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The map label for this gene is nifS [H]

Identifier: 170077409

GI number: 170077409

Start: 813046

End: 814182

Strand: Direct

Name: nifS [H]

Synonym: SYNPCC7002_A0787

Alternate gene names: 170077409

Gene position: 813046-814182 (Clockwise)

Preceding gene: 170077408

Following gene: 170077410

Centisome position: 27.03

GC content: 52.77

Gene sequence:

>1137_bases
ATGAAGCGTCCCATTTATCTCGATAACCATGCGACAACTCCCGTTGATCCGCAGGTATTAGAAGCGATGTTGCCCTATTT
TACGGAGCAGTTTGGTAATCCTGCGAGTGCTGGCCATGTTTATGGGTGGGAAGCGGTGGCGGCAGTGGAACGATCCAGAG
AAATGATCGCCAGTAGTATTGGGGCAGAACCAGAGGAAATTGTTTTTACGAGCGGGGCGACGGAGGCAAATAATTTAGCG
ATTAAAGGAGTAGCTGAGGCTTATTTTGACAAAGGCAGGCATATTGTTACGGTGCAAACGGAACATCGAGCGGTGCTTGA
CCCCTGTGCCTATTTGGAAACTTTGGGGTTTGAGGTGACTCATCTGCCCGTGCAAGCCAATGGCTTATTGGATTTATCTT
TGTTGGAAAAAGCACTCCGTCAAGATACGGTGCTGGTCTCGGTAATGGCGGCCAATAACGAGATTGGCGTACTCCAACCC
GTCGCCGAGATTGGCACCCTCTGTCATGATCGAGGTGTTTTATTTCACTGTGACGCGGCCCAGGCGATCGCCAAAATTCC
CCTAAATGTCCAAACCCAAAATATTGACCTGCTGTCCCTGACAGCCCATAAAATTTATGGCCCCAAGGGAATCGGTGCGT
TGTACGTGCGCCGCAAAAATCCCCGGGTCAATCTTGCGCCCCAACTCCACGGCGGCGGCCAAGAACGGGGGCGGCGATCG
GGCACCCTCTATACGCCGCAAATTGTTGGCTTTGGCAAGGCCGTCGAAATTGGTCTGGCCCAGATGGATGAAGAAAATGC
CAAGATTAAACTATTACGCGATCGCCTTTGGCAGCAACTCCAACCCCTAGAGGGCATTTATCTAAACGGCGACCTCGACC
AACGGCTCCTCCATAATCTCAACATCAGTATCGAAAATATTGATGGCACAGCCCTCCTTTTGGCACTGCGCAATACCGTC
GCCCTTTCCGCTGGTTCCGCCTGTAGTTCCGGTAAACCCTCCCATGTCCTTACTGCCCTGGGGCGATCGCCAGCCCTTTG
TCGCGCCTCCCTCCGCTTTGGTTTAGGCCGTTTCACCACTGCTACCGATATCGATCACGTCGCCTACACCCTACAGCACA
CCCTCCACAGCCTCTGA

Upstream 100 bases:

>100_bases
CAGCAAGCCCAAAGTTTACCAATGCCGACTTTTGCTCAATCATTGACTTTAAGGGGAGATGAGATTTTTGCTTGGTTAAA
AAATTAGGCTATTTTTTGTG

Downstream 100 bases:

>100_bases
AAAATGCCCACCAAGACCCCATGAAAGTGATCCAAAGTGAACACTTGGCTTTAGAATGGAATCTGAGGCGATTGCAAATA
TGCCGCTAAACCTTCCTGTA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKRPIYLDNHATTPVDPQVLEAMLPYFTEQFGNPASAGHVYGWEAVAAVERSREMIASSIGAEPEEIVFTSGATEANNLA
IKGVAEAYFDKGRHIVTVQTEHRAVLDPCAYLETLGFEVTHLPVQANGLLDLSLLEKALRQDTVLVSVMAANNEIGVLQP
VAEIGTLCHDRGVLFHCDAAQAIAKIPLNVQTQNIDLLSLTAHKIYGPKGIGALYVRRKNPRVNLAPQLHGGGQERGRRS
GTLYTPQIVGFGKAVEIGLAQMDEENAKIKLLRDRLWQQLQPLEGIYLNGDLDQRLLHNLNISIENIDGTALLLALRNTV
ALSAGSACSSGKPSHVLTALGRSPALCRASLRFGLGRFTTATDIDHVAYTLQHTLHSL

Sequences:

