKAKSYEDIDAAPEEKARGITINTAHVEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLAKQVGVPSLVVFLNKEDQVDDEELLELVELEVRELLSE
YDFPGDDIPITTGSALKAVEALVANPNIKRGEDKWVDKILALMDSVDEYMPLPERDVDKPFLMAVEDVFSITGRGTVATG
RIERGRVKVGETIEIVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGVLLRGVQKDDIERGMVLAKPGSITPHTNFEAEVY
VLTKEEGGRHTPFFPNYRPQFYVRTTDVTGTISAFTADDGSSAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTIG
AGTVSKILK
>Mature_408_residues
ARAKFERNKDHANIGTVGHVDHGKTTLTAAITMALAAQGGGKAKSYEDIDAAPEEKARGITINTAHVEYETENRHYAHVD
CPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLAKQVGVPSLVVFLNKEDQVDDEELLELVELEVRELLSEY
DFPGDDIPITTGSALKAVEALVANPNIKRGEDKWVDKILALMDSVDEYMPLPERDVDKPFLMAVEDVFSITGRGTVATGR
IERGRVKVGETIEIVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGVLLRGVQKDDIERGMVLAKPGSITPHTNFEAEVYV
LTKEEGGRHTPFFPNYRPQFYVRTTDVTGTISAFTADDGSSAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTIGA
GTVSKILK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=407, Percent_Identity=53.5626535626536, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=446, Percent_Identity=29.1479820627803, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI5729864, Length=446, Percent_Identity=29.1479820627803, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI4503475, Length=452, Percent_Identity=28.5398230088496, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=28.47533632287, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI46094014, Length=442, Percent_Identity=26.9230769230769, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI194018522, Length=442, Percent_Identity=26.2443438914027, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI194018520, Length=442, Percent_Identity=26.2443438914027, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI194097354, Length=442, Percent_Identity=26.2443438914027, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI54607086, Length=338, Percent_Identity=26.9230769230769, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4503507, Length=276, Percent_Identity=25.3623188405797, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI94966754, Length=215, Percent_Identity=29.3023255813954, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=408, Percent_Identity=69.6078431372549, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1789737, Length=408, Percent_Identity=69.6078431372549, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI2367247, Length=357, Percent_Identity=29.9719887955182, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI48994988, Length=246, Percent_Identity=30.8943089430894, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789108, Length=277, Percent_Identity=29.9638989169675, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=412, Percent_Identity=50, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=400, Percent_Identity=42.75, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=28.3482142857143, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=28.3482142857143, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=448, Percent_Identity=27.9017857142857, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=452, Percent_Identity=28.0973451327434, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=452, Percent_Identity=28.0973451327434, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=441, Percent_Identity=25.8503401360544, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=281, Percent_Identity=29.8932384341637, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.0952380952381, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17565438, Length=439, Percent_Identity=24.1457858769932, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=242, Percent_Identity=27.6859504132231, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=407, Percent_Identity=61.9164619164619, Blast_Score=514, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6325337, Length=349, Percent_Identity=32.378223495702, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=349, Percent_Identity=32.378223495702, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=463, Percent_Identity=23.9740820734341, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=350, Percent_Identity=29.4285714285714, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=280, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=301, Percent_Identity=27.2425249169435, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=181, Percent_Identity=29.8342541436464, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=403, Percent_Identity=55.3349875930521, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=403, Percent_Identity=55.3349875930521, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=404, Percent_Identity=45.7920792079208, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=28.8288288288288, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=28.8288288288288, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=28.8288288288288, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=28.8288288288288, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=28.6995515695067, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=28.6995515695067, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45550900, Length=456, Percent_Identity=27.1929824561404, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI19922690, Length=311, Percent_Identity=29.903536977492, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137380, Length=444, Percent_Identity=26.8018018018018, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647048, Length=276, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24647046, Length=276, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=35.3658536585366, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_SYNP2 (B1XI63)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001735303.1
- ProteinModelPortal:   B1XI63
- SMR:   B1XI63
- GeneID:   6055464
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A2061
- HOGENOM:   HBG307581
- OMA:   ERKPHIN
- ProtClustDB:   PRK00049
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44553; Mature: 44422

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAKFERNKDHANIGTVGHVDHGKTTLTAAITMALAAQGGGKAKSYEDIDAAPEEKARG
CCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHE
LLAKQVGVPSLVVFLNKEDQVDDEELLELVELEVRELLSEYDFPGDDIPITTGSALKAVE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHH
ALVANPNIKRGEDKWVDKILALMDSVDEYMPLPERDVDKPFLMAVEDVFSITGRGTVATG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
RIERGRVKVGETIEIVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGVLLRGVQKDDIERG
CEECCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCC
MVLAKPGSITPHTNFEAEVYVLTKEEGGRHTPFFPNYRPQFYVRTTDVTGTISAFTADDG
EEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCC
SSAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTIGAGTVSKILK
CCCEEEECCCCEEEEEEHHCCHHHHHCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
ARAKFERNKDHANIGTVGHVDHGKTTLTAAITMALAAQGGGKAKSYEDIDAAPEEKARG
CCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHE
LLAKQVGVPSLVVFLNKEDQVDDEELLELVELEVRELLSEYDFPGDDIPITTGSALKAVE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHH
ALVANPNIKRGEDKWVDKILALMDSVDEYMPLPERDVDKPFLMAVEDVFSITGRGTVATG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
RIERGRVKVGETIEIVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGVLLRGVQKDDIERG
CEECCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCC
MVLAKPGSITPHTNFEAEVYVLTKEEGGRHTPFFPNYRPQFYVRTTDVTGTISAFTADDG
EEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCC
SSAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTIGAGTVSKILK
CCCEEEECCCCEEEEEEHHCCHHHHHCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA