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The map label for this gene is rne [H]

Identifier: 170077410

GI number: 170077410

Start: 814563

End: 816644

Strand: Direct

Name: rne [H]

Synonym: SYNPCC7002_A0788

Alternate gene names: 170077410

Gene position: 814563-816644 (Clockwise)

Preceding gene: 170077409

Following gene: 170077411

Centisome position: 27.08

GC content: 52.98

Gene sequence:

>2082_bases
ATGCCTAAGCAAATTATTATTGCAGAGCAGCATCAGATTGCTGCCGTTTTTTGGGAAGACCAAATCCAAGAAATTGTCGT
ATCCACCGGTGCCCAACAGGTCGGTGACATCTATCTCGGTATCGTCGAAAACGTCATTCCCGGCATTGACGCGGCCTTTG
TCAACATCGGTGACACCGAAAAAAACGGTTTCATCCACGTCAGCGATCTCGGCCCCATTCGACTCAGAAAAAATTCCAGC
TCAATCACCGAACTCCTCGAACCCCAACAAAAAGCCTTGGTTCAGGTGATGAAAGAACCCACGGGAAACAAAGGGCCACG
GCTCACAGGCAATATTACCCTCCCCGGTCGCTACGTCGTCCTGATGCCCTACAGCAAAGGAGTTAACCTTTCCCGCCGCA
TCAGTAGTGATAGCGAACGTAACCGTCTGCGGGCCCTCGCCGTGCTGATGAAACCTCCCGGCATGGGACTTTTGGTGCGG
ACTGAGGCCGAAGGTACCGACGAAGAAGCGATCATCGAAGATTTAGAACTGCTCCAAAAACAGTGGGAAGCGATCCAGCA
GCAGGCAAACTACGGCCGTCCCCCGACCCTGATCAACCGCGATGACGACTTTATTCAGCGGGTGCTGCGGGACATGTACA
GCGATGAAGTGAACCGCATCGTGGTGGATTCTGCCAGCGGTATGAAACGGGTGAAAAGCCAGCTCATGAACTGGGAAGGG
GGGAAAGCGCCCGACGGTTTGTTAATTGATCATCACCGCGAAAAGCAATCGATTCTGGACTATTTCCGGGTGAATGCGGC
CATTCGTGAAGCCCTCAAGCCCAGGGTTGAGTTGCCTTCTGGGGGCTACATCATCATTGAACCCACCGAAGCTTTAACTG
TCATTGACGTAAACTCTGGTTCTTTTACTAAATCCGCCACCGCACGGGAAACGGTGCTTTGGACGAACTATGAAGCGGCC
ACAGAAATTGCGCGGCAACTGCGGTTACGCAACATTGGTGGGGTAATCGTGGTGGATTTCATCGACATGGATTCTCGCCA
TGATCAGTTGAAGTTGCTGGAGCATTTTACAAGTATTCTCAAGGCGGATAAGGCCCGACCCCAGATCGCCCAGTTGTCTG
AATTGGGTTTGGTAGAACTGACTCGCAAACGGCAGGGTCAAAATATTTATGAACTGTTCGGTAAGCCCTGCGCCCACTGT
AATGGCCTCGGTCACTTGGTACATTTACCCGGTGAAGAGGAACCAGTACCACTCCAGTTAAATATTCTGTCTTCTCTCTC
GCTCCAGGGCGGGGCGCGGACACCGGGAGAAAGTCGGCCTGTACTGTCTGGGGGACGGCGCGGCACCACCAGCCACGGAC
GACGGATTACATCACGGGTTGCTCGGAGCAAGCTGATTAATGCAGGGAATACGCCTGCGGCAGAGCCGTTAAATCTGGTG
CATCATCCCGATTATCAGGAACAAAGCAGCAATAATAATAATCGTCGTCGTCGCCGGCGATCGCCAGAGGTTTCGAGTAA
GCCGGTGCTCCGGGACGAAAAAGAAACCCAAGATAATGGTGGCGATCGCCCCTCTGCTGAGACGCCAACAAAAGAAAAAA
AAGAAACAAAAACGTCTTCGCCCCAAGACAACAGCAATACGTCTTCCCGCTATGACCGCCGCGAACGGAGCCGTTACCGC
CGTGACGAGCCCAAAGAAACCGTCCAGGTGGAAATGACGGAGCAGGAAAAAAATGTCTATGCCCTGATTGGGGTATCCCC
CCTGGTGCGTTTAGACGCTGCTGGGAAAAAAGACCCCCGCTCAACGATTATCGAAATTGTGCGACCGGGAAGCGCCAAAG
AAACCGATGCCACTTCGGAAAAGGTAACACCAGTCGAAAATTTAGAGCCGCAACAACTCCAGATTGAGACCCCTGAAACT
GGAGAGGTCGCCCCCGAAGCAGACGACACAGCTCCCCAAACTGCTCAACCCGTAGAAACAAAAGTTTCACCCCAGGCGGA
ACCGGAAGCAGCAGAAAAAGACGCTGAAGAAAAAACCAATGGCCGTCGCCGTCGCCGTCGCCGTTCTTCCGCCAGTAGTT
AA