>Translated_378_residues
MKRPIYLDNHATTPVDPQVLEAMLPYFTEQFGNPASAGHVYGWEAVAAVERSREMIASSIGAEPEEIVFTSGATEANNLA
IKGVAEAYFDKGRHIVTVQTEHRAVLDPCAYLETLGFEVTHLPVQANGLLDLSLLEKALRQDTVLVSVMAANNEIGVLQP
VAEIGTLCHDRGVLFHCDAAQAIAKIPLNVQTQNIDLLSLTAHKIYGPKGIGALYVRRKNPRVNLAPQLHGGGQERGRRS
GTLYTPQIVGFGKAVEIGLAQMDEENAKIKLLRDRLWQQLQPLEGIYLNGDLDQRLLHNLNISIENIDGTALLLALRNTV
ALSAGSACSSGKPSHVLTALGRSPALCRASLRFGLGRFTTATDIDHVAYTLQHTLHSL
>Mature_378_residues
MKRPIYLDNHATTPVDPQVLEAMLPYFTEQFGNPASAGHVYGWEAVAAVERSREMIASSIGAEPEEIVFTSGATEANNLA
IKGVAEAYFDKGRHIVTVQTEHRAVLDPCAYLETLGFEVTHLPVQANGLLDLSLLEKALRQDTVLVSVMAANNEIGVLQP
VAEIGTLCHDRGVLFHCDAAQAIAKIPLNVQTQNIDLLSLTAHKIYGPKGIGALYVRRKNPRVNLAPQLHGGGQERGRRS
GTLYTPQIVGFGKAVEIGLAQMDEENAKIKLLRDRLWQQLQPLEGIYLNGDLDQRLLHNLNISIENIDGTALLLALRNTV
ALSAGSACSSGKPSHVLTALGRSPALCRASLRFGLGRFTTATDIDHVAYTLQHTLHSL

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=375, Percent_Identity=53.0666666666667, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=35.8173076923077, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI48994898, Length=382, Percent_Identity=52.3560209424084, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1787970, Length=389, Percent_Identity=27.5064267352185, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789175, Length=230, Percent_Identity=29.5652173913043, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=368, Percent_Identity=50.8152173913043, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17533177, Length=320, Percent_Identity=35, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=367, Percent_Identity=53.133514986376, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI20129463, Length=369, Percent_Identity=55.0135501355014, Blast_Score=412, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41024; Mature: 41024

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPIYLDNHATTPVDPQVLEAMLPYFTEQFGNPASAGHVYGWEAVAAVERSREMIASSI
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GAEPEEIVFTSGATEANNLAIKGVAEAYFDKGRHIVTVQTEHRAVLDPCAYLETLGFEVT
CCCCHHEEEECCCCCCCCEEEEHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCEEE
HLPVQANGLLDLSLLEKALRQDTVLVSVMAANNEIGVLQPVAEIGTLCHDRGVLFHCDAA
EEEEECCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEECHH
QAIAKIPLNVQTQNIDLLSLTAHKIYGPKGIGALYVRRKNPRVNLAPQLHGGGQERGRRS
HHHHHCCCEEEECCEEEEEEEHHHHCCCCCCCEEEEEECCCCEEECCCCCCCCHHHCCCC
GTLYTPQIVGFGKAVEIGLAQMDEENAKIKLLRDRLWQQLQPLEGIYLNGDLDQRLLHNL
CCEECCHHCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHC
NISIENIDGTALLLALRNTVALSAGSACSSGKPSHVLTALGRSPALCRASLRFGLGRFTT
CEEEEECCCCEEEEEHHHHHEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCC
ATDIDHVAYTLQHTLHSL
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRPIYLDNHATTPVDPQVLEAMLPYFTEQFGNPASAGHVYGWEAVAAVERSREMIASSI
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GAEPEEIVFTSGATEANNLAIKGVAEAYFDKGRHIVTVQTEHRAVLDPCAYLETLGFEVT
CCCCHHEEEECCCCCCCCEEEEHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCEEE
HLPVQANGLLDLSLLEKALRQDTVLVSVMAANNEIGVLQPVAEIGTLCHDRGVLFHCDAA
EEEEECCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEECHH
QAIAKIPLNVQTQNIDLLSLTAHKIYGPKGIGALYVRRKNPRVNLAPQLHGGGQERGRRS
HHHHHCCCEEEECCEEEEEEEHHHHCCCCCCCEEEEEECCCCEEECCCCCCCCHHHCCCC
GTLYTPQIVGFGKAVEIGLAQMDEENAKIKLLRDRLWQQLQPLEGIYLNGDLDQRLLHNL
CCEECCHHCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHC
NISIENIDGTALLLALRNTVALSAGSACSSGKPSHVLTALGRSPALCRASLRFGLGRFTT
CEEEEECCCCEEEEEHHHHHEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCC
ATDIDHVAYTLQHTLHSL
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA