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The map label for this gene is sigB [H]

Identifier: 170077819

GI number: 170077819

Start: 1246031

End: 1247017

Strand: Reverse

Name: sigB [H]

Synonym: SYNPCC7002_A1202

Alternate gene names: 170077819

Gene position: 1247017-1246031 (Counterclockwise)

Preceding gene: 170077821

Following gene: 170077816

Centisome position: 41.46

GC content: 52.48

Gene sequence:

>987_bases
ATGCCTAAAGTAAATCTTCAACAAGCCCCCAATAACGGCAAAAGTTCTGCGGATATGGTGCGTACCTATCTCCGGGAAAT
CGGTCGGGTGCCCTTGTTAACCCACGAAGAAGAAATTGTCTATGGGAAACAGGTTCAGCGCCTGATGGCCCTCCAGGAAA
AACGCCAAACCCTAGAAGAATCCCTTGATCGCGAAGCAACCCGTGAAGAATGGGCCGAGGCCGCTCAACTGACACCGAAG
CAACTCCAACGGGATCTCCAAATCGGGGAACGGGCAAAGCGCAAGATGATTGAAGCAAATCTCCGGCTTGTGGTGGCGAT
CGCCAAGAAATATCAAAAGCGCAACCTAGAATTCTTAGATTTGATCCAAGAAGGCAGCCTTGGTTTAGAACGGGGTGTCG
AGAAGTTTGACCCCACAAAAGGGTATAAGTTCTCCACCTATGCCTACTGGTGGATTCGCCAGGCGATCACCCGGGCGATC
GCCCAACAGGGTCGCACGATTCGACTCCCGATCCACATTACCGAAAAGCTCAACAAAATTAAGAAAACCCAGCGGCAACT
GGCCCAAGACCTAGGCCGGGCGGCCACCATCGCTGAAATCGCCGAAGCCCTCGAATTAGAGCCGGCCCAAATCCGTGAAT
ATTTCCGTGTTTCCCGGCACCCAATCTCCCTCGATGTCCGGGTTGGCGATAATCAAGATACCGAGTTAATGGACTTACTC
GAAGACAGCGGCGAATCCCCCGACAACAACGTCACCTATGAGTTGCTCAAACAGGATTTGCGCGACCTGATCGCGGATTT
GACCCCCCAGCAACAGGAAGTGATTATTCTGCGCTTTGGCCTGAACGATGGCAAAGAACTATCCCTGGCAAAAATTGGCA
AACGCATGAACCTCAGTCGGGAACGGGTACGCCAGCTAGAACGCCAGGCTTTGAATCATCTGCGGCGCCGGCGATCGCAA
ATTCGTGAATATCTGGTTGCCAGCTAA

Upstream 100 bases:

>100_bases
TAGATTGTGACCTACATGATGATTGTTTTGTAAAGTATCTGCCCCCCTGGGCCGCACCATATAGTTCTTCGTTCTTTAGA
TTTAAGGAGTTAAAGCGTAA

Downstream 100 bases:

>100_bases
ATTTTTCAACTTTTCACCATGACTTAGGCATTCTGAGATGGGGCATTCTCCCCATCTTTTTTTTGTCCATCTGGACGGCT
AATAAAAAAGATTTTTAATA

Product: sigma-70 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPKVNLQQAPNNGKSSADMVRTYLREIGRVPLLTHEEEIVYGKQVQRLMALQEKRQTLEESLDREATREEWAEAAQLTPK
QLQRDLQIGERAKRKMIEANLRLVVAIAKKYQKRNLEFLDLIQEGSLGLERGVEKFDPTKGYKFSTYAYWWIRQAITRAI
AQQGRTIRLPIHITEKLNKIKKTQRQLAQDLGRAATIAEIAEALELEPAQIREYFRVSRHPISLDVRVGDNQDTELMDLL
EDSGESPDNNVTYELLKQDLRDLIADLTPQQQEVIILRFGLNDGKELSLAKIGKRMNLSRERVRQLERQALNHLRRRRSQ
IREYLVAS

Sequences:

>Translated_328_residues
MPKVNLQQAPNNGKSSADMVRTYLREIGRVPLLTHEEEIVYGKQVQRLMALQEKRQTLEESLDREATREEWAEAAQLTPK
QLQRDLQIGERAKRKMIEANLRLVVAIAKKYQKRNLEFLDLIQEGSLGLERGVEKFDPTKGYKFSTYAYWWIRQAITRAI
AQQGRTIRLPIHITEKLNKIKKTQRQLAQDLGRAATIAEIAEALELEPAQIREYFRVSRHPISLDVRVGDNQDTELMDLL
EDSGESPDNNVTYELLKQDLRDLIADLTPQQQEVIILRFGLNDGKELSLAKIGKRMNLSRERVRQLERQALNHLRRRRSQ
IREYLVAS
>Mature_327_residues
PKVNLQQAPNNGKSSADMVRTYLREIGRVPLLTHEEEIVYGKQVQRLMALQEKRQTLEESLDREATREEWAEAAQLTPKQ
LQRDLQIGERAKRKMIEANLRLVVAIAKKYQKRNLEFLDLIQEGSLGLERGVEKFDPTKGYKFSTYAYWWIRQAITRAIA
QQGRTIRLPIHITEKLNKIKKTQRQLAQDLGRAATIAEIAEALELEPAQIREYFRVSRHPISLDVRVGDNQDTELMDLLE
DSGESPDNNVTYELLKQDLRDLIADLTPQQQEVIILRFGLNDGKELSLAKIGKRMNLSRERVRQLERQALNHLRRRRSQI
REYLVAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is a component of the biological clock pathway that affects the circadian expression of a subset of ge

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=253, Percent_Identity=45.4545454545455, Blast_Score=237, Evalue=7e-64,
Organism=Escherichia coli, GI1789098, Length=299, Percent_Identity=38.7959866220736, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1789871, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788231, Length=207, Percent_Identity=28.5024154589372, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38130; Mature: 37999

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00715 SIGMA70_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVNLQQAPNNGKSSADMVRTYLREIGRVPLLTHEEEIVYGKQVQRLMALQEKRQTLEE
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLDREATREEWAEAAQLTPKQLQRDLQIGERAKRKMIEANLRLVVAIAKKYQKRNLEFLD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LIQEGSLGLERGVEKFDPTKGYKFSTYAYWWIRQAITRAIAQQGRTIRLPIHITEKLNKI
HHHHCCCCHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
KKTQRQLAQDLGRAATIAEIAEALELEPAQIREYFRVSRHPISLDVRVGDNQDTELMDLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
EDSGESPDNNVTYELLKQDLRDLIADLTPQQQEVIILRFGLNDGKELSLAKIGKRMNLSR
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCH
ERVRQLERQALNHLRRRRSQIREYLVAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKVNLQQAPNNGKSSADMVRTYLREIGRVPLLTHEEEIVYGKQVQRLMALQEKRQTLEE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLDREATREEWAEAAQLTPKQLQRDLQIGERAKRKMIEANLRLVVAIAKKYQKRNLEFLD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LIQEGSLGLERGVEKFDPTKGYKFSTYAYWWIRQAITRAIAQQGRTIRLPIHITEKLNKI
HHHHCCCCHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
KKTQRQLAQDLGRAATIAEIAEALELEPAQIREYFRVSRHPISLDVRVGDNQDTELMDLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
EDSGESPDNNVTYELLKQDLRDLIADLTPQQQEVIILRFGLNDGKELSLAKIGKRMNLSR
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCH
ERVRQLERQALNHLRRRRSQIREYLVAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8665856; 1368828 [H]