Upstream 100 bases:

>100_bases
CAAGAAATATCCAGCCTAGTTTCAAAGCATTTATTTCAGACAACAGAACTTGCATAGAACAGTTTTTTCAGCATTAAGCG
AAAAAAATCGAGGAATTTGA

Downstream 100 bases:

>100_bases
AACCCAATTACCGTTAACCTAAGGCAGTGGCAACACTGCTTTTTTCTTTTGGGGGTGTTTTCGCCCCCTCACTCTGACCC
CAAAAATTCCCTTAAAAAGC

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MPKQIIIAEQHQIAAVFWEDQIQEIVVSTGAQQVGDIYLGIVENVIPGIDAAFVNIGDTEKNGFIHVSDLGPIRLRKNSS
SITELLEPQQKALVQVMKEPTGNKGPRLTGNITLPGRYVVLMPYSKGVNLSRRISSDSERNRLRALAVLMKPPGMGLLVR
TEAEGTDEEAIIEDLELLQKQWEAIQQQANYGRPPTLINRDDDFIQRVLRDMYSDEVNRIVVDSASGMKRVKSQLMNWEG
GKAPDGLLIDHHREKQSILDYFRVNAAIREALKPRVELPSGGYIIIEPTEALTVIDVNSGSFTKSATARETVLWTNYEAA
TEIARQLRLRNIGGVIVVDFIDMDSRHDQLKLLEHFTSILKADKARPQIAQLSELGLVELTRKRQGQNIYELFGKPCAHC
NGLGHLVHLPGEEEPVPLQLNILSSLSLQGGARTPGESRPVLSGGRRGTTSHGRRITSRVARSKLINAGNTPAAEPLNLV
HHPDYQEQSSNNNNRRRRRRSPEVSSKPVLRDEKETQDNGGDRPSAETPTKEKKETKTSSPQDNSNTSSRYDRRERSRYR
RDEPKETVQVEMTEQEKNVYALIGVSPLVRLDAAGKKDPRSTIIEIVRPGSAKETDATSEKVTPVENLEPQQLQIETPET
GEVAPEADDTAPQTAQPVETKVSPQAEPEAAEKDAEEKTNGRRRRRRRSSASS

Sequences:

>Translated_693_residues
MPKQIIIAEQHQIAAVFWEDQIQEIVVSTGAQQVGDIYLGIVENVIPGIDAAFVNIGDTEKNGFIHVSDLGPIRLRKNSS
SITELLEPQQKALVQVMKEPTGNKGPRLTGNITLPGRYVVLMPYSKGVNLSRRISSDSERNRLRALAVLMKPPGMGLLVR
TEAEGTDEEAIIEDLELLQKQWEAIQQQANYGRPPTLINRDDDFIQRVLRDMYSDEVNRIVVDSASGMKRVKSQLMNWEG
GKAPDGLLIDHHREKQSILDYFRVNAAIREALKPRVELPSGGYIIIEPTEALTVIDVNSGSFTKSATARETVLWTNYEAA
TEIARQLRLRNIGGVIVVDFIDMDSRHDQLKLLEHFTSILKADKARPQIAQLSELGLVELTRKRQGQNIYELFGKPCAHC
NGLGHLVHLPGEEEPVPLQLNILSSLSLQGGARTPGESRPVLSGGRRGTTSHGRRITSRVARSKLINAGNTPAAEPLNLV
HHPDYQEQSSNNNNRRRRRRSPEVSSKPVLRDEKETQDNGGDRPSAETPTKEKKETKTSSPQDNSNTSSRYDRRERSRYR
RDEPKETVQVEMTEQEKNVYALIGVSPLVRLDAAGKKDPRSTIIEIVRPGSAKETDATSEKVTPVENLEPQQLQIETPET
GEVAPEADDTAPQTAQPVETKVSPQAEPEAAEKDAEEKTNGRRRRRRRSSASS
>Mature_692_residues
PKQIIIAEQHQIAAVFWEDQIQEIVVSTGAQQVGDIYLGIVENVIPGIDAAFVNIGDTEKNGFIHVSDLGPIRLRKNSSS
ITELLEPQQKALVQVMKEPTGNKGPRLTGNITLPGRYVVLMPYSKGVNLSRRISSDSERNRLRALAVLMKPPGMGLLVRT
EAEGTDEEAIIEDLELLQKQWEAIQQQANYGRPPTLINRDDDFIQRVLRDMYSDEVNRIVVDSASGMKRVKSQLMNWEGG
KAPDGLLIDHHREKQSILDYFRVNAAIREALKPRVELPSGGYIIIEPTEALTVIDVNSGSFTKSATARETVLWTNYEAAT
EIARQLRLRNIGGVIVVDFIDMDSRHDQLKLLEHFTSILKADKARPQIAQLSELGLVELTRKRQGQNIYELFGKPCAHCN
GLGHLVHLPGEEEPVPLQLNILSSLSLQGGARTPGESRPVLSGGRRGTTSHGRRITSRVARSKLINAGNTPAAEPLNLVH
HPDYQEQSSNNNNRRRRRRSPEVSSKPVLRDEKETQDNGGDRPSAETPTKEKKETKTSSPQDNSNTSSRYDRRERSRYRR
DEPKETVQVEMTEQEKNVYALIGVSPLVRLDAAGKKDPRSTIIEIVRPGSAKETDATSEKVTPVENLEPQQLQIETPETG
EVAPEADDTAPQTAQPVETKVSPQAEPEAAEKDAEEKTNGRRRRRRRSSASS

