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The map label for this gene is menE [H]

Identifier: 170077866

GI number: 170077866

Start: 1290476

End: 1291633

Strand: Direct

Name: menE [H]

Synonym: SYNPCC7002_A1250

Alternate gene names: 170077866

Gene position: 1290476-1291633 (Clockwise)

Preceding gene: 170077864

Following gene: 170077867

Centisome position: 42.9

GC content: 45.34

Gene sequence:

>1158_bases
ATGTCGTCGCTCCAAATCATTGATCAAAAAAATCCAGCCTTCTTTGTTGAAGCGTTTCAAAAGGCGATCGCCCGTCACGA
ATCCGTGTTGCTGGTCAATTCTACTTGGCAAACTTCAGAACGGACACAAATTAAATCACTCTTAGGGCAAGATCAAAGGC
CAGAACTGCTAACAAAAAAATCCCACATTTTAATTCCCACTGGCGGCACATCAGGACAGCTAAAATTGGCAATTCATACC
CCTGAAACCTTCTCTGCTTCGGCCCAGGCTTTTCAGGATTTTTTCGCTTTAGAACGGGTAAATTGCCTTTGTATTTTGCC
CCTCTACCATGTCAGCGGCTTGATGCAATGGTGGCGATCACATTTGAGTGGGGGTGACTTTAAACTCGGCGATTATCCAG
CGTTAAAACGCGGCATTTTGCCGGATTGGGATGTCAGTAATTTTTGTCTGTCCTTAGTGCCGACCCAATTACAAGTATTG
CTCCAAGTTTGCCCCCAATGGTTAGCCCAGTTCCGCTTAATATTACTCGGTGGGGCACCACCTTGGCGATCGCTCCTTGA
GCAAGCCCGGCAATTGCAGCTCCGGATTGCTCTGACCTATGGCATGACGGAAACCGCTTCCCAGGTGGTAGCCCTAAAAC
CCGCAGACTTTTTAGACGGCAAAGATTGTGCGGGCCAAGTTTTGCCCCATGCCAATTTATCTTTATCAAAAACGGGTTTG
ATTTCCATTGCGGCCCAATCGCTATTTCTCGGTTATTACCCCAATTTTGATTATCCTAAAATCTTAGAAACCGATGATGT
TGGTGCCTTTGATCAAGCGGGCTATTTACATTTGTTTGGACGCCAAAGCCAGAAGATTATTAGTGGTGGTGAAAATATTT
ATCCAGCGGAAATTGAAGCAGCAATTCTAGGGACGAAATTCGTTAAAGATGCGGCGGTTTTGGGTTTGCTAGATCCTTAC
TGGGGAGAAGTTGTCATCGCTGTGCTGAGTTTGAAAAATCCGAGTGATTTAGACAGGGTAAAAATGGCGATCGCCAAGCA
ATTAAGCCCTTATAAACAGCCAAAACATTGGTTGCTGGTTGAACAATTGCCGCGCAATGCCCAGGGAAAGTTAAACCGAA
TCAAACTCCGCCAAATGATTGAAAATCGCTATCAGTAA

Upstream 100 bases:

>100_bases
AGACAGGGCAAAAAAAGTTTCTTTGGAGAACATTATGGAATAATCAGCAACCAAGGGGCGTTTGTCTCCGTATTCTAAAG
GATTCTGCTTATTTGCTTCG

Downstream 100 bases:

>100_bases
AGGAGGAAGTTGCATTTTTGATGTAGATTTATGAAGCAAGCTTAATTTTGCCAATACATCTGCCATGACTGCCCTTAGTC
CTACCCACATCAACCCCAGC

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSSLQIIDQKNPAFFVEAFQKAIARHESVLLVNSTWQTSERTQIKSLLGQDQRPELLTKKSHILIPTGGTSGQLKLAIHT
PETFSASAQAFQDFFALERVNCLCILPLYHVSGLMQWWRSHLSGGDFKLGDYPALKRGILPDWDVSNFCLSLVPTQLQVL
LQVCPQWLAQFRLILLGGAPPWRSLLEQARQLQLRIALTYGMTETASQVVALKPADFLDGKDCAGQVLPHANLSLSKTGL
ISIAAQSLFLGYYPNFDYPKILETDDVGAFDQAGYLHLFGRQSQKIISGGENIYPAEIEAAILGTKFVKDAAVLGLLDPY
WGEVVIAVLSLKNPSDLDRVKMAIAKQLSPYKQPKHWLLVEQLPRNAQGKLNRIKLRQMIENRYQ

Sequences:

>Translated_385_residues
MSSLQIIDQKNPAFFVEAFQKAIARHESVLLVNSTWQTSERTQIKSLLGQDQRPELLTKKSHILIPTGGTSGQLKLAIHT
PETFSASAQAFQDFFALERVNCLCILPLYHVSGLMQWWRSHLSGGDFKLGDYPALKRGILPDWDVSNFCLSLVPTQLQVL
LQVCPQWLAQFRLILLGGAPPWRSLLEQARQLQLRIALTYGMTETASQVVALKPADFLDGKDCAGQVLPHANLSLSKTGL
ISIAAQSLFLGYYPNFDYPKILETDDVGAFDQAGYLHLFGRQSQKIISGGENIYPAEIEAAILGTKFVKDAAVLGLLDPY
WGEVVIAVLSLKNPSDLDRVKMAIAKQLSPYKQPKHWLLVEQLPRNAQGKLNRIKLRQMIENRYQ
>Mature_384_residues
SSLQIIDQKNPAFFVEAFQKAIARHESVLLVNSTWQTSERTQIKSLLGQDQRPELLTKKSHILIPTGGTSGQLKLAIHTP
ETFSASAQAFQDFFALERVNCLCILPLYHVSGLMQWWRSHLSGGDFKLGDYPALKRGILPDWDVSNFCLSLVPTQLQVLL
QVCPQWLAQFRLILLGGAPPWRSLLEQARQLQLRIALTYGMTETASQVVALKPADFLDGKDCAGQVLPHANLSLSKTGLI
SIAAQSLFLGYYPNFDYPKILETDDVGAFDQAGYLHLFGRQSQKIISGGENIYPAEIEAAILGTKFVKDAAVLGLLDPYW
GEVVIAVLSLKNPSDLDRVKMAIAKQLSPYKQPKHWLLVEQLPRNAQGKLNRIKLRQMIENRYQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=226, Percent_Identity=28.7610619469027, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI115511026, Length=171, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI58082049, Length=219, Percent_Identity=30.5936073059361, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI157311624, Length=230, Percent_Identity=30.4347826086957, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI157311622, Length=230, Percent_Identity=30.4347826086957, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI122937307, Length=242, Percent_Identity=29.7520661157025, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693145, Length=363, Percent_Identity=26.7217630853994, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788595, Length=324, Percent_Identity=28.7037037037037, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788107, Length=361, Percent_Identity=26.0387811634349, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786810, Length=351, Percent_Identity=25.3561253561254, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=341, Percent_Identity=33.7243401759531, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=347, Percent_Identity=28.5302593659942, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=238, Percent_Identity=24.7899159663866, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=369, Percent_Identity=24.9322493224932, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=311, Percent_Identity=26.6881028938907, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17557194, Length=337, Percent_Identity=25.5192878338279, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538037, Length=234, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=304, Percent_Identity=27.9605263157895, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=297, Percent_Identity=26.5993265993266, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323182, Length=145, Percent_Identity=30.3448275862069, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24581924, Length=349, Percent_Identity=25.214899713467, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 43037; Mature: 42906

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLQIIDQKNPAFFVEAFQKAIARHESVLLVNSTWQTSERTQIKSLLGQDQRPELLTKK
CCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHCCC
SHILIPTGGTSGQLKLAIHTPETFSASAQAFQDFFALERVNCLCILPLYHVSGLMQWWRS
CEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
HLSGGDFKLGDYPALKRGILPDWDVSNFCLSLVPTQLQVLLQVCPQWLAQFRLILLGGAP
HCCCCCEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
PWRSLLEQARQLQLRIALTYGMTETASQVVALKPADFLDGKDCAGQVLPHANLSLSKTGL
HHHHHHHHHHHHEEEEEEEECCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
ISIAAQSLFLGYYPNFDYPKILETDDVGAFDQAGYLHLFGRQSQKIISGGENIYPAEIEA
HHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCHHHHH
AILGTKFVKDAAVLGLLDPYWGEVVIAVLSLKNPSDLDRVKMAIAKQLSPYKQPKHWLLV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHH
EQLPRNAQGKLNRIKLRQMIENRYQ
HHCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSLQIIDQKNPAFFVEAFQKAIARHESVLLVNSTWQTSERTQIKSLLGQDQRPELLTKK
CCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHCCC
SHILIPTGGTSGQLKLAIHTPETFSASAQAFQDFFALERVNCLCILPLYHVSGLMQWWRS
CEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
HLSGGDFKLGDYPALKRGILPDWDVSNFCLSLVPTQLQVLLQVCPQWLAQFRLILLGGAP
HCCCCCEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
PWRSLLEQARQLQLRIALTYGMTETASQVVALKPADFLDGKDCAGQVLPHANLSLSKTGL
HHHHHHHHHHHHEEEEEEEECCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
ISIAAQSLFLGYYPNFDYPKILETDDVGAFDQAGYLHLFGRQSQKIISGGENIYPAEIEA
HHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCHHHHH
AILGTKFVKDAAVLGLLDPYWGEVVIAVLSLKNPSDLDRVKMAIAKQLSPYKQPKHWLLV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHH
EQLPRNAQGKLNRIKLRQMIENRYQ
HHCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA