Click here to switch to the map view.

The map label for this gene is lipA [H]

Identifier: 170077407

GI number: 170077407

Start: 811535

End: 812440

Strand: Direct

Name: lipA [H]

Synonym: SYNPCC7002_A0785

Alternate gene names: 170077407

Gene position: 811535-812440 (Clockwise)

Preceding gene: 170077406

Following gene: 170077408

Centisome position: 26.98

GC content: 52.21

Gene sequence:

>906_bases
GTGGCGGTAAAACCAGATTGGCTCAGAGTAAAGGCCCCCCAATGGCAACGGGTTGGCAGCGTCAAAGAAATTTTACGGGA
CCTCAGCCTCAACACCGTCTGCGAAGAAGCCTCCTGTCCCAACATTGGCGAATGTTTTAATGCAGGCACCGCCACCTTTT
TGATTATGGGGCCAGCCTGCACCCGTGCTTGCCCCTACTGCGACATTGACTTCGACAAAGTGCCCCGGGGTTTAGATCCC
ACAGAACCCGACAATCTTGCCGAAGCCGTCCGCCGTCTGAATCTCAATCATGTCGTGATCACTTCCGTCAACCGTGACGA
TCTCCCCGATGGTGGCGCAAGCCAATTTGTAAAATGTATCGAACGAACTCGCGAACTGTCACCCAAAACCACCATTGAAG
TCTTGATTCCTGACCTCTGCGGTAATTGGGACGCCCTCAAAACGATCCTTGATGCTGCCCCCGAAGTGTTGAACCACAAC
ACCGAGACCGTCCCCCGTCTTTATAAAAAAACGCGTCCCCAAGGGGATTATCAACGTTCCCTGGAGTTACTCCAGCGCAC
CCGCGAGCTAACCCCCTGGGTTTACACCAAATCGGGGATTATGGTTGGCCTTGGAGAAACCGATGCCGAAGTGCGTGAAG
TAATGCGTGATCTGCGAGCCGTAGACTGTGACATTCTCACCATTGGTCAATACCTCCAGCCCACCCAAAAACATTTAGGG
GTGCAAGATTTTATTACCCCAGCACAATTTGACGCTTGGCGGGAATATGGTGAATCCCTCGGCTTTTTGCAAGTAGTATC
TAGCCCCCTCACCCGCAGTTCCTACCATGCCGAACAGGTGCGGCGCTTAATGGAAACCCACCCACGATCTAAGCCAGAAC
TCAGTCAGTTACATTCACTAAAATAA

Upstream 100 bases:

>100_bases
CATCCCGAAAAACAGAACCTATACTAACACAGGTAAACCTTGCTACGATAAAAACTTGTCCGTTAAGTCCAAAATTCTCA
AAAGATAAGGAGCGATCGCC

Downstream 100 bases:

>100_bases
AAAATAGTTTCCATCTACGGTACGTTCCATGGTGCAAGCACTAAGTCGCTCAATGACCCTAGAAGAATTCCTGGCGCTAC
CAGAGACTAAACCTGCCAAC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MAVKPDWLRVKAPQWQRVGSVKEILRDLSLNTVCEEASCPNIGECFNAGTATFLIMGPACTRACPYCDIDFDKVPRGLDP
TEPDNLAEAVRRLNLNHVVITSVNRDDLPDGGASQFVKCIERTRELSPKTTIEVLIPDLCGNWDALKTILDAAPEVLNHN
TETVPRLYKKTRPQGDYQRSLELLQRTRELTPWVYTKSGIMVGLGETDAEVREVMRDLRAVDCDILTIGQYLQPTQKHLG
VQDFITPAQFDAWREYGESLGFLQVVSSPLTRSSYHAEQVRRLMETHPRSKPELSQLHSLK

Sequences:

>Translated_301_residues
MAVKPDWLRVKAPQWQRVGSVKEILRDLSLNTVCEEASCPNIGECFNAGTATFLIMGPACTRACPYCDIDFDKVPRGLDP
TEPDNLAEAVRRLNLNHVVITSVNRDDLPDGGASQFVKCIERTRELSPKTTIEVLIPDLCGNWDALKTILDAAPEVLNHN
TETVPRLYKKTRPQGDYQRSLELLQRTRELTPWVYTKSGIMVGLGETDAEVREVMRDLRAVDCDILTIGQYLQPTQKHLG
VQDFITPAQFDAWREYGESLGFLQVVSSPLTRSSYHAEQVRRLMETHPRSKPELSQLHSLK
>Mature_300_residues
AVKPDWLRVKAPQWQRVGSVKEILRDLSLNTVCEEASCPNIGECFNAGTATFLIMGPACTRACPYCDIDFDKVPRGLDPT
EPDNLAEAVRRLNLNHVVITSVNRDDLPDGGASQFVKCIERTRELSPKTTIEVLIPDLCGNWDALKTILDAAPEVLNHNT
ETVPRLYKKTRPQGDYQRSLELLQRTRELTPWVYTKSGIMVGLGETDAEVREVMRDLRAVDCDILTIGQYLQPTQKHLGV
QDFITPAQFDAWREYGESLGFLQVVSSPLTRSSYHAEQVRRLMETHPRSKPELSQLHSLK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=43.6170212765958, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=43.4426229508197, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI1786846, Length=285, Percent_Identity=49.1228070175439, Blast_Score=288, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=252, Percent_Identity=44.047619047619, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=39.7163120567376, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI221513272, Length=279, Percent_Identity=42.6523297491039, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33973; Mature: 33842

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKPDWLRVKAPQWQRVGSVKEILRDLSLNTVCEEASCPNIGECFNAGTATFLIMGPAC
CCCCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCCCEEEEEECCHH
TRACPYCDIDFDKVPRGLDPTEPDNLAEAVRRLNLNHVVITSVNRDDLPDGGASQFVKCI
HHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
ERTRELSPKTTIEVLIPDLCGNWDALKTILDAAPEVLNHNTETVPRLYKKTRPQGDYQRS
HHHHHCCCCCHHHEEEHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
LELLQRTRELTPWVYTKSGIMVGLGETDAEVREVMRDLRAVDCDILTIGQYLQPTQKHLG
HHHHHHHHHCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHCC
VQDFITPAQFDAWREYGESLGFLQVVSSPLTRSSYHAEQVRRLMETHPRSKPELSQLHSL
HHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
K
C
>Mature Secondary Structure 
AVKPDWLRVKAPQWQRVGSVKEILRDLSLNTVCEEASCPNIGECFNAGTATFLIMGPAC
CCCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCCCEEEEEECCHH
TRACPYCDIDFDKVPRGLDPTEPDNLAEAVRRLNLNHVVITSVNRDDLPDGGASQFVKCI
HHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
ERTRELSPKTTIEVLIPDLCGNWDALKTILDAAPEVLNHNTETVPRLYKKTRPQGDYQRS
HHHHHCCCCCHHHEEEHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
LELLQRTRELTPWVYTKSGIMVGLGETDAEVREVMRDLRAVDCDILTIGQYLQPTQKHLG
HHHHHHHHHCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHCC
VQDFITPAQFDAWREYGESLGFLQVVSSPLTRSSYHAEQVRRLMETHPRSKPELSQLHSL
HHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]