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=379, Percent_Identity=36.1477572559367, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082235, Length=396, Percent_Identity=38.3838383838384, Blast_Score=234, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 77200; Mature: 77069

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQIIIAEQHQIAAVFWEDQIQEIVVSTGAQQVGDIYLGIVENVIPGIDAAFVNIGDTE
CCCCEEEECCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KNGFIHVSDLGPIRLRKNSSSITELLEPQQKALVQVMKEPTGNKGPRLTGNITLPGRYVV
CCCEEEEECCCCEEEECCCHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LMPYSKGVNLSRRISSDSERNRLRALAVLMKPPGMGLLVRTEAEGTDEEAIIEDLELLQK
EEECCCCCCHHHHHCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCHHHHHHHHHHHHH
QWEAIQQQANYGRPPTLINRDDDFIQRVLRDMYSDEVNRIVVDSASGMKRVKSQLMNWEG
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHCCCC
GKAPDGLLIDHHREKQSILDYFRVNAAIREALKPRVELPSGGYIIIEPTEALTVIDVNSG
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCEEEEEECCCC
SFTKSATARETVLWTNYEAATEIARQLRLRNIGGVIVVDFIDMDSRHDQLKLLEHFTSIL
CCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
KADKARPQIAQLSELGLVELTRKRQGQNIYELFGKPCAHCNGLGHLVHLPGEEEPVPLQL
HHCCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHCCCHHHCCCCCCEEECCCCCCCCCEEE
NILSSLSLQGGARTPGESRPVLSGGRRGTTSHGRRITSRVARSKLINAGNTPAAEPLNLV
EEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHC
HHPDYQEQSSNNNNRRRRRRSPEVSSKPVLRDEKETQDNGGDRPSAETPTKEKKETKTSS
CCCCCHHHCCCCCHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHCCCC
PQDNSNTSSRYDRRERSRYRRDEPKETVQVEMTEQEKNVYALIGVSPLVRLDAAGKKDPR
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCEEECCCCCCCHH
STIIEIVRPGSAKETDATSEKVTPVENLEPQQLQIETPETGEVAPEADDTAPQTAQPVET
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
KVSPQAEPEAAEKDAEEKTNGRRRRRRRSSASS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PKQIIIAEQHQIAAVFWEDQIQEIVVSTGAQQVGDIYLGIVENVIPGIDAAFVNIGDTE
CCCEEEECCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KNGFIHVSDLGPIRLRKNSSSITELLEPQQKALVQVMKEPTGNKGPRLTGNITLPGRYVV
CCCEEEEECCCCEEEECCCHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LMPYSKGVNLSRRISSDSERNRLRALAVLMKPPGMGLLVRTEAEGTDEEAIIEDLELLQK
EEECCCCCCHHHHHCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCHHHHHHHHHHHHH
QWEAIQQQANYGRPPTLINRDDDFIQRVLRDMYSDEVNRIVVDSASGMKRVKSQLMNWEG
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHCCCC
GKAPDGLLIDHHREKQSILDYFRVNAAIREALKPRVELPSGGYIIIEPTEALTVIDVNSG
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCEEEEEECCCC
SFTKSATARETVLWTNYEAATEIARQLRLRNIGGVIVVDFIDMDSRHDQLKLLEHFTSIL
CCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
KADKARPQIAQLSELGLVELTRKRQGQNIYELFGKPCAHCNGLGHLVHLPGEEEPVPLQL
HHCCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHCCCHHHCCCCCCEEECCCCCCCCCEEE
NILSSLSLQGGARTPGESRPVLSGGRRGTTSHGRRITSRVARSKLINAGNTPAAEPLNLV
EEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHC
HHPDYQEQSSNNNNRRRRRRSPEVSSKPVLRDEKETQDNGGDRPSAETPTKEKKETKTSS
CCCCCHHHCCCCCHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHCCCC
PQDNSNTSSRYDRRERSRYRRDEPKETVQVEMTEQEKNVYALIGVSPLVRLDAAGKKDPR
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCEEECCCCCCCHH
STIIEIVRPGSAKETDATSEKVTPVENLEPQQLQIETPETGEVAPEADDTAPQTAQPVET
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
KVSPQAEPEAAEKDAEEKTNGRRRRRRRSSASS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]