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The map label for this gene is miaE [H]

Identifier: 170078161

GI number: 170078161

Start: 1639309

End: 1639908

Strand: Direct

Name: miaE [H]

Synonym: SYNPCC7002_A1552

Alternate gene names: 170078161

Gene position: 1639309-1639908 (Clockwise)

Preceding gene: 170078158

Following gene: 170078162

Centisome position: 54.5

GC content: 53.33

Gene sequence:

>600_bases
ATGACTGCTGCGACTTTACCCCGGATCAAGTTTCTTCAGGAGCCAACCCGCCCGGAATGGATCGAACTCGCCCTCAATAA
TTTGGATACGATTTTGCTGGATCACTCCCACTGTGAACGGAAAGCGGCAGGGGTCGCGCTGAATATGATGTTCCGGTATC
CCTCGGCGGTGGAACTAGTGAAAGCATTAACGGCGATCGCCAAGGAAGAATTAGAGCATTTCGAGCTGGTGAACCAGTGG
CTAGAACGGAAAAATATTCCCCTCGCGCCGTTGAATGCGCCCCCCTATGGCGGCCAGTTGCGCAAATTGATCCGGCCCCA
GGAACCGGAACGGCTGTTGGATTCGTTGTTAATTTCGGCGTTAATCGAAGCCCGCTCCCACGAACGTTTAGGCCTGTTGG
GGGAACATTGCCCGGAGCCGGATTTGGCAAAATTTTATCGGGGGTTAATGGCCTCGGAAGCGCGGCATTATGGGGCTTAT
TGGGTATTGGCAACGACCTATTACGATCAAGATTTGGTGAATCAACGGCTAGAGGAATTGGCGATCGCCGAAAGTGAAAT
TCTCTCGACCCTCCACCCGGAACCCCGGATCCATAGCTAG

Upstream 100 bases:

>100_bases
GTGGCCGTTCTCGCAGGAAACTTGCTCTCGATAGCGGCTATTCTGCATCGGGCTTGGGGAAACTATATACTGTTGAGTTG
TCCGCCATCTCCCCAAATCT

Downstream 100 bases:

>100_bases
AATCAACGGCAACGGCATTTACCAAGGGAATTTTGCAGGATGGATCGGGATACAAAATTTATTTTGCTGGTTTTGGGCTT
GCCCGTGGCGGGGCTGGTGT

Product: tRNA-(MS[2]IO[6]A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTAATLPRIKFLQEPTRPEWIELALNNLDTILLDHSHCERKAAGVALNMMFRYPSAVELVKALTAIAKEELEHFELVNQW
LERKNIPLAPLNAPPYGGQLRKLIRPQEPERLLDSLLISALIEARSHERLGLLGEHCPEPDLAKFYRGLMASEARHYGAY
WVLATTYYDQDLVNQRLEELAIAESEILSTLHPEPRIHS

Sequences:

>Translated_199_residues
MTAATLPRIKFLQEPTRPEWIELALNNLDTILLDHSHCERKAAGVALNMMFRYPSAVELVKALTAIAKEELEHFELVNQW
LERKNIPLAPLNAPPYGGQLRKLIRPQEPERLLDSLLISALIEARSHERLGLLGEHCPEPDLAKFYRGLMASEARHYGAY
WVLATTYYDQDLVNQRLEELAIAESEILSTLHPEPRIHS
>Mature_198_residues
TAATLPRIKFLQEPTRPEWIELALNNLDTILLDHSHCERKAAGVALNMMFRYPSAVELVKALTAIAKEELEHFELVNQWL
ERKNIPLAPLNAPPYGGQLRKLIRPQEPERLLDSLLISALIEARSHERLGLLGEHCPEPDLAKFYRGLMASEARHYGAYW
VLATTYYDQDLVNQRLEELAIAESEILSTLHPEPRIHS

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 22736; Mature: 22605

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATLPRIKFLQEPTRPEWIELALNNLDTILLDHSHCERKAAGVALNMMFRYPSAVELV
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCHHHHHH
KALTAIAKEELEHFELVNQWLERKNIPLAPLNAPPYGGQLRKLIRPQEPERLLDSLLISA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
LIEARSHERLGLLGEHCPEPDLAKFYRGLMASEARHYGAYWVLATTYYDQDLVNQRLEEL
HHHHHCCCHHCCHHCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHH
AIAESEILSTLHPEPRIHS
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TAATLPRIKFLQEPTRPEWIELALNNLDTILLDHSHCERKAAGVALNMMFRYPSAVELV
CCCCCHHHHHHCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCHHHHHH
KALTAIAKEELEHFELVNQWLERKNIPLAPLNAPPYGGQLRKLIRPQEPERLLDSLLISA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
LIEARSHERLGLLGEHCPEPDLAKFYRGLMASEARHYGAYWVLATTYYDQDLVNQRLEEL
HHHHHCCCHHCCHHCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHH
AIAESEILSTLHPEPRIHS
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]