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The map label for this gene is dam [H]

Identifier: 170078228

GI number: 170078228

Start: 1700682

End: 1701500

Strand: Reverse

Name: dam [H]

Synonym: SYNPCC7002_A1620

Alternate gene names: 170078228

Gene position: 1701500-1700682 (Counterclockwise)

Preceding gene: 170078229

Following gene: 170078227

Centisome position: 56.56

GC content: 42.12

Gene sequence:

>819_bases
ATGGCTATGATTAATTCAGAGCAGTTAAAACCGCCCCTAAAGTGGGCTGGGGGTAAGCGTTGGTTAGTCCCTAAATTACG
GTTAATTTGGCAAGACTACCAAGACTATCAATTAATTGAGCCGTTCTGTGGTGGCTTGGCGATCGCCTTGGGTTTGGTTC
CGCAAAAAGCAATTTTAAATGACGTTAATTTCCATTTGATTAATTTTTATCAGCAACTCAAAAAAGGTTTAAAAAGTAGT
ATTTTAATGGCAAATGATGCTGACTTATATTATCAATGCCGAGACCGTTTTAATGAATTAATTGAAAATAATCAGAGCGA
GACCCAAGAAGCCGCTATTTTATTTTATTATTTAAACCGGACTGGGTTTAATGGCTTGTGTCGATTCAATTCCCAGGGAA
AATTTAATGTCCCTTTCGGGCGATATCGTTCGATTAACTATAGGGATGATTTTTTTGCCTACAAAAAGTTATTTCAAGCT
TGGCAGTTTCAATGGGGAGATTTTGAGAAAATTCCAGTTGCTGACCATAGTTTTATTTATGCTGATCCGCCCTATGATGT
TGAGTTTCGTCAGTATGCCGCCGGGGGGTTTAGTTGGGCCGATCAAGAGCGATTAGCCACCTGGCTAGCAGCACAAACCG
CGCCAACAATTGCCTCTAATCAAGCCACGCCCAGAATTTTAGATCTGTATCAAAGTTTGGGCTTCACCGTGACGACCTTA
AGCGCCCCACGACGCATTGCCTGCAACGGCGATCGCACTCCGGCTAAAGAGATGCTCGCCTTCCGTAATTTGGCAGCAGA
AGATTTGTTGAATACATAG

Upstream 100 bases:

>100_bases
AATGATGTTTTAAACCGTTAGTCGTTAGTATCGATCGACCATTGCACAGTGAAATCAGACGATGGATGGGGAACAATGAC
CAAAAAGCCCCATCTTTTTT

Downstream 100 bases:

>100_bases
GGTTTATGTAATTTATGCAACCATTTCAGGCACCCTGTAAATTGTGATACCAGTTTTCCGGCGAGAGGAACTTACCTTGA
AGGTCTGTCAACTTTTTATG

Product: adenine-specific DNA methylase

Products: NA

Alternate protein names: M.DpnIIA; Adenine-specific methyltransferase DpnIIA; M.DpnII 1 [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAMINSEQLKPPLKWAGGKRWLVPKLRLIWQDYQDYQLIEPFCGGLAIALGLVPQKAILNDVNFHLINFYQQLKKGLKSS
ILMANDADLYYQCRDRFNELIENNQSETQEAAILFYYLNRTGFNGLCRFNSQGKFNVPFGRYRSINYRDDFFAYKKLFQA
WQFQWGDFEKIPVADHSFIYADPPYDVEFRQYAAGGFSWADQERLATWLAAQTAPTIASNQATPRILDLYQSLGFTVTTL
SAPRRIACNGDRTPAKEMLAFRNLAAEDLLNT

Sequences:

>Translated_272_residues
MAMINSEQLKPPLKWAGGKRWLVPKLRLIWQDYQDYQLIEPFCGGLAIALGLVPQKAILNDVNFHLINFYQQLKKGLKSS
ILMANDADLYYQCRDRFNELIENNQSETQEAAILFYYLNRTGFNGLCRFNSQGKFNVPFGRYRSINYRDDFFAYKKLFQA
WQFQWGDFEKIPVADHSFIYADPPYDVEFRQYAAGGFSWADQERLATWLAAQTAPTIASNQATPRILDLYQSLGFTVTTL
SAPRRIACNGDRTPAKEMLAFRNLAAEDLLNT
>Mature_271_residues
AMINSEQLKPPLKWAGGKRWLVPKLRLIWQDYQDYQLIEPFCGGLAIALGLVPQKAILNDVNFHLINFYQQLKKGLKSSI
LMANDADLYYQCRDRFNELIENNQSETQEAAILFYYLNRTGFNGLCRFNSQGKFNVPFGRYRSINYRDDFFAYKKLFQAW
QFQWGDFEKIPVADHSFIYADPPYDVEFRQYAAGGFSWADQERLATWLAAQTAPTIASNQATPRILDLYQSLGFTVTTLS
APRRIACNGDRTPAKEMLAFRNLAAEDLLNT

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the DpnII endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=269, Percent_Identity=31.5985130111524, Blast_Score=107, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31380; Mature: 31249

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMINSEQLKPPLKWAGGKRWLVPKLRLIWQDYQDYQLIEPFCGGLAIALGLVPQKAILN
CCCCCCHHCCCCHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
DVNFHLINFYQQLKKGLKSSILMANDADLYYQCRDRFNELIENNQSETQEAAILFYYLNR
HCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHEEEEEEECC
TGFNGLCRFNSQGKFNVPFGRYRSINYRDDFFAYKKLFQAWQFQWGDFEKIPVADHSFIY
CCCCCEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
ADPPYDVEFRQYAAGGFSWADQERLATWLAAQTAPTIASNQATPRILDLYQSLGFTVTTL
ECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEE
SAPRRIACNGDRTPAKEMLAFRNLAAEDLLNT
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AMINSEQLKPPLKWAGGKRWLVPKLRLIWQDYQDYQLIEPFCGGLAIALGLVPQKAILN
CCCCCHHCCCCHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
DVNFHLINFYQQLKKGLKSSILMANDADLYYQCRDRFNELIENNQSETQEAAILFYYLNR
HCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHEEEEEEECC
TGFNGLCRFNSQGKFNVPFGRYRSINYRDDFFAYKKLFQAWQFQWGDFEKIPVADHSFIY
CCCCCEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
ADPPYDVEFRQYAAGGFSWADQERLATWLAAQTAPTIASNQATPRILDLYQSLGFTVTTL
ECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEE
SAPRRIACNGDRTPAKEMLAFRNLAAEDLLNT
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2989823; 3019562; 2824782; 9862809 [